Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M MES (pH 5.2), 50 mM MgCl2, and 10% (w/v) monomethylether polyethylene glycol 2000 | Trametes ochracea |
Protein Variants | Comment | Organism |
---|---|---|
H167A | the mutant exhibits reduced D-glucose conversion and no D-galactose conversion activity | Trametes ochracea |
H450G | the mutant exhibits reduced D-glucose conversion and improved D-galactose conversion activity | Trametes ochracea |
H450G/V546C | the mutant exhibits reduced D-glucose conversion and improved D-galactose conversion activity | Trametes ochracea |
T169G | the mutant exhibits reduced D-glucose conversion and D-galactose conversion activities | Trametes ochracea |
T196G/V546C | the mutant exhibits reduced D-glucose conversion and D-galactose conversion activities | Trametes ochracea |
V546C | the mutant exhibits reduced D-glucose and D-galactose conversion activities | Trametes ochracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
D-galactose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
0.44 | - |
D-glucose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
0.69 | - |
D-glucose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
0.939 | - |
D-glucose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea | |
0.987 | - |
D-glucose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
2.43 | - |
D-glucose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
2.45 | - |
D-galactose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
2.48 | - |
D-galactose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
3.06 | - |
D-glucose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea | |
5.97 | - |
D-glucose | mutant enzyme H167A, pH and temperature not specified in the publication | Trametes ochracea | |
8.79 | - |
D-galactose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea | |
12 | - |
D-galactose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
46.2 | - |
D-galactose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | Trametes ochracea | - |
2-dehydro-D-glucose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | Q7ZA32 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography | Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + O2 | - |
Trametes ochracea | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-glucose + O2 | - |
Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P2O | - |
Trametes ochracea |
pyranose 2-Oxidase | - |
Trametes ochracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
D-glucose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
0.27 | - |
D-galactose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
0.38 | - |
D-galactose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
0.43 | - |
D-glucose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
2.06 | - |
D-glucose | mutant enzyme H167A, pH and temperature not specified in the publication | Trametes ochracea | |
2.51 | - |
D-galactose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea | |
3.51 | - |
D-galactose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
5.92 | - |
D-galactose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
6.57 | - |
D-galactose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea | |
12.5 | - |
D-glucose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
16.8 | - |
D-glucose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
48.1 | - |
D-glucose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea | |
88.6 | - |
D-glucose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
D-galactose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
0.142 | - |
D-galactose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea | |
0.286 | - |
D-galactose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea | |
0.35 | - |
D-glucose | mutant enzyme H167A, pH and temperature not specified in the publication | Trametes ochracea | |
0.38 | - |
D-glucose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
0.493 | - |
D-galactose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
0.94 | - |
D-galactose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
0.99 | - |
D-glucose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
1.43 | - |
D-galactose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
12.7 | - |
D-glucose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
13.5 | - |
D-glucose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
29 | - |
D-glucose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea | |
51.2 | - |
D-glucose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea |