Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.1 | - |
(S)-2-octanol | isoform SDR B3, at pH 10.0 and 40°C | Cupriavidus necator | |
7.7 | - |
(S)-2-octanol | isoform SDR A5, at pH 10.0 and 40°C | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-octanol + NADPH + H+ | - |
Cupriavidus necator | ? | - |
? | |
(R)-2-octanol + NADPH + H+ | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? | |
(S)-2-octanol + NADPH + H+ | - |
Cupriavidus necator | ? | - |
? | |
(S)-2-octanol + NADPH + H+ | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? | |
2-methyl-propanol + NADPH + H+ | - |
Cupriavidus necator | ? | - |
? | |
2-methyl-propanol + NADPH + H+ | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? | |
3-methyl-butanol + NADPH + H+ | - |
Cupriavidus necator | ? | - |
? | |
benzil + NADPH + H+ | - |
Cupriavidus necator | benzoin + NADP+ | - |
? | |
butanol + NADPH + H+ | - |
Cupriavidus necator | ? | - |
? | |
additional information | no activity is detected for isoform SDR A5 with isobutyraldehyde, isovaleraldehyde, 2-heptanone, 2-phenylacetaldehyde, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 1-phenylpropane-1,2-dione, benzophenone, (R)-(-)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, rac-3-methyl-2-butanol, (S)-4-methyl-2-pentanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, (R)-2-octanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, rac-decan-1,2-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin. No activity is detected for isoform SDR B3 with isobutyraldehyde, 2-hexanone, 2-heptanone, 2-octanone, ethyl acetoacetate, acetophenone, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 4-(4-hydroxyphenyl)-2-butanone, 1-phenylpropane-1,2-dione, benzophenone, rac-benzoin, (R)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin | Cupriavidus necator | ? | - |
? | |
additional information | the enzyme accepts both NAD+ and NADP+. However, purified enzyme shows strong preference for NADP+ | Cupriavidus necator | ? | - |
? | |
additional information | no activity is detected for isoform SDR A5 with isobutyraldehyde, isovaleraldehyde, 2-heptanone, 2-phenylacetaldehyde, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 1-phenylpropane-1,2-dione, benzophenone, (R)-(-)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, rac-3-methyl-2-butanol, (S)-4-methyl-2-pentanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, (R)-2-octanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, rac-decan-1,2-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin. No activity is detected for isoform SDR B3 with isobutyraldehyde, 2-hexanone, 2-heptanone, 2-octanone, ethyl acetoacetate, acetophenone, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 4-(4-hydroxyphenyl)-2-butanone, 1-phenylpropane-1,2-dione, benzophenone, rac-benzoin, (R)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? | |
additional information | the enzyme accepts both NAD+ and NADP+. However, purified enzyme shows strong preference for NADP+ | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? | |
rac-4-methyl-2-pentanol + NADPH + H+ | - |
Cupriavidus necator | ? | - |
? | |
rac-butan-2,3-diol + NADPH + H+ | - |
Cupriavidus necator | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SDR A5 | - |
Cupriavidus necator |
SDR B3 | - |
Cupriavidus necator |
short-chain dehydrogenase/reductase | - |
Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
isoform SDR A5 | Cupriavidus necator |
50 | - |
isoform SDR B3 | Cupriavidus necator |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
(S)-2-octanol | isoform SDR B3, at pH 10.0 and 40°C | Cupriavidus necator | |
13.7 | - |
(S)-2-octanol | isoform SDR A5, at pH 10.0 and 40°C | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
for the reduction reaction | Cupriavidus necator |
11 | - |
for the oxidation reaction | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Cupriavidus necator | |
NADPH | preferred cofactor | Cupriavidus necator |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
(S)-2-octanol | isoform SDR B3, at pH 10.0 and 40°C | Cupriavidus necator | |
1.8 | - |
(S)-2-octanol | isoform SDR A5, at pH 10.0 and 40°C | Cupriavidus necator |