Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 1 mM, 100% inhibition, 0.1 mM, 62% inhibition | Brevibacterium sterolicum | |
HgCl2 | 1 mM, 68% inhibition, 0.1 mM, 38% inhibition | Brevibacterium sterolicum | |
p-chloromercuribenzoate | 1 mM, 20% inhibition, 0.1 mM, 14% inhibition | Brevibacterium sterolicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | contains no metal ions | Brevibacterium sterolicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | - |
gel filtration, sedimentation equilibrium | Brevibacterium sterolicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Brevibacterium sterolicum | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sterolicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brevibacterium sterolicum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Brevibacterium sterolicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Brevibacterium sterolicum | - |
Storage Stability | Organism |
---|---|
0-5°C, crystallized enzyme in lyophilized state, in the dark, stable for at least 10 months | Brevibacterium sterolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-cholestan-3beta-ol + O2 | 13.2% of activity with cholesterol | Brevibacterium sterolicum | 5alpha-cholestan-3-one + H2O2 | - |
? | |
beta-sitosterol + O2 | 19.7% of activity with cholesterol | Brevibacterium sterolicum | stigmast-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-5-en-3-one + H2O2 | - |
? | |
dehydroepiandrosterone + O2 | 41.4% of the activity with cholesterol | Brevibacterium sterolicum | androst-5-en-3,17-dione + H2O2 | - |
? | |
pregnenolone + O2 | 22.4% of activity with chelesterol | Brevibacterium sterolicum | 5-pregnen-3,20-dione + H2O2 | - |
? | |
stigmasterol + O2 | 10% of activity with cholesterol | Brevibacterium sterolicum | stigmasta-5,22-dien-3-one + H2O2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.0, 30 min, stable | Brevibacterium sterolicum |
60 | - |
pH 7.0, 30 min, 19% loss of activity | Brevibacterium sterolicum |
70 | - |
pH 7, 30 min, about 94% loss of activity | Brevibacterium sterolicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Brevibacterium sterolicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | about 50% of maximal activity at pH 5 and 9.5 | Brevibacterium sterolicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | 37°C, 30 min, stable | Brevibacterium sterolicum |
5 | 10 | 37°C, 30 min, stable | Brevibacterium sterolicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Brevibacterium sterolicum | |
FAD | 1 mol of FAD per mol of protein | Brevibacterium sterolicum |