Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of several ME3 knockdown cell lines, knockdown of inhibits insulin release. In the Me3 double-knockdown cells, the level of Me1 mRNA is not significantly decreased in the Me3-628(P)/Me2-725(H) and Me3-1672(P)/Me2-725(H) cell lines. However, a 32%, 49%, and 47% decrease in ME1 activity is observed in the cell lines Me3-628(P), Me3-628(P)/Me2-725(H), and Me3-628(P)/Me2-2124(H), respectively. Me3 mRNA is decreased in the Me3 single-knockdown cell line Me3-628(P), and it is lowered further in the Me3 and Me1 double-knockdown Me3628(P)/Me1-753(H) but not Me3-1672(P)/Me1-753(H). The level of Me3 mRNA in Me3-628(P)/Me1-753(H) and Me3-1672(P)/Me1-753(H) is 10% and 59% of the CHS control, respectively | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Rattus norvegicus | - |
pyruvate + CO2 + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | F1M5N4 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
INS-1 832/13 cell | - |
Rattus norvegicus | - |
insulinoma cell | - |
Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
pancreatic beta cell | ME3 enzyme activity is low in beta-cells | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Rattus norvegicus | pyruvate + CO2 + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ME3 | - |
Rattus norvegicus |
mitochondrial NADP malic enzyme | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of ME3, but not ME1 or ME2 (both EC 1.1.1.39) alone or together, inhibits insulin release stimulated by glucose, pyruvate or 2-aminobicyclo [2,2,1]heptane-2-carboxylic acid-plus-glutamine | Rattus norvegicus |
physiological function | mitochondrial NADP malic enzyme is necessary for insulin release | Rattus norvegicus |