Literature summary for 1.1.1.28 extracted from

Lawton, J.A.; Prescott, N.A.; Lawton, P.X.
From gene to structure Lactobacillus bulgaricus D-lactate dehydrogenase from yogurt as an integrated curriculum model for undergraduate molecular biology and biochemistry laboratory courses (2018), Biochem. Mol. Biol. Educ., 46, 270-278 .

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Application

Application	Comment	Organism
additional information	protocol for undergraduate molecular biology and biochemistry laboratory courses spanning two semesters that is organized around the LdhA gene from the yogurt-fermenting bacterium Lactobacillus bulgaricus, using commercially available yogurt. Curriculum starts with cloning the LdhA gene into a prokaryotic expression vector, followed by mRNA isolation and characterization of recombinant gene expression levels using RT-PCR. The biochemistry module begins with overexpression of the cloned LdhA gene and guides students through the process of affinity purification, biochemical characterization of the purified LdhA protein, and analysis of enzyme kinetics using various substrates and an inhibitor, concluding with a guided inquiry investigation of structure-function relationships in the three-dimensional structure of LdhA using molecular visualization software	Lactobacillus delbrueckii subsp. bulgaricus

Organism

Organism	UniProt	Comment	Textmining
Lactobacillus delbrueckii subsp. bulgaricus	-	-	-

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Release 2023.1 (January 2023)