KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
NADH | - |
Rattus norvegicus | |
0.0163 | - |
(S)-3-hydroxyoctanoyl-CoA | mitochondrial enzyme, oxidation of (S)-3-hydroxyoctanoyl-CoA | Rattus norvegicus | |
0.0169 | - |
acetoacetyl-CoA | - |
Rattus norvegicus | |
0.0286 | - |
(S)-3-hydroxyhexanoyl-CoA | mitochondrial enzyme, oxidation of (S)-3-hydroxyhexanoyl-CoA | Rattus norvegicus | |
0.0585 | - |
NAD+ | - |
Rattus norvegicus | |
0.0699 | - |
(S)-3-hydroxybutyryl-CoA | mitochondrial enzyme, oxidation of (S)-3-hydroxybutyryl-CoA | Rattus norvegicus | |
0.088 | - |
(S)-3-hydroxydecanoyl-CoA | mitochondrial enzyme, oxidation of (S)-3-hydroxydecanoyl-CoA | Rattus norvegicus | |
0.435 | - |
NADPH | - |
Rattus norvegicus | |
0.56 | - |
acetoacetyl-pantetheine | - |
Rattus norvegicus | |
11 | - |
NADP+ | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
peroxisome | - |
Rattus norvegicus | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, mitochondrial enzyme, SDS-PAGE | Rattus norvegicus |
65000 | - |
mitochondrial enzyme, gel filtration | Rattus norvegicus |
70000 | - |
peroxisomal enzyme, gel filtration | Rattus norvegicus |
77000 | - |
1 * 77000, peroxisomal enzyme, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA + NAD+ | Rattus norvegicus | - |
acetoacetyl-CoA + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
calcium phosphate gel, ammonium sulfate, CM-Sephadex, Blue Dextran-Sepharose 4B, calcium phosphate gel-cellulose, Blue Dextran-Sepharose 4B, mitochondrial enzyme | Rattus norvegicus |
phosphocellulose, ammonium sulfate, CM-cellulose, ammonium sulfate, peroxisomal enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.6 | - |
peroxisomal enzyme | Rattus norvegicus |
565 | - |
mitochondrial enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 120 mM potassium phosphate, pH 7.0, several months without loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA + NAD+ | - |
Rattus norvegicus | acetoacetyl-CoA + NADH | - |
r | |
(S)-3-hydroxybutyryl-CoA + NADP+ | low activity for mitochondrial enzyme | Rattus norvegicus | acetoacetyl-CoA + NADPH | - |
r | |
(S)-3-hydroxydecanoyl-CoA + NAD+ | - |
Rattus norvegicus | 3-ketodecanoyl-CoA + NADH | - |
r | |
(S)-3-hydroxyhexanoyl-CoA + NAD+ | - |
Rattus norvegicus | 3-oxohexanoyl-CoA + NADH + H+ | - |
r | |
3-ketooctanoyl-CoA + NADH | - |
Rattus norvegicus | (S)-3-hydroxyoctanoyl-CoA + NAD+ | - |
? | |
acetoacetyl-pantetheine + NADH | - |
Rattus norvegicus | (S)-3-hydroxybutyryl-pantetheine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, mitochondrial enzyme, SDS-PAGE | Rattus norvegicus |
monomer | 1 * 77000, peroxisomal enzyme, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
mitochondrial enzyme, reduction of acetoacetyl-CoA | Rattus norvegicus |
6.2 | - |
peroxisomal enzyme, reduction of acetoacetyl-CoA | Rattus norvegicus |
9.6 | - |
mitochondrial enzyme, oxidation of (S)-3-hydroxybutyryl-CoA | Rattus norvegicus |
9.8 | - |
peroxisomal enzyme, oxidation of (S)-3-hydroxybutyryl-CoA | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus | |
NADP+ | - |
Rattus norvegicus |