General Stability | Organism |
---|---|
high concentration of salt, essential to maintain the enzymatic activity and structural integrity of the halophilic enzyme under standard conditions may be partially replaced by DMSO and MeOH | Haloferax volcanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | high concentration of salt, essential to maintain the enzymatic activity and structural integrity of the halophilic enzyme under standard conditions may be partially replaced by DMSO and MeOH | Haloferax volcanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + NADP+ | Haloferax volcanii | - |
acetaldehyde + NADPH + H+ | - |
r |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 62% remaining activity at 10% after 72 h at 5°C, 34% at 5°C, rapid inactivation at 50°C and 30% | Haloferax volcanii |
DMSO | 78% remaining activity at 30% after 72 h at 5°C, inactivation within 24 h at 50°C and 30% | Haloferax volcanii |
Methanol | at 5°C and 50°C, 38% remaining activity at 30%, 67% at 10%, and 70% at 5% after 72 h, inactivation within 24 h at 50°C and 30% | Haloferax volcanii |
additional information | longterm effects of organic solvents at 5°C and 50°C, overview. High concentration of salt, essential to maintain the enzymatic activity and structural integrity of the halophilic enzyme under standard conditions may be partially replaced by DMSO and MeOH. Presence of organic solvents does not induce gross changes in substrate specificity | Haloferax volcanii |
tetrahydrofuran | at 5°C, 32% activity remaining after 24 h, 7% after 72 h at 5% THF, 12% remaining activity at 10% THF after 24 h, inactivation within 72 h at 10% THF, and within 24 h at 30%THF | Haloferax volcanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + NADP+ | - |
Haloferax volcanii | acetaldehyde + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ADH2 | - |
Haloferax volcanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Haloferax volcanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
oxidative reaction | Haloferax volcanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Haloferax volcanii | |
NADPH | - |
Haloferax volcanii |