Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | required | Homo sapiens | |
methylene blue | required | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene IDO1, recombinant expression His-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
gene IDO2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a IDO2 construct encoding an N-terminal truncated version of the predicted protein sequence consisting of residues 14-420 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-methyl-D-tryptophan | competitive inhibition of IDO2, antitumour activity, selectivity of 1-methyltryptophan inhibition of IDO1 and IDO2 | Homo sapiens | |
1-methyl-L-tryptophan | competitive inhibition of IDO1, antitumour activity, selectivity of 1-methyl L-tryptophan inhibition of IDO1 and IDO2; competitive inhibition of IDO2, antitumour activity, selectivity of 1-methyltryptophan inhibition of IDO1 and IDO2 | Homo sapiens | |
D-tryptophan | low inhibition | Homo sapiens | |
L-tryptophan | - |
Homo sapiens | |
additional information | no inhibition of IDO1 by 1-methy-D-tryptophan; no inhibition of IDO1 by L-tryptophan | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
0.006 | - |
5-fluoro-DL-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.017 | - |
5-hydroxy-L-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.0209 | - |
L-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.07 | - |
1-methyl-L-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.098 | - |
5-methyl-DL-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.296 | - |
D-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.547 | - |
5-methoxy-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.66 | - |
1-methyl-D-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.696 | - |
1-methyl-L-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.747 | - |
1-methyl-D-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
1.57 | - |
5-methyl-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
1.768 | - |
5-fluoro-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
3.457 | - |
6-methyl-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
3.609 | - |
D-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
6.809 | - |
L-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + O2 | Homo sapiens | - |
N-formyl-L-kynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14902 | - |
- |
Homo sapiens | Q6ZQW0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged IDO1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Homo sapiens |
recombinant His-tagged IDO2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methyl-D-tryptophan + O2 | - |
Homo sapiens | N-methyl-N-formyl-D-kynurenine | - |
? | |
1-methyl-L-tryptophan + O2 | - |
Homo sapiens | N-methyl-N-formyl-L-kynurenine | - |
? | |
5-fluoro-DL-tryptophan + O2 | - |
Homo sapiens | 5-fluoro-N-formyl-DL-kynurenine | - |
? | |
5-hydroxy-L-tryptophan + O2 | - |
Homo sapiens | 5-hydroxy-N-formyl-L-kynurenine | - |
? | |
5-methoxy-DL-tryptophan + O2 | - |
Homo sapiens | 5-methoxy-N-formyl-DL-kynurenine | - |
? | |
5-methyl-DL-tryptophan + O2 | - |
Homo sapiens | 5-methyl-N-formyl-DL-kynurenine | - |
? | |
5-methyl-DL-tryptophan + O2 | high activity | Homo sapiens | 5-methyl-N-formyl-DL-kynurenine | - |
? | |
6-methyl-DL-tryptophan + O2 | - |
Homo sapiens | 6-methyl-N-formyl-DL-kynurenine | - |
? | |
D-tryptophan + O2 | - |
Homo sapiens | N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | - |
Homo sapiens | N-formyl-L-kynurenine | - |
? | |
additional information | substrate specificity, overview. Replacement of the 5-methoxy group by a methyl group has a negative influence in the binding of the molecule, decreasing its affinity threefold. 5-HO-L-Trp and melatonin are no substrates for IDO2 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDO1 | - |
Homo sapiens |
IDO2 | - |
Homo sapiens |
indoleamine 2,3-dioxygenase-1 | - |
Homo sapiens |
indoleamine 2,3-dioxygenase-2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
1-methyl-D-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.0103 | - |
D-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.011 | - |
1-methyl-L-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.025 | - |
5-hydroxy-L-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.049 | - |
5-fluoro-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.062 | - |
1-methyl-L-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.095 | - |
1-methyl-D-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.103 | - |
L-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.108 | - |
6-methyl-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.161 | - |
5-methoxy-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.36 | - |
5-methyl-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.76 | - |
5-fluoro-DL-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
2.7 | - |
D-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
2.97 | - |
L-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
3.78 | - |
5-methyl-DL-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Homo sapiens |
7.5 | - |
- |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
1-methyl-L-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.05 | - |
L-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
0.3 | - |
1-methyl-L-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.543 | - |
D-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO1 | Homo sapiens | |
3.3 | - |
1-methyl-D-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
12 | - |
D-tryptophan | pH 6.5, 25°C, recombinant enzyme IDO2 | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | indoleamine 2,3-dioxygenase-2 (IDO2) is one of three enzymes, alongside tryptophan 2,3-dioxygenase (EC 1.13.11.11) and indoleamine 2,3-dioxygenase (IDO1), that catalyse dioxygenation of L-tryptophan as the first step in the kynurenine pathway | Homo sapiens |
additional information | human indoleamine 2,3-dioxygenase-2 substrate specificity and inhibition characteristics are distinct from those of indoleamine 2,3-dioxygenase-1 | Homo sapiens |
physiological function | the enzyme IDO2 might have an immunomodulatory role unrelated to that of IDO1 | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
D-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.007 | - |
1-methyl-D-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.015 | - |
L-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.016 | - |
1-methyl-L-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.028 | - |
5-fluoro-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.031 | - |
6-methyl-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.229 | - |
5-methyl-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens | |
0.294 | - |
5-methoxy-DL-tryptophan | pH 7.5, 25°C, recombinant enzyme IDO2 | Homo sapiens |