Literature summary for 1.1.1.B65 extracted from

Wang, J.; Feng, J.; Li, W.; Yang, C.; Chen, X.; Bao, B.; Yang, J.; Wang, P.; Li, D.; Shi, R.
Characterization of a novel (R)-mandelate dehydrogenase from Pseudomonas putida NUST506 (2015), J. Mol. Catal. B, 120, 23-27.

No PubMed abstract available

Search for more literature for 1.1.1.B65

Activating Compound

Activating Compound	Comment	Organism	Structure
DTT	enhancement of enzyme activity of 26% and 33% in the concentration of 10 mM and 50 mM	Pseudomonas putida
Triton X-100	0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity	Pseudomonas putida

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	inhibits 4% aT 1 mM and 33% at 10 mM	Pseudomonas putida
EDTA	inhibits the enzyme activity about 68% and 89% in the concentration of 10 mM and 50 mM,respectively	Pseudomonas putida
Hg2+	strong inhibition at 1 mM	Pseudomonas putida
Li+	inhibits 17% at 10 mM	Pseudomonas putida
Mg2+	inhibits 19% at 1 mM and 31% at 10 mM	Pseudomonas putida
Mn2+	21% activation at 1 mM, and 61% inhibition at 10 mM	Pseudomonas putida
Ni2+	inhibits 39% at 1 mM and 63% at 10 mM	Pseudomonas putida
SDS	strongly inhibits the enzyme activity at 0.25-0.75%	Pseudomonas putida
Triton X-100	0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity	Pseudomonas putida
Zn2+	strong inhibition at 10 mM	Pseudomonas putida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.015	-	NADP+	pH 8.5, 30°C	Pseudomonas putida
0.018	-	NAD+	pH 8.5, 30°C	Pseudomonas putida
0.02	-	(R)-mandelate	pH 8.5, 30°C	Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	activates 44% at 1 mM and 69% at 10 mM	Pseudomonas putida
Mn2+	21% activation at 1 mM, and 61% inhibition at 10 mM	Pseudomonas putida
Na+	activates 12% at 10 mM	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(R)-mandelate + NAD+	Pseudomonas putida	-	phenylglyoxylate + NADH + H+	-	r
(R)-mandelate + NAD+	Pseudomonas putida NUST506	-	phenylglyoxylate + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China)	-
Pseudomonas putida NUST506	-	isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China)	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 11fold by ammonium sulfate fractionation and hydrophobic interaction chromatography	Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.03	-	crude enzyme extract, pH 8.5, 30°C	Pseudomonas putida
0.33	-	purified native enzyme, pH 8.5, 30°C	Pseudomonas putida

Storage Stability

Storage Stability	Organism
4°C, purified enzyme, 96 h, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-mandelate + NAD+	-	Pseudomonas putida	phenylglyoxylate + NADH + H+	-	r
(R)-mandelate + NAD+	-	Pseudomonas putida NUST506	phenylglyoxylate + NADH + H+	-	r
(R)-mandelate + NADP+	-	Pseudomonas putida	phenylglyoxylate + NADPH + H+	-	r
(R)-mandelate + NADP+	-	Pseudomonas putida NUST506	phenylglyoxylate + NADPH + H+	-	r

Subunits

Subunits	Comment	Organism
?	x * 61000, SDS-PAGE	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
RMDH	-	Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	55	activity range, profile overview	Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	-	purified enzyme, pH 8.5, 31% activity remaining after 0.5 h, and 20% after 1 h	Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.3	-	NADP+	pH 8.5, 30°C	Pseudomonas putida
0.9	-	(R)-mandelate	pH 8.5, 30°C	Pseudomonas putida
0.9	-	NAD+	pH 8.5, 30°C	Pseudomonas putida

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Pseudomonas putida

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	10	activity range, profile overview	Pseudomonas putida

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6	9	purified enzyme, the stability drops significantly at pH 8.5 and pH 9.0, the enzyme loses 85% and 94% of the activity when incubated for 3.5 h at 30°C, and it loses 11% of activity at pH 6.0 after 3.5 h at 30°C. After 96 h at 4°C, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Pseudomonas putida
NADH	-	Pseudomonas putida
NADP+	-	Pseudomonas putida
NADPH	-	Pseudomonas putida

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2026.1 (March 2026)