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Literature summary for 1.1.3.13 extracted from

  • Portela, R.M.C.; Vogl, T.; Ebner, K.; Oliveira, R.; Glieder, A.
    Pichia pastoris alcohol oxidase 1 (AOX1) core promoter engineering by high resolution systematic mutagenesis (2018), Biotechnol. J., 13, e1700340 .
    View publication on PubMed

Application

Application Comment Organism
biotechnology in the core promoter and 5' untranslated region of the gene, mutations in the TATA box motif, regions downstream of the transcription start site or next to the start codon in the 5' UTR have a significant effect on expression. Mutations in most other regions are tolerated. These neutral core promoter positions, not affecting expression, can be exploited to introduce extrinsic sequence elements such as cloning sites and bacterial promoters Komagataella phaffii

Organism

Organism UniProt Comment Textmining
Komagataella phaffii F2QY27
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Komagataella phaffii ATCC 76273 F2QY27
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Synonyms

Synonyms Comment Organism
AOX1
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Komagataella phaffii