Protein Variants | Comment | Organism |
---|---|---|
F149 | residue participates in stabilization of the coelenterazine peroxide and the triggering of photon emission by linking the third EF-hand to Trp-129 and His-169 coelenterazine binding residues | Aequorea victoria |
H27N | 18fold increase of wild-type activity | Aequorea victoria |
H27N/Q168R | 13fold increase of wild-type activity | Aequorea victoria |
K17R | 13fold increase of wild-type activity | Aequorea victoria |
K17R/H27N/Q168R | 11fold increase of wild-type activity | Aequorea victoria |
K17R/L170I | 8fold increase of wild-type activity | Aequorea victoria |
K17R/N26D | 14fold increase of wild-type activity | Aequorea victoria |
K17R/N26D/Q168R | 14fold increase of wild-type activity | Aequorea victoria |
K17R/Q168R | 25fold increase of wild-type activity | Aequorea victoria |
L170I | 16fold increase of wild-type activity, an increase of the photoprotein lifetime at 37°C and increased thermostability | Aequorea victoria |
additional information | mutations with increased luminescence intensity neighbor the His-16 or His-169 coelenterazine binding residues or are located in the first EF-hand | Aequorea victoria |
N26D | 13fold increase of wild-type activity | Aequorea victoria |
N26D/Q168R | 8fold increase of wild-type activity | Aequorea victoria |
Q168 | residue participates in stabilization of the coelenterazine peroxide and the triggering of photon emission by linking the third EF-hand to Trp-129 and His-169 coelenterazine binding residues | Aequorea victoria |
Q168R | 11fold increase of wild-type activity, increase of the photoprotein lifetime at 37°C and increased thermostablility | Aequorea victoria |
Q168R/L170I | mutant displays increased thermostablility | Aequorea victoria |
V25A | 10fold increase of wild-type activity | Aequorea victoria |
V25I/L170I | 24fold increase of wild-type activity | Aequorea victoria |
V25I/Q168R | 20fold increase of wild-type activity | Aequorea victoria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aequorea victoria | Q6J4T7 | - |
- |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | - |
wild-type, 50% inactivation of aequorin bioluminescence by a 30-min heat shock | Aequorea victoria |
48 | - |
mutant L170I, 50% inactivation of aequorin bioluminescence by a 30-min heat shock | Aequorea victoria |
53 | - |
mutant Q168R, 50% inactivation of aequorin bioluminescence by a 30-min heat shock | Aequorea victoria |
57 | - |
mutant Q168R/L170I, 50% inactivation of aequorin bioluminescence by a 30-min heat shock | Aequorea victoria |