Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CoA | enhances luminescent activity of the wild-type enzyme about 2fold, highest activity at 1.2 mM CoA. For the N-terminal domain the effect of CoA is modest, 1.1fold enhancement, highest activity at 0.01 mM CoA. Addition of CoA at higher concentrations inhibits the luminescence activity | Photinus pyralis |
Cloned (Comment) | Organism |
---|---|
Nn-terminal domain, expression in Escherichia coli | Photinus pyralis |
Protein Variants | Comment | Organism |
---|---|---|
DELTA438-550 | the purified N-terminal domain 1-437 has luminescence activity by itself, and binds to substrates ATP and luciferin with reduced affinity | Photinus pyralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | above 0.1 mM, N-terminal domain | Photinus pyralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0072 | - |
Luciferin | wild-type enzyme | Photinus pyralis | |
0.026 | - |
Luciferin | N-terminal domain | Photinus pyralis | |
0.23 | - |
ATP | wild-type enzyme | Photinus pyralis | |
6.9 | - |
ATP | N-terminal domain | Photinus pyralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal concentration for wild-type enzyme and N-terminal domain: 10 mM | Photinus pyralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, N-terminal domain, SDS-PAGE | Photinus pyralis |
61200 | - |
x * 61200, wild-type enzyme, SDS-PAGE | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
luciferin + O2 + ATP | - |
Photinus pyralis | oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, N-terminal domain, SDS-PAGE | Photinus pyralis |
? | x * 61200, wild-type enzyme, SDS-PAGE | Photinus pyralis |