Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fumarate | fumarate activates the NADP-ME2 forward reaction by about 15fold at 0.05 mM NADP+, but only at about 1.5fold at 0.35 mM NADP+. The trans configuration of fumarate is crucial for the activating effect | Arabidopsis thaliana | |
succinate | activates at 10 mM | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression of isozyme NADP-ME2 in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
R115A | site-directed mutagenesis of isozyme NADP-ME2, the mutant displays a marked inhibition in the presence of all the organic acids tested, also fumarate | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | inhibition of isozyme NADP-ME2 | Arabidopsis thaliana | |
Maleate | the cis isomer of fumarate, inhibition of isozyme NADP-ME2 | Arabidopsis thaliana | |
malonate | inhibition of isozyme NADP-ME2 | Arabidopsis thaliana | |
additional information | no inhibition of isozyme NADP-ME2 by tartrate | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of NADP+ and (S)-malate at different NADP+ and fumarate concentrations, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Arabidopsis thaliana | - |
pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
four malic isozymes, NADP-ME1, NADP-ME2, NADP-ME3, and NADP-ME4 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Arabidopsis thaliana | pyruvate + CO2 + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADP-dependent malic enzyme | - |
Arabidopsis thaliana |
NADP-ME | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reductive carboxylation of pyruvate | Arabidopsis thaliana |
7.5 | - |
oxidative decarboxylation of malate | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Arabidopsis thaliana | |
NADPH | dependent on | Arabidopsis thaliana |