Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | 3.8fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer | Burkholderia cepacia | |
propan-2-ol | 2fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer | Burkholderia cepacia |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Burkholderia cepacia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by 1% Triton X-100, 1% Tween 20, 1% sodium cholate and 1% CHAPS | Brevibacterium sterolicum | |
additional information | not inhibited by 1% Triton X-100, 1% Tween 20, 1% sodium cholate and 1% CHAPS | Burkholderia cepacia | |
additional information | not inhibited by 1% Triton X-100, 1% Tween 20, 1% sodium cholate and 1% CHAPS | Nocardia erythropolis | |
additional information | not inhibited by 1% Triton X-100, 1% Tween 20, 1% sodium cholate and 1% CHAPS | Pseudomonas sp. | |
Sarcosyl | 1%, 88% inhibition | Brevibacterium sterolicum | |
Sarcosyl | 1%, 56% inhibition | Streptomyces sp. | |
SDS | 0.1% complete inhibition | Brevibacterium sterolicum | |
SDS | 0.1%, 20% inhibition, 1%, 41% inhibition | Burkholderia cepacia | |
SDS | 0.1% complete inhibition | Nocardia erythropolis | |
SDS | 0.1% complete inhibition | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Pseudomonas sp. | - |
cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Streptomyces sp. | - |
cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Burkholderia cepacia | - |
cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Nocardia erythropolis | - |
cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Brevibacterium sterolicum | - |
cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Burkholderia cepacia ST-200 | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sterolicum | - |
- |
- |
Burkholderia cepacia | - |
- |
- |
Burkholderia cepacia ST-200 | - |
- |
- |
Nocardia erythropolis | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Streptomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, butyl-toyopearl | Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.9 | - |
- |
Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Pseudomonas sp. | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Streptomyces sp. | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Burkholderia cepacia | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Nocardia erythropolis | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Burkholderia cepacia ST-200 | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Pseudomonas sp. | cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Streptomyces sp. | cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Burkholderia cepacia | cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Nocardia erythropolis | cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Burkholderia cepacia ST-200 | cholest-5-en-3-one + H2O2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
20% activity lost after incubation for 30 min | Burkholderia cepacia |