Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, 1.1fold activation | Xanthomonas sp. 35Y | |
additional information | enzymatic cleavage of rubber by the purified proteindoes not require addition of any soluble cofactors or metal ions | Xanthomonas sp. 35Y |
Application | Comment | Organism |
---|---|---|
analysis | development of an in vitro enzyme assay for oxidative rubber degradation based on high-performance liquid chromatography analysis and spectroscopic detection of product carbonyl functions after derivatization with dinitrophenylhydrazone | Xanthomonas sp. 35Y |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-S-octyl-beta-D-thioglucopyranoside | 0.5%, complete inhibition | Xanthomonas sp. 35Y | |
2,2'-dipyridyl | 10 mM, 85% inhibition | Xanthomonas sp. 35Y | |
2-mercaptoethanol | 5 mM, 2% residual activity; 5 mM, complete inhibition | Xanthomonas sp. 35Y | |
3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate | 0.5%, complete inhibition | Xanthomonas sp. 35Y | |
alpha,alpha-bipyridyl | 10 mM, 15% residual activity | Xanthomonas sp. 35Y | |
carbon monoxide | - |
Xanthomonas sp. 35Y | |
cyanide | - |
Xanthomonas sp. 35Y | |
dithiothreitol | 5 mM, 6% residual activity; 5 mM, 94% inhibition | Xanthomonas sp. 35Y | |
N-ethylmaleimide | 10 mM, 31% residual activity; 10 mM, 69% inhibition | Xanthomonas sp. 35Y | |
octyl beta-glucoside | 0.5% mM, 2% residual activity | Xanthomonas sp. 35Y | |
octyl-beta-glucoside | 0.5%, complete inhibition | Xanthomonas sp. 35Y | |
p-chloromercuribenzoate | 10 mM, 24% inhibition | Xanthomonas sp. 35Y | |
Plysurf A210G | 0.5%, complete inhibition | Xanthomonas sp. 35Y | |
SDS | 0.5%, 2% residual activity; 0.5%, complete inhibition | Xanthomonas sp. 35Y | |
sodium cholate | 0.5%, 68% inhibition | Xanthomonas sp. 35Y | |
Tiron | 10 mM, 27% inhibition | Xanthomonas sp. 35Y | |
Triton X-100 | 0.5%, complete inhibition | Xanthomonas sp. 35Y | |
Tween 80 | 0.5%, complete inhibition; 0.5% mM, 2% residual activity | Xanthomonas sp. 35Y |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Xanthomonas sp. 35Y | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | catalytic reaction does not require addition of any soluble cofactors or metal ions | Xanthomonas sp. 35Y |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
gel filtration | Xanthomonas sp. 35Y |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas sp. 35Y | Q7X0P3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Xanthomonas sp. 35Y |
from culture fluid | Xanthomonas sp. 35Y |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture fluid | - |
Xanthomonas sp. 35Y | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-1,4-polyisoprene + n O2 | - |
Xanthomonas sp. 35Y | n 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al | - |
? | |
additional information | enzyme degrades both natural rubber latex and chemosynthetic poly(cis-1,4-isoprene) in vitro by oxidative cleavage of the double bonds of poly(cis-1,4-isoprene). Enzymatic cleavage of rubber by the purified protein is strictly dependent on the presence of oxygen | Xanthomonas sp. 35Y | ? | - |
? | |
poly(cis-1,4-isoprene) + O2 | degradation by oxidative cleavage of the double bonds of poly(cis-1,4-isoprene). 12-Oxo-4,8-dimethyltrideca-4,8-diene-1-al is identified as the major cleavage product. There is a homologous series of minor metabolites that differ from the major degradation product only in the number of repetitive isoprene units between terminal functions, CHO-CH2O and OCH2-COCH3 | Xanthomonas sp. 35Y | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
rubber latex + O2 | degradation by oxidative cleavage of the double bonds of poly(cis-1,4-isoprene). 12-Oxo-4,8-dimethyltrideca-4,8-diene-1-al is identified as the major cleavage product. There is a homologous series of minor metabolites that differ from the major degradation product only in the number of repetitive isoprene units between terminal functions, CHO-CH2O and OCH2-COCH3. Enzymatic cleavage of rubber by the purified protein is strictly dependent on the presence of oxygen | Xanthomonas sp. 35Y | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, SDS-PAGE | Xanthomonas sp. 35Y |
Synonyms | Comment | Organism |
---|---|---|
roxA | - |
Xanthomonas sp. 35Y |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Xanthomonas sp. 35Y |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: about 55% of maximal activity, 50°C: about 60% of maximal activity | Xanthomonas sp. 35Y |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Xanthomonas sp. 35Y |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | pH 6.5: about 60% of maximal activity, pH 7.5: about 60% of maximal activity | Xanthomonas sp. 35Y |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | 1.9 heme per mol of protein, presence of two heme-binding motifs (CXXCH) for covalent attachment of heme to the protein | Xanthomonas sp. 35Y | |
heme | the enzyme contains approximately 2 mol of heme per mol of RoxA | Xanthomonas sp. 35Y | |
additional information | catalytic reaction does not require addition of any soluble cofactors or metal ions | Xanthomonas sp. 35Y | |
additional information | enzymatic cleavage of rubber by the purified proteindoes not require addition of any soluble cofactors or metal ions | Xanthomonas sp. 35Y |