KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
NADPH | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH | Saccharolobus solfataricus | |
0.15 | - |
NADH | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH | Saccharolobus solfataricus | |
2.69 | - |
(S)-2-acetolactate | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH | Saccharolobus solfataricus | |
4.43 | - |
(S)-2-acetolactate | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADPH | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
440000 | - |
- |
Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-acetolactate + NADH + H+ | - |
Saccharolobus solfataricus | (R)-2,3-dihydroxyisovalerate + NAD+ | - |
? | |
(S)-2-acetolactate + NADPH + H+ | - |
Saccharolobus solfataricus | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | the dodecameric architecture is assembled by six dimeric units which are positioned on the tetrahedron edges to form a tetrahedron with T symmetry | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
KARI | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | activity increases from 40 to 80°C | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 85 | stable, based on CD analysis | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH | Saccharolobus solfataricus | |
0.01 | - |
NADH | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH | Saccharolobus solfataricus | |
0.011 | - |
(S)-2-acetolactate | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH | Saccharolobus solfataricus | |
0.013 | - |
(S)-2-acetolactate | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADPH | Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | stable, based on CD analysis | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the enzyme is bispecific to NADH and NADPH | Saccharolobus solfataricus | |
NADPH | the enzyme is bispecific to NADH and NADPH | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
(S)-2-acetolactate | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADPH | Saccharolobus solfataricus | |
0.00383 | - |
(S)-2-acetolactate | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH | Saccharolobus solfataricus | |
0.0631 | - |
NADPH | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH | Saccharolobus solfataricus | |
0.0714 | - |
NADH | pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH | Saccharolobus solfataricus |