Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)RP cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method, using 3-5% (w/v) PEG 20000, 0.1 M MES pH 5.7 and 0.2 M CaCl2 | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83181 | - |
3 * 83181, calculated from amino acid sequence | Mycobacterium tuberculosis |
250000 | - |
Superose 6 gel filtration | Mycobacterium tuberculosis |
260000 | - |
dynamic light scattering | Mycobacterium tuberculosis |
290000 | - |
Superdex 200 gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Hi-Trap chelating column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-isocitrate + NAD+ | - |
Mycobacterium tuberculosis | 2-oxoglutarate + CO2 + NADH | - |
? | |
D-isocitrate + NAD+ | - |
Mycobacterium tuberculosis H37Rv | 2-oxoglutarate + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 83181, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
Icd-2 | - |
Mycobacterium tuberculosis |
isocitrate dehydrogenase 2 | - |
Mycobacterium tuberculosis |
Rv0066c | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mycobacterium tuberculosis |