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Literature summary for 1.1.1.213 extracted from

  • Nakamura, S.; Oda, M.; Kataoka, S.; Ueda, S.; Uchiyama, S.; Yoshida, T.; Kobayashi, Y.; Ohkubo, T.
    Apo- and holo-structures of 3alpha-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831. Loop-helix transition induced by coenzyme binding (2006), J. Biol. Chem., 281, 31876-31884.
    View publication on PubMed
Search for more literature for 1.1.1.213

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme with NADH, hanging drop vapour diffusion method, 4°C, in presence of 1.4 M ammonium sulfate, 0.14 M NaCl, and 0.1 M Tris, pH 9.0, rod cluster crystals appear within 1 week, X-ray diffraction structure determination and analysis at 1.8 A resolution, modeling Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5alpha-dihydrotestosterone + NAD(P)H + H+ Pseudomonas sp.
-
 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Pseudomonas sp. 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 26000 Pseudomonas sp.
additional information comparison of the folding topologies found in the crystal structures of the holo-enzyme and the apo-enzyme, structural changes in solution, overview Pseudomonas sp.

Synonyms

Synonyms Comment Organism
3alpha-hydroxysteroid dehydrogenase
-
 Pseudomonas sp.
3alphaHSD
-
 Pseudomonas sp.
additional information cf. EC 1.1.1.50, the enzyme belongs to the AKR1C family Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD(P)H cofactor binding structure, overview Pseudomonas sp.