Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermoanaerobacterium saccharolyticum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Thermoanaerobacterium saccharolyticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium saccharolyticum | - |
- |
- |
Thermoanaerobacterium saccharolyticum LL1049 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
AdhA appears to be insensitive to the presence of oxygen | Thermoanaerobacterium saccharolyticum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.8 | - |
pH 7.0, 55°C | Thermoanaerobacterium saccharolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADPH + H+ | - |
Thermoanaerobacterium saccharolyticum | ethanol + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Thermoanaerobacterium saccharolyticum LL1049 | ethanol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AdhA | - |
Thermoanaerobacterium saccharolyticum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | about 4% of the activity with NADPH | Thermoanaerobacterium saccharolyticum | |
NADPH | - |
Thermoanaerobacterium saccharolyticum |
General Information | Comment | Organism |
---|---|---|
physiological function | gene deletion of adhA reduces ethanol production by about 50%, whereas multiple gene deletions of all annotated alcohol dehydrogenases except adhA and adhE does not affect ethanol production. Deletion of adhA in wild-type reduces NADPH-linked ADH activity (acetaldehyde-reducing) by 93% | Thermoanaerobacterium saccharolyticum |