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Literature summary for 1.1.1.207 extracted from

  • Davis, E.M.; Ringer, K.L.; McConkey, M.E.; Croteau, R.
    Monoterpene metabolism. Cloning, expression, and characterization of menthone reductases from peppermint (2005), Plant Physiol., 137, 873-881.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli Mentha x piperita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00012
-
NADPH pH 7.0, 31┬░C, recombinant enzyme Mentha x piperita
0.003
-
(-)-menthone pH 7.0, 31┬░C, recombinant enzyme Mentha x piperita
0.041
-
(+)-isomenthone pH 7.0, 31┬░C, recombinant enzyme Mentha x piperita

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34070
-
x * 34070, amino acid sequence calculation Mentha x piperita

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-isomenthone + NADPH Mentha x piperita
-
(+)-neoisomenthol + NADP+
-
?
(-)-menthone + NADPH Mentha x piperita
-
(3R)-menthol + NADP+
-
?
additional information Mentha x piperita primary monoterpene biosynthesis pathway, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Mentha x piperita
-
cv. Black Mitchum
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme partially Mentha x piperita

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Mentha x piperita
-
oil gland secretory cell
-
Mentha x piperita
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-isomenthone + NADPH
-
Mentha x piperita (+)-neoisomenthol + NADP+
-
?
(-)-menthone + NADPH
-
Mentha x piperita (3R)-menthol + NADP+
-
?
(-)-menthone + NADPH
-
Mentha x piperita (3R)-menthol + NADP+ (3R)-menthol is the main product, product determination by gas chromatography, overview ir
additional information primary monoterpene biosynthesis pathway, overview Mentha x piperita ?
-
?
additional information monoterpenol is no substrate Mentha x piperita ?
-
?

Subunits

Subunits Comment Organism
? x * 34070, amino acid sequence calculation Mentha x piperita

Synonyms

Synonyms Comment Organism
(-)-menthone:(3R)-menthol reductase
-
Mentha x piperita
MMR
-
Mentha x piperita

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
31
-
assay at Mentha x piperita

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
NADPH pH 7.0, 31┬░C, recombinant enzyme Mentha x piperita
0.6
-
(-)-menthone pH 7.0, 31┬░C, recombinant enzyme Mentha x piperita

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Mentha x piperita

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 50% of maximal activity at pH 4.5 and pH 8.5 Mentha x piperita

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Mentha x piperita