Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mesorhizobium loti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
4-pyridoxate | pH 8.0, temperature not specified in the publication | Mesorhizobium loti |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mesorhizobium loti | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
sucrose density centrifugation | Mesorhizobium loti |
57000 | - |
gel filtration | Mesorhizobium loti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-pyridoxate + 2,6-dichloroindophenol | Mesorhizobium loti | - |
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol | - |
? | |
4-pyridoxate + 2,6-dichloroindophenol | Mesorhizobium loti MAFF303099 | - |
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium loti | Q988C9 | - |
- |
Mesorhizobium loti MAFF303099 | Q988C9 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.9 | - |
pH 8.0, temperature not specified in the publication | Mesorhizobium loti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-pyridoxate + 2,6-dichloroindophenol | - |
Mesorhizobium loti | 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol | - |
? | |
4-pyridoxate + 2,6-dichloroindophenol | - |
Mesorhizobium loti MAFF303099 | 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol | - |
? | |
additional information | very poor activity with substrates pyridoxal, pyridoxine and 4-pyridoxic acid lactone, and simple alpha-hydroxy acids, such as lactic acid and glycolic acid | Mesorhizobium loti | ? | - |
? | |
additional information | very poor activity with substrates pyridoxal, pyridoxine and 4-pyridoxic acid lactone, and simple alpha-hydroxy acids, such as lactic acid and glycolic acid | Mesorhizobium loti MAFF303099 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 59000, SDS-PAGE, 1 * 58921, calculated | Mesorhizobium loti |
Synonyms | Comment | Organism |
---|---|---|
mlr6792 | - |
Mesorhizobium loti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Mesorhizobium loti |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
54% of maximum activity | Mesorhizobium loti |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable for 10 min | Mesorhizobium loti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
4-pyridoxate | pH 8.0, temperature not specified in the publication | Mesorhizobium loti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Mesorhizobium loti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol of FAD per mol of subunit. Purified protein is yellowish and shows absorption maxima near 380nm and 455 nm | Mesorhizobium loti |