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Literature summary for 1.1.1.300 extracted from

  • Belyaeva, O.V.; Lee, S.A.; Kolupaev, O.V.; Kedishvili, N.Y.
    Identification and characterization of retinoid-active short-chain dehydrogenases/reductases in Drosophila melanogaster (2009), Biochim. Biophys. Acta, 1790, 1266-1273.
    View publication on PubMed
Search for more literature for 1.1.1.300

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 cells Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information CG2070-expessing microsomes do not show saturation with up to 0.008 mM all-trans-3-hydroxyretinaldehyde Drosophila melanogaster
0.00027
-
 all-trans-retinal Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins Drosophila melanogaster
0.0004
-
 all-trans-retinal Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins Drosophila melanogaster
0.0006
-
 all-trans-retinal Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins Drosophila melanogaster
0.0007
-
 all-trans-retinal Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins Drosophila melanogaster
0.0032
-
 all-trans-3-hydroxyretinal Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins Drosophila melanogaster
0.0041
-
 all-trans-3-hydroxyretinal Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins Drosophila melanogaster
0.0044
-
 all-trans-3-hydroxyretinal Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated with microsomal membranes Drosophila melanogaster 16020
-

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q8MZG9
-
-
Drosophila melanogaster Q7JYX2
-
-
Drosophila melanogaster Q7JUS1
-
-
Drosophila melanogaster Q9W404
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans retinal + NADH + H+ prefers NADP+ and NADPH as cofactors Drosophila melanogaster all-trans-retinol + NAD+
-
 r
all-trans retinal + NADPH + H+ prefers NADP+ and NADPH as cofactors Drosophila melanogaster all-trans-retinol + NADP+
-
 r
all-trans-3-hydroxyretinal + NADH + H+ catalytic efficiency towards all-trans-3-hydroxyretinal is lower than that towards all-trans retinal, prefers NADP+ and NADPH as cofactors Drosophila melanogaster all-trans-3-hydroxyretinol + NAD+
-
 ?
all-trans-3-hydroxyretinal + NADPH + H+ catalytic efficiency towards all-trans-3-hydroxyretinal is lower than that towards all-trans retinal, prefers NADP+ and NADPH as cofactors Drosophila melanogaster all-trans-3-hydroxyretinol + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
CG2065
-
 Drosophila melanogaster
CG2070
-
 Drosophila melanogaster
CG30491
-
 Drosophila melanogaster
CG3842
-
 Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
NAD+ prefers NADP+ and NADPH as cofactors Drosophila melanogaster
NADH prefers NADP+ and NADPH as cofactors Drosophila melanogaster
NADP+ prefers NADP+ and NADPH as cofactors Drosophila melanogaster
NADPH prefers NADP+ and NADPH as cofactors Drosophila melanogaster