Cloned (Comment) | Organism |
---|---|
gene fx, expression of the N-terminally His6-tagged enzyme in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapor diffusion method, 5 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 100 mM NaCl, is mixed with well solution containing 100 mM Tris, pH 8.0, 30% PEG monomethyl ether 2000, X-ray diffraction structure determination and analysis at 2.37 A resolution, molecular replacement | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene fx | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+ | the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4 | Homo sapiens | GDP-beta-L-fucose + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | two homodimers, each subunit consists of two domains, a Rossmann-fold NADPH-binding motif and a carboxyl terminal domain, quaternary structure and intermolecular contacts, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
FX protein | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on, binding site structure, overview | Homo sapiens |