KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
Tropine | - |
Pseudomonas sp. | |
0.048 | - |
NADP+ | - |
Pseudomonas sp. | |
0.073 | - |
nortropine | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Pseudomonas sp. |
58000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropine + NADP+ | Pseudomonas sp. | role in the pathway for tropine catabolism | tropinone + NADPH | - |
r | |
tropine + NADP+ | Pseudomonas sp. AT3 | role in the pathway for tropine catabolism | tropinone + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
AT3 | - |
Pseudomonas sp. AT3 | - |
AT3 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.316 | - |
crude extract | Pseudomonas sp. |
63.82 | - |
purified enzyme | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nortropine + NADP+ | - |
Pseudomonas sp. | nortropinone + NADPH | - |
? | |
nortropine + NADP+ | - |
Pseudomonas sp. AT3 | nortropinone + NADPH | - |
? | |
tropine + NADP+ | - |
Pseudomonas sp. | tropinone + NADPH | - |
r | |
tropine + NADP+ | role in the pathway for tropine catabolism | Pseudomonas sp. | tropinone + NADPH | - |
r | |
tropine + NADP+ | - |
Pseudomonas sp. AT3 | tropinone + NADPH | - |
r | |
tropine + NADP+ | role in the pathway for tropine catabolism | Pseudomonas sp. AT3 | tropinone + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 identical subunits, gel filtration | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
loss of 65% activity after 60 hours in crude extracts | Pseudomonas sp. |
4 | - |
loss of 90% activity of the purified enzyme after 10 hours, presence of 10% ethanol during the purification procedure | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NAD+ | Pseudomonas sp. | |
NADP+ | - |
Pseudomonas sp. |