Cloned (Comment) | Organism |
---|---|
expression in Clostridium acetobutylicum mutant strain DG1 from plasmid pSPD5, subcloning in Escherichia coli | Clostridium butyricum |
Protein Variants | Comment | Organism |
---|---|---|
nore | expression of the Clostridumm butyricum enzyme in Clostridium acetobutylicum strain DG1(pSPD5) leads to functional reduction of glycerol to 1,3-propanediol in a two-step process, the first step of glycerol reduction to 3-hydroxypropionaldehyde, which is then reduced to 1,3-propanediol, is performed by the B12-independent glycerol dehydratase | Clostridium butyricum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NADH | Clostridium butyricum | - |
propane-1,3-diol + NAD+ | - |
? | |
glycerol + NADH | Clostridium butyricum VPI 3266 | - |
propane-1,3-diol + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium butyricum | - |
- |
- |
Clostridium butyricum VPI 3266 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
nucleotide pools of wild-type Clostridium butyricum strain VPI 3266 and recombinant Clostridium acetobutylicum mutant strain DG1 | Clostridium butyricum |
0.37 | - |
wild-type Clostridium butyricum strain VPI 3266 | Clostridium butyricum |
1.2 | - |
recombinant enzyme in Clostridium acetobutylicum mutant strain DG1 | Clostridium butyricum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxypropionaldehyde + NADH | recombinant mutant Clostridium acetobutylicum strain DG1(pSPD5) expressing the enzyme from an introduced plasmid | Clostridium butyricum | propane-1,3-diol + NAD+ | - |
? | |
3-hydroxypropionaldehyde + NADH | recombinant mutant Clostridium acetobutylicum strain DG1(pSPD5) expressing the enzyme from an introduced plasmid | Clostridium butyricum VPI 3266 | propane-1,3-diol + NAD+ | - |
? | |
glycerol + NADH | - |
Clostridium butyricum | propane-1,3-diol + NAD+ | - |
? | |
glycerol + NADH | - |
Clostridium butyricum VPI 3266 | propane-1,3-diol + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,3-Pdiol dehydrogenase | - |
Clostridium butyricum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Clostridium butyricum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Clostridium butyricum |