Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-aminocarbonylcyclopropanecarboxylate | - |
Oryza sativa | |
1-cyanocyclopropanecarboxylate | - |
Oryza sativa | |
1-hydroxycyclopropanecarboxylate | - |
Oryza sativa | |
cyclopropane-1,1-dicarboxylate | a competitive slow-, tight-binding inhibitor | Oryza sativa | |
dimethylmalonate | slow-binding inhibitor | Oryza sativa | |
additional information | no inhibition by 1-aminocyclopropanecarboxylate, 1-methylcyclopropanecarboxylate, 2-methylcyclopropanecarboxylate, cyclopropane-1,2-dicarboxylate, and cyclopropanecarboxylate dimethyl ester | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0072 | - |
NADPH | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
0.042 | - |
2-acetolactate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
0.893 | - |
Hydroxypyruvate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, KM: 0.00324 mM | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q65XK0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography | Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.4 | - |
purified recombinant enzyme, substrate 2-acetolactate | Oryza sativa |
1.97 | - |
purified recombinant enzyme, substrate hydroxypyruvate | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | - |
Oryza sativa | 2,3-dihydroxyisovalerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH | - |
Oryza sativa | glycerate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KARI | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Oryza sativa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition mechanism and kinetics | Oryza sativa | |
0.0000903 | - |
cyclopropane-1,1-dicarboxylate | pH 8.0, 30°C, recombinant enzyme, versus hydroxypyruvate | Oryza sativa | |
0.000716 | - |
dimethylmalonate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
0.0056 | - |
1-hydroxycyclopropanecarboxylate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
0.0203 | - |
1-aminocarbonylcyclopropanecarboxylate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa | |
0.0585 | - |
1-cyanocyclopropanecarboxylate | pH 8.0, 30°C, recombinant enzyme | Oryza sativa |