Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Nicotiana benthamiana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Nicotiana benthamiana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
4 * 62000, tissue specific isozymes, SDS-PAGE, 4 * 70000, tissue specific isozymes, SDS-PAGE | Nicotiana benthamiana |
70000 | - |
monomeric flower isozyme, native PAGE | Nicotiana benthamiana |
140000 | - |
dimeric flower isozyme, native PAGE | Nicotiana benthamiana |
248000 | - |
tetrameric isozymes from leaves, stem and roots, native PAGE, overview | Nicotiana benthamiana |
280000 | - |
tetrameric isozymes from flower, leaves, and stem, native PAGE, overview | Nicotiana benthamiana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Nicotiana benthamiana | - |
pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana benthamiana | - |
wild-type plants and transgenic plants, expressing potyviral helper component protease HC-pro or Potato virus Y strain NTN | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermal cell | - |
Nicotiana benthamiana | - |
flower | isozyme F1 and F2 | Nicotiana benthamiana | - |
leaf | isozymes L2, L3 and L4 in older leaves, isozyme L1/R in younger leaves and roots, cell saround the midrib, as well as stomatal, epidermal, and mesophyll cells | Nicotiana benthamiana | - |
mesophyll | - |
Nicotiana benthamiana | - |
additional information | tissue-specific isozyme patterns, overview | Nicotiana benthamiana | - |
phloem | - |
Nicotiana benthamiana | - |
root | isozyme L1/R in younger leaves and roots | Nicotiana benthamiana | - |
stem | isozymes S1 and S2 | Nicotiana benthamiana | - |
stoma | - |
Nicotiana benthamiana | - |
xylem | parenchymal cell vessel | Nicotiana benthamiana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme activity is similar in Nicotiana benthamiana wild-type plants and transgenic plants, expressing potyviral helper component protease HC-pro or Potato virus Y strain NTN, overview | Nicotiana benthamiana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Nicotiana benthamiana | pyruvate + CO2 + NADPH | - |
r | |
additional information | the isozyme pattern is similar in Nicotiana benthamiana wild-type plants and transgenic plants, expressing potyviral helper component protease HC-pro or Potato virus Y strain NTN, overview | Nicotiana benthamiana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 70000, SDS-PAGE | Nicotiana benthamiana |
monomer | 1 * 70000, SDS-PAGE | Nicotiana benthamiana |
tetramer | 4 * 62000, tissue specific isozymes, SDS-PAGE, 4 * 70000, tissue specific isozymes, SDS-PAGE | Nicotiana benthamiana |
Synonyms | Comment | Organism |
---|---|---|
NADP-malic enzyme | - |
Nicotiana benthamiana |
NADP-ME | - |
Nicotiana benthamiana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Nicotiana benthamiana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Nicotiana benthamiana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Nicotiana benthamiana | |
NADPH | - |
Nicotiana benthamiana |