Protein Variants | Comment | Organism |
---|---|---|
P704L/H734R | the ethanol tolerant phenotype of Clostridium thermocellum is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE). The mutant displays a complete loss of NADH-dependent activity with concomitant acquisition of NADPH-dependent activity. The reduction in specific activity with respect to NADH is far greater (about 25fold less activity) than the increase with respect to NADPH. Although total ADH activity dropps by 25fold, ethanol production does not drop significantly between strains under these conditions | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A0A0H3W5U9 | bifunctional enzyme, C-terminal section belongs to the iron-containing alcohol dehydrogenase family, N-terminal section belongs to the aldehyde dehydrogenase family | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.025 | - |
wild-type, cofoactor NADPH, pH 7.6, 55°C | Acetivibrio thermocellus |
0.052 | - |
mutant P704L/H734R, cofoactor NADH, pH 7.6, 55°C | Acetivibrio thermocellus |
0.12 | - |
mutant P704L/H734R, cofoactor NADPH, pH 7.6, 55°C | Acetivibrio thermocellus |
2.7 | - |
wild-type, cofoactor NADH, pH 7.6, 55°C | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADH + H+ | - |
Acetivibrio thermocellus | ethanol + NAD+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Acetivibrio thermocellus | ethanol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AdhE | - |
Acetivibrio thermocellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Acetivibrio thermocellus | |
NADPH | the ethanol tolerant phenotype of Clostridium thermocellum is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE). The mutant displays a complete loss of NADH-dependent activity with concomitant acquisition of NADPH-dependent activity | Acetivibrio thermocellus |