Application | Comment | Organism |
---|---|---|
synthesis | coexpression of enzyme and glucose dehydrogenase gene in Escherichia coli efficiently reduces 3,4-dihydroxyphenylpyruvate to L-3,4-dihydroxyphenyllactate with 95.45% isolation yield | Limosilactobacillus fermentum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 6 h, 60% decrease in activity | Limosilactobacillus fermentum | |
Fe2+ | 6 h, 70% decrease in activity | Limosilactobacillus fermentum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.123 | - |
NADH | pH 6, 25°C | Limosilactobacillus fermentum | |
0.197 | - |
NADPH | pH 6, 25°C | Limosilactobacillus fermentum | |
2.47 | - |
pyruvate | pH 6, 25°C | Limosilactobacillus fermentum | |
11.37 | - |
3,4-Dihydroxyphenylpyruvate | pH 6, 25°C | Limosilactobacillus fermentum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 6 h, 20% increase in activity | Limosilactobacillus fermentum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus fermentum | A0A2K9Y5T7 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.48 | - |
substrate 3,4-dihydroxyphenylpyruvate, pH 6, 25°C | Limosilactobacillus fermentum |
144.8 | - |
substrate pyruvate, pH 6, 25°C | Limosilactobacillus fermentum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + NADH + H+ | 11.5% of the activity with pyruvate | Limosilactobacillus fermentum | 2-hydroxybutyrate + NAD+ | - |
? | |
2-oxoglutarate + NADH + H+ | 14.6% of the activity with pyruvate | Limosilactobacillus fermentum | 2-hydroxyglutarate + NAD+ | - |
? | |
3,4-dihydroxyphenylpyruvate + NADH + H+ | 8.5% of the activity with pyruvate | Limosilactobacillus fermentum | L-3,4-dihydroxyphenyllactate + NAD+ | - |
? | |
4-hydroxyphenylpyruvate + NADH + H+ | 11.1% of the activity with pyruvate | Limosilactobacillus fermentum | L-4-hydroxyphenyllactate + NAD+ | - |
? | |
4-methyl-2-oxopentanoate + NADH + H+ | 25.9% of the activity with pyruvate | Limosilactobacillus fermentum | 2-hydroxy-4-methylpentanoate + NAD+ | - |
? | |
benzoylformate + NADH + H+ | 20.5% of the activity with pyruvate | Limosilactobacillus fermentum | L-hydroxy(phenyl)acetate + NAD+ | - |
? | |
phenylpyruvate + NADH + H+ | 47.4% of the activity with pyruvate | Limosilactobacillus fermentum | L-phenyllactate + NAD+ | - |
? | |
pyruvate + NADPH + H+ | - |
Limosilactobacillus fermentum | L-lactate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33650, calcuated from sequence | Limosilactobacillus fermentum |
Synonyms | Comment | Organism |
---|---|---|
L-Ldh0845 | - |
Limosilactobacillus fermentum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Limosilactobacillus fermentum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.293 | - |
3,4-Dihydroxyphenylpyruvate | pH 6, 25°C | Limosilactobacillus fermentum | |
10.37 | - |
NADPH | pH 6, 25°C | Limosilactobacillus fermentum | |
12.45 | - |
pyruvate | pH 6, 25°C | Limosilactobacillus fermentum | |
64.42 | - |
NADH | pH 6, 25°C | Limosilactobacillus fermentum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Limosilactobacillus fermentum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
stable | Limosilactobacillus fermentum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADH is 10fold preferred over NADPH | Limosilactobacillus fermentum | |
NADPH | NADH is 10fold preferred over NADPH | Limosilactobacillus fermentum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0258 | - |
3,4-Dihydroxyphenylpyruvate | pH 6, 25°C | Limosilactobacillus fermentum | |
5.05 | - |
pyruvate | pH 6, 25°C | Limosilactobacillus fermentum | |
52.52 | - |
NADPH | pH 6, 25°C | Limosilactobacillus fermentum | |
521.9 | - |
NADH | pH 6, 25°C | Limosilactobacillus fermentum |