Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
mutant A104L, structure reveals a reduced cavity C-1 volume. The allosteric switch still adopts the E and E* states, albeit with a more rigid protein core | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A104L | ubstitution introduced to fill a cavity in the E state and sterically prevent repacking of the core into the inactive Eomega state. Mutant A104L does not show hysteresis or negative cooperativity, binds UDP-xylose with lower affinity and the inhibition is no longer cooperative | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0076 | - |
UDP-alpha-D-glucose | wild-type, K0.5 value, Hill coefficient 1, pH 7.5, 25°C | Homo sapiens | |
0.0094 | - |
UDP-alpha-D-glucose | mutant A104L, K0.5 value, Hill coefficient 1, pH 7.5, 25°C | Homo sapiens | |
0.048 | - |
NAD+ | mutant A104L, K0.5 value, Hill coefficient 1, pH 7.5, 25°C | Homo sapiens | |
1.16 | - |
NAD+ | wild-type, K0.5 value, Hill coefficient 0.69, pH 7.5, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60701 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + 2 NAD+ + H2O | - |
Homo sapiens | UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
UDP-alpha-D-glucose | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.79 | - |
NAD+ | mutant A104L, pH 7.5, 25°C | Homo sapiens | |
0.82 | - |
UDP-alpha-D-glucose | mutant A104L, pH 7.5, 25°C | Homo sapiens | |
0.94 | - |
NAD+ | wild-type, pH 7.5, 25°C | Homo sapiens |