Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | required | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-malate | competitive versus L-tartrate and meso-tartate | Pseudomonas putida | |
L-Tartrate | competitive versus meso-tartrate and D-malate | Pseudomonas putida | |
meso-tartrate | competitive versus L-tartrate and D-malate | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
isopropylmalate | - |
Pseudomonas putida | |
0.05 | - |
D-malate | - |
Pseudomonas putida | |
0.06 | - |
meso-tartrate | - |
Pseudomonas putida | |
1 | - |
L-Tartrate | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | monovalent cation required, maximal activity with K+ and Rb+ | Pseudomonas putida | |
Mg2+ | supports catalytic activity | Pseudomonas putida | |
Mn2+ | required | Pseudomonas putida | |
Mn2+ | supports catalytic activity | Pseudomonas putida | |
Rb+ | monovalent cation required, maximal activity with K+ and Rb+ | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-(+)-tartrate + NAD+ | Pseudomonas putida | enzyme production is induced by growth on L-(+)-tartrate as the sole carbon source | oxaloglycolate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
tartrate + NAD+ = oxaloglycolate + NADH + H+ | A-side dehydrogenase, i.e. the hydride is transferred from the substrate to the pro-R position of C4 of NADH | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-malate + NAD+ | - |
Pseudomonas putida | pyruvate + CO2 + NADH | D-malate is oxidized to oxaloacetate, which remains bound to the enzyme and undergoes decarboxylation to yield pyruvate | ? | |
isopropylmalate + NAD+ | - |
Pseudomonas putida | ? | - |
? | |
L-(+)-tartrate + NAD+ | enzyme production is induced by growth on L-(+)-tartrate as the sole carbon source | Pseudomonas putida | oxaloglycolate + NADH | - |
? | |
L-tartrate + NAD+ | oxidation occurs at 2R position | Pseudomonas putida | oxaloglycolate + NADH + H+ | 3R-oxaloglycolate | ? | |
meso-tartrate + NAD+ | oxidation occurs at 2R oposition | Pseudomonas putida | oxaloglycolate + NADH + H+ | meso-tartrate is oxidized to 3S-oxaloglycolate, followed by decarboxylation to hydroxypyruvate | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
isopropylmalate | - |
Pseudomonas putida | |
0.483 | - |
L-Tartrate | - |
Pseudomonas putida | |
0.617 | - |
meso-tartrate | - |
Pseudomonas putida | |
19.7 | - |
D-malate | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor | Pseudomonas putida |