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Literature summary for 1.1.1.203 extracted from

  • Wagschal, K.; Jordan, D.B.; Hart-Cooper, W.M.; Chan, V.J.
    Penicillium camemberti galacturonate reductase C-1 oxidation/reduction of uronic acids and substrate inhibition mitigation by aldonic acids (2020), Int. J. Biol. Macromol., 153, 1090-1098 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 (DE3) cells Penicillium camemberti

Inhibitors

Inhibitors Comment Organism Structure
galacturonate
-
Penicillium camemberti
glucuronate glucuronate shows minor substrate inhibition Penicillium camemberti
L-gulonate competitive inhibitor Penicillium camemberti
L-gulono-1,4-lactone competitive inhibitor Penicillium camemberti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
NADPH with D-glucuronic acid as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
0.4
-
NADH at pH 7.5 and 25┬░C Penicillium camemberti
0.95
-
D-galacturonic acid with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
3.15
-
D-galacturonic acid with NADH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
3.3
-
L-galactonate with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
3.34
-
D-glucuronic acid with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
4.7
-
L-Gulonic acid with NADP+ as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
6.3
-
D-glucuronic acid with NADH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
7.1
-
D-glucurono-3,6-lactone with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
8.1
-
L-galactono-1,4-lactone with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
9.5
-
L-gulono-1,4-lactone with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galacturonic acid + NADPH Penicillium camemberti
-
L-galactonate + NADP+
-
?
D-glucuronic acid + NADPH Penicillium camemberti
-
L-gulonic acid + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Penicillium camemberti
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Penicillium camemberti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonic acid + NADPH
-
Penicillium camemberti L-galactonate + NADP+
-
?
D-glucuronic acid + NADH
-
Penicillium camemberti L-gulonic acid + NAD+
-
?
D-glucuronic acid + NADPH
-
Penicillium camemberti L-gulonic acid + NADP+
-
?
D-glucurono-3,6-lactone + NADPH + H+
-
Penicillium camemberti L-gulono-1,4-lactone + NADP+
-
?
L-galactonate + NADP+
-
Penicillium camemberti D-galacturonic acid + NADPH
-
?
L-galactono-1,4-lactone + NADPH
-
Penicillium camemberti ?
-
?
L-gulonic acid + NAD+
-
Penicillium camemberti D-glucuronic acid + NADH
-
?
L-gulono-1,4-lactone + NADPH
-
Penicillium camemberti ?
-
?

Synonyms

Synonyms Comment Organism
D-GalUA oxidoreductase
-
Penicillium camemberti
galacturonate oxidoreductase
-
Penicillium camemberti
Gor
-
Penicillium camemberti

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
37 40 the enzyme has low thermal stability, with at KT0.5 of 37┬░C, and complete unfolding after 1 h incubation at 40┬░C Penicillium camemberti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.41
-
L-galactono-1,4-lactone with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
0.41
-
L-gulono-1,4-lactone with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
0.75
-
L-galactonate with NADP+ as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
0.97
-
L-Gulonic acid with NADP+ as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
2.3
-
D-glucurono-3,6-lactone with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
7.4
-
NADH at pH 7.5 and 25┬░C Penicillium camemberti
7.6
-
D-glucuronic acid with NADH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
9.6
-
NADPH with D-glucuronic acid as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
13
-
D-glucuronic acid with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
13.6
-
D-galacturonic acid with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
18.5
-
D-galacturonic acid with NADH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Penicillium camemberti

pH Range

pH Minimum pH Maximum Comment Organism
6 9 more than 50% activity between pH 6.0 and 9.0 Penicillium camemberti

Cofactor

Cofactor Comment Organism Structure
NADH
-
Penicillium camemberti
NADPH preferred cofactor Penicillium camemberti

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.8
-
L-gulonate at pH 7.5 and 25┬░C Penicillium camemberti
7.2
-
L-gulono-1,4-lactone at pH 7.5 and 25┬░C Penicillium camemberti

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
L-gulono-1,4-lactone with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
0.05
-
L-galactono-1,4-lactone with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
0.2
-
L-Gulonic acid with NADP+ as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
0.23
-
L-galactonate with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
0.32
-
D-glucurono-3,6-lactone with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
1.2
-
D-glucuronic acid with NADH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
3.89
-
D-glucuronic acid with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
5.87
-
D-galacturonic acid with NADH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
14.3
-
D-galacturonic acid with NADPH as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti
19
-
NADH at pH 7.5 and 25┬░C Penicillium camemberti
1655
-
NADPH with D-glucuronic acid as cosubstrate, at pH 7.5 and 25┬░C Penicillium camemberti