Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethylxanthate | 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity | Cupriavidus necator |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.05 mM, 80% of initial activity, 0.1 mM, 61% of initial activity | Cupriavidus necator | |
ethylxanthate | 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity | Cupriavidus necator | |
additional information | addition of cysteine results in a minor decrease of activity | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.7 | - |
3-Mercaptopropanoate | pH 6.5, 30°C | Cupriavidus necator |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron content of 0.38 mol Fe(II) per mol of enzyme | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46800 | - |
gel filtration | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | Q0K029 | - |
- |
Cupriavidus necator DSM 428 | Q0K029 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.2 | - |
pH 6.5, 30°C | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopropanoate + O2 | - |
Cupriavidus necator | 3-sulfinopropanoate | - |
? | |
L-cysteine + O2 | reaction of EC 1.13.11.20 | Cupriavidus necator | 3-sulfinoalanine | - |
? | |
additional information | 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine | Cupriavidus necator | ? | - |
- |
|
additional information | 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine | Cupriavidus necator DSM 428 | ? | - |
- |
|
additional information | 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 22580, calculated from sequence | Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
CdoA | - |
Cupriavidus necator |
H16_B1863 | - |
Cupriavidus necator |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.82 | - |
3-Mercaptopropanoate | pH 6.5, 30°C | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Cupriavidus necator |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cupriavidus necator | calculated from sequence | - |
5.8 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.144 | - |
3-Mercaptopropanoate | pH 6.5, 30°C | Cupriavidus necator |