Cloned (Comment) | Organism |
---|---|
gene qsuD, recombinant expression in Escherichia coli | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a gene qsuD deletion mutant. Cell cultures of the qsuD-deficient strain in glucose-containing medium are light yellow, whereas those of the wild-type in shikimate or quinate-containing medium are brown | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis constant with quinate as substrate is lower than that with shikimate under optimal conditions | Corynebacterium glutamicum | |
0.42 | - |
3-dehydroquinate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH | Corynebacterium glutamicum | |
0.444 | - |
L-quinate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ | Corynebacterium glutamicum | |
1.141 | - |
3-dehydroquinate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADPH | Corynebacterium glutamicum | |
1.299 | - |
shikimate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ | Corynebacterium glutamicum | |
5.933 | - |
3-dehydroshikimate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
- |
Corynebacterium glutamicum |
60000 | - |
about, gel filtration | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NAD+ | Corynebacterium glutamicum | - |
3-dehydroquinate + NADH + H+ | - |
? | |
shikimate + NAD+ | Corynebacterium glutamicum | - |
3-dehydroshikimate + NADH + H+ | - |
? | |
shikimate + NAD+ | Corynebacterium glutamicum JCM 18229 | - |
3-dehydroshikimate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | A4QB65 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NAD+ | - |
Corynebacterium glutamicum | 3-dehydroquinate + NADH + H+ | - |
? | |
additional information | QsuD reduces 3-dehydroquinate using NADH and oxidizes quinate using NAD+ as cofactor | Corynebacterium glutamicum | ? | - |
? | |
additional information | QsuD reduces 3-dehydroquinate using NADH and oxidizes quinate using NAD+ as cofactor | Corynebacterium glutamicum JCM 18229 | ? | - |
? | |
shikimate + NAD+ | - |
Corynebacterium glutamicum | 3-dehydroshikimate + NADH + H+ | - |
? | |
shikimate + NAD+ | - |
Corynebacterium glutamicum JCM 18229 | 3-dehydroshikimate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 30000, about, recombinant enzyme, SDS-PAGE | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
cgR_0495 | - |
Corynebacterium glutamicum |
qsuD | - |
Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.75 | - |
shikimate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ | Corynebacterium glutamicum | |
5.67 | - |
3-dehydroshikimate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH | Corynebacterium glutamicum | |
9.31 | - |
3-dehydroquinate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADPH | Corynebacterium glutamicum | |
14.6 | - |
L-quinate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ | Corynebacterium glutamicum | |
114 | - |
3-dehydroquinate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of 3-dehydroquinate with NADH | Corynebacterium glutamicum |
8.5 | - |
oxidation of quinate with NAD+ | Corynebacterium glutamicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the catalytic reaction of QsuD is highly susceptible to pH | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the turnover number is lower with NADP+ instead of NAD+ as cofactor. NADP(H) is just only for 3-dehydroquinate reduction by the enzyme | Corynebacterium glutamicum | |
NAD+ | - |
Corynebacterium glutamicum | |
NADH | - |
Corynebacterium glutamicum |
Organism | Comment | Expression |
---|---|---|
Corynebacterium glutamicum | qsuD mRNA expression is upregulated in shikimate-grown cells relative to that in the glucose-grown cells | up |
General Information | Comment | Organism |
---|---|---|
physiological function | QsuD is essential for growth on shikimate and quinate as sole carbon sources, suggesting that it is the key enzyme for shikimate/quinate utilization | Corynebacterium glutamicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.96 | - |
3-dehydroshikimate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH | Corynebacterium glutamicum | |
1.35 | - |
shikimate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ | Corynebacterium glutamicum | |
8.16 | - |
3-dehydroquinate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADPH | Corynebacterium glutamicum | |
32.88 | - |
L-quinate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ | Corynebacterium glutamicum | |
271.4 | - |
3-dehydroquinate | pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH | Corynebacterium glutamicum |