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Literature summary for 1.1.1.2 extracted from

  • Inokuma, K.; Nakashimada, Y.; Akahoshi, T.; Nishio, N.
    Characterization of enzymes involved in the ethanol production of Moorella sp. HUC22-1 (2007), Arch. Microbiol., 188, 37-45.
    View publication on PubMed
Search for more literature for 1.1.1.2

Application

Application Comment Organism
synthesis 50 microg of alcohol dehydrogenase AdhA, EC 1.1.1.2, and 50 microg actaldehyde dehydrogenase AldH, EC 1.2.1.10,in buffer solution (pH 8.0) containing NADPH, NADH and acetyl-CoA at 60°C, produce 1.6 mM ethanol from 3 mM acetyl-CoA after 90 min Moorella sp. HUC22-1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Moorella sp. HUC22-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
 acetaldehyde isoform AdhA, pH 8.0, 60°C Moorella sp. HUC22-1
40
-
 ethanol isoform AdhA, pH 8.0, 60°C Moorella sp. HUC22-1
55.8
-
 acetaldehyde isoform AdhB, pH 8.0, 60°C Moorella sp. HUC22-1

Organism

Organism UniProt Comment Textmining
Moorella sp. HUC22-1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.032
-
 isoform AdhA, oxidation of ethanol, cofactor NAD+, pH 8.0, 60°C Moorella sp. HUC22-1
0.049
-
 isoform AdhA, reduction of acetaldehyde, cofactor NADH, pH 8.0, 60°C Moorella sp. HUC22-1
2.9
-
 isoform AdhB, reduction of acetaldehyde, cofactor NADPH, pH 8.0, 60°C Moorella sp. HUC22-1
11.9
-
 isoform AdhA, oxidation of ethanol, cofactor NADP+, pH 8.0, 60°C Moorella sp. HUC22-1
50.4
-
 isoform AdhA, reduction of acetaldehyde, cofactor NADPH, pH 8.0, 60°C Moorella sp. HUC22-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NADPH + H+
-
 Moorella sp. HUC22-1 ethanol + NADP+
-
 r
butan-1-ol + NADP+ isoform AdhA, 238% of the activity with acetaldehyde Moorella sp. HUC22-1 butanal + NADPH + H+
-
 r
butanal + NADPH + H+ isoform AdhA, 625%, isoform AdhB, 108% of the activity with acetaldehyde Moorella sp. HUC22-1 butan-1-ol + NADP+
-
 r
ethanol + NADP+ isoform AdhB: no oxidation of ethanol Moorella sp. HUC22-1 acetaldehyde + NADPH + H+
-
 r
isobutanol + NADP+ isoform AdhA, 221% of the activity with acetaldehyde Moorella sp. HUC22-1 isobutyl aldehyde + NADPH + H+
-
 r
isobutyl aldehyde + NADPH + H+ isoform AdhA, 469%, isoform AdhB, 48% of the activity with acetaldehyde Moorella sp. HUC22-1 isobutanol + NADP+
-
 r
propan-1-ol + NADP+ isoform AdhA, 162% of the activity with acetaldehyde Moorella sp. HUC22-1 propanal + NADPH + H+
-
 r
propanal + NADPH + H+ isoform AdhA, 50%, isoform AdhB, 62% of the activity with acetaldehyde Moorella sp. HUC22-1 propan-1-ol + NADP+
-
 r

Synonyms

Synonyms Comment Organism
AdhA
-
 Moorella sp. HUC22-1
AdhB
-
 Moorella sp. HUC22-1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 at pH 8.0 Moorella sp. HUC22-1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 isoform AdhA, 84%, isoform AdhB, 26% of maximum activity Moorella sp. HUC22-1
70
-
 isoform AdhA, 61%, isoform AdhB 64% of maximum activity Moorella sp. HUC22-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 reduction of acetaldehyde Moorella sp. HUC22-1
10
-
 oxidation of ethanol Moorella sp. HUC22-1

Cofactor

Cofactor Comment Organism Structure
additional information isoform AdhA, less than 1% activity with NAD(H), isoform AdhB, no activity with NAD(H) Moorella sp. HUC22-1
NADPH
-
 Moorella sp. HUC22-1