General Stability | Organism |
---|---|
very stable in cold | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Saccharomyces cerevisiae | |
o-phenanthroline | very strong | Saccharomyces cerevisiae | |
oxalate | - |
Saccharomyces cerevisiae | |
p-Mercuriphenylsulfonate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
cytochrome c | from horse heart | Saccharomyces cerevisiae | |
0.285 | - |
D-lactate | - |
Saccharomyces cerevisiae | |
1.4 | - |
D-2-hydroxybutyrate | - |
Saccharomyces cerevisiae | |
4.45 | - |
oxidized phenazine methosulfate | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | very tightly bound to enzyme | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | - |
pyruvate + ferrocytochrome c | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1670 | - |
purified enzyme | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, as ammonium sulfate precipitate | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + ferrocytochrome c | - |
ir | |
(R)-lactate + ferricytochrome c | specific for D-isomer | Saccharomyces cerevisiae | pyruvate + ferrocytochrome c | - |
ir | |
D-2-hydroxybutyrate + cytochrome c | - |
Saccharomyces cerevisiae | 2-oxobutanoate + ferrocytochrome c | - |
? | |
D-lactate + oxidized phenazine methosulfate | - |
Saccharomyces cerevisiae | pyruvate + reduced phenazine methosulfate + H+ | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1500 | - |
D-lactate | per mol flavin | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
phosphate buffer | Saccharomyces cerevisiae |
7.5 | - |
imidazole and Tris buffer | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
stable for short term exposure (30 s) | Saccharomyces cerevisiae |
6.5 | - |
very stable | Saccharomyces cerevisiae |
7.5 | - |
complete inactivation above pH 7.5 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | cytochome c from horse-, beef- and tuna-heart more active than yeast cytochome c | Saccharomyces cerevisiae | |
FAD | - |
Saccharomyces cerevisiae | |
phenazine methosulfate | can substitute for cytochrome c | Saccharomyces cerevisiae |