Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
(R)-acetoin | at pH 6.0 and 50°C | Bacillus sp. (in: Bacteria) | |
0.13 | - |
diacetyl | at pH 6.0 and 50°C | Bacillus sp. (in: Bacteria) | |
6.01 | - |
(R,R)-butane-2,3-diol | at pH 9.0 and 50°C | Bacillus sp. (in: Bacteria) | |
6.38 | - |
meso-butane-2,3-diol | at pH 9.0 and 50°C | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains zinc | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,R)-butane-2,3-diol + NAD+ | Bacillus sp. (in: Bacteria) | preferred substrate, 100% activity | (R)-acetoin + NADH + H+ | - |
? | |
(R,R)-butane-2,3-diol + NAD+ | Bacillus sp. (in: Bacteria) BRC1 | preferred substrate, 100% activity | (R)-acetoin + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | A0A075BZ18 | - |
- |
Bacillus sp. (in: Bacteria) BRC1 | A0A075BZ18 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,R)-butane-2,3-diol + NAD+ | preferred substrate, 100% activity | Bacillus sp. (in: Bacteria) | (R)-acetoin + NADH + H+ | - |
? | |
(R,R)-butane-2,3-diol + NAD+ | preferred substrate, 100% activity | Bacillus sp. (in: Bacteria) BRC1 | (R)-acetoin + NADH + H+ | - |
? | |
butane-1,2-diol + NAD+ | 76.6% activity compared to (R,R)-butane-2,3-diol | Bacillus sp. (in: Bacteria) | ? | - |
? | |
butane-1,2-diol + NAD+ | 76.6% activity compared to (R,R)-butane-2,3-diol | Bacillus sp. (in: Bacteria) BRC1 | ? | - |
? | |
butane-1,4-diol + NAD+ | 2.9% activity compared to (R,R)-butane-2,3-diol | Bacillus sp. (in: Bacteria) | ? | - |
? | |
diacetyl + NADH + H+ | 88.2% activity compared to acetoin | Bacillus sp. (in: Bacteria) | acetoin + NAD+ | - |
? | |
diacetyl + NADH + H+ | 88.2% activity compared to acetoin | Bacillus sp. (in: Bacteria) BRC1 | acetoin + NAD+ | - |
? | |
isobutanol + NAD+ | 0.4% activity compared to (R,R)-butane-2,3-diol | Bacillus sp. (in: Bacteria) | ? | - |
? | |
meso-butane-2,3-diol + NAD+ | 93.6% activity compared to (R,R)-butane-2,3-diol | Bacillus sp. (in: Bacteria) | acetoin + NADH + H+ | - |
? | |
meso-butane-2,3-diol + NAD+ | 93.6% activity compared to (R,R)-butane-2,3-diol | Bacillus sp. (in: Bacteria) BRC1 | acetoin + NADH + H+ | - |
? | |
additional information | no activity with (S,S)-butane-2,3-diol | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | no activity with (S,S)-butane-2,3-diol | Bacillus sp. (in: Bacteria) BRC1 | ? | - |
? | |
propane-1,2-diol + NAD+ | 58.6% activity compared to (R,R)-butane-2,3-diol | Bacillus sp. (in: Bacteria) | ? | - |
? | |
propane-1,3-diol + NAD+ | 1.4% activity compared to (R,R)-butane-2,3-diol | Bacillus sp. (in: Bacteria) | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BDH | - |
Bacillus sp. (in: Bacteria) |
D-2,3-BD dehydrogenase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reductive reaction | Bacillus sp. (in: Bacteria) |
9 | - |
oxidative reaction | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus sp. (in: Bacteria) |