Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and Pichia pastoris | Rasamsonia emersonii |
expressed in Escherichia coli and Pichia pastoris | Thermoascus crustaceus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, SDS-PAGE | Thermoascus crustaceus |
60000 | - |
x * 60000, SDS-PAGE | Rasamsonia emersonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rasamsonia emersonii | A0A1E1GL61 | - |
- |
Rasamsonia emersonii NBRC 31232 | A0A1E1GL61 | - |
- |
Thermoascus crustaceus | A0A1E1GL87 | - |
- |
Thermoascus crustaceus NBRC 9129 | A0A1E1GL87 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Rasamsonia emersonii |
glycoprotein | - |
Thermoascus crustaceus |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Rasamsonia emersonii |
Ni-NTA agarose column chromatography | Thermoascus crustaceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | 11.5% activity compared to D-glucose | Rasamsonia emersonii | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | 60.1% activity compared to D-glucose | Thermoascus crustaceus | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | 60.1% activity compared to D-glucose | Thermoascus crustaceus NBRC 9129 | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | 11.5% activity compared to D-glucose | Rasamsonia emersonii NBRC 31232 | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | high substrate specificity for D-glucose | Rasamsonia emersonii | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | high substrate specificity for D-glucose | Thermoascus crustaceus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | high substrate specificity for D-glucose | Thermoascus crustaceus NBRC 9129 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | high substrate specificity for D-glucose | Rasamsonia emersonii NBRC 31232 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichloroindophenol | 24.8% activity compared to D-glucose | Rasamsonia emersonii | D-xylono-1,5-lactone + 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichloroindophenol | 24.8% activity compared to D-glucose | Rasamsonia emersonii NBRC 31232 | D-xylono-1,5-lactone + 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichlorophenolindophenol | 27.6% activity compared to D-glucose | Thermoascus crustaceus | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichlorophenolindophenol | 27.6% activity compared to D-glucose | Thermoascus crustaceus NBRC 9129 | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltotriose + 2,6-dichlorophenolindophenol | 3.4% activity compared to D-glucose | Rasamsonia emersonii | maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltotriose + 2,6-dichlorophenolindophenol | 6.8% activity compared to D-glucose | Thermoascus crustaceus | maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltotriose + 2,6-dichlorophenolindophenol | 6.8% activity compared to D-glucose | Thermoascus crustaceus NBRC 9129 | maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltotriose + 2,6-dichlorophenolindophenol | 3.4% activity compared to D-glucose | Rasamsonia emersonii NBRC 31232 | maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose | Rasamsonia emersonii | ? | - |
? | |
additional information | no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose | Thermoascus crustaceus | ? | - |
? | |
additional information | no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose | Thermoascus crustaceus NBRC 9129 | ? | - |
? | |
additional information | no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose | Rasamsonia emersonii NBRC 31232 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Rasamsonia emersonii |
? | x * 58000, SDS-PAGE | Thermoascus crustaceus |
Synonyms | Comment | Organism |
---|---|---|
FAD-dependent glucose dehydrogenase | - |
Rasamsonia emersonii |
FAD-dependent glucose dehydrogenase | - |
Thermoascus crustaceus |
FAD-GDH | - |
Rasamsonia emersonii |
FAD-GDH | - |
Thermoascus crustaceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | - |
Thermoascus crustaceus |
65 | 70 | - |
Rasamsonia emersonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | after heat treatment at 40-70°C for 15 min, unglycosylated enzyme maintains nearly 100% activity at temperatures up to 45°C, but completely loses activity at 60°C, while glycosylated enzyme displays high thermostability, maintaining 89% activity at 60°C. The melting temperature of the glycosylated enzyme is 66.4°C | Rasamsonia emersonii |
40 | 62 | unglycosylated enzym e is stable at temperatures up to 45°C , while glycosylatedenzyme maintains 75% activity at 60°C. The melting temperature of the glycosylated enzyme is 62.5°C | Thermoascus crustaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Rasamsonia emersonii |
5 | - |
- |
Thermoascus crustaceus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 9 | the enzyme remains stable between pH 2.0 and 9.0 | Rasamsonia emersonii |
2 | 9 | the enzyme remains stable between pH 2.0 and 9.0 | Thermoascus crustaceus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rasamsonia emersonii | |
FAD | - |
Thermoascus crustaceus |