Cloned (Comment) | Organism |
---|---|
gene CAD or HP1140 from strain 26995, cloning in Escherichia coli strain DH5alpha, and expression in strain BL21(DE3) as N-terminally His-tagged enzyme | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyl alcohol | competitive mechanism, substrate inhibition | Helicobacter pylori | |
cinnamyl alcohol | competitive mechanism, substrate inhibition | Helicobacter pylori | |
coniferyl alcohol | competitive mechanism, substrate inhibition | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
cinnamyl aldehyde | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
0.008 | - |
coniferyl aldehyde | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
0.03 | - |
benzaldehyde | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
0.04 | - |
acetaldehyde | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
0.06 | - |
NADP+ | pH 7.5, 37°C, recombinant enzyme, with benzyla alcohol | Helicobacter pylori | |
0.1 | - |
cinnamyl alcohol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
0.11 | - |
coniferyl alcohol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
0.15 | - |
NADPH | pH 7.5, 37°C, recombinant enzyme, with acetaldehyde | Helicobacter pylori | |
0.41 | - |
benzyl alcohol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
9 | - |
butanol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
13 | - |
Propanol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
31 | - |
benzaldehyde | pH 7.5, 37°C, recombinant enzyme, dismutase reaction with NADP+ | Helicobacter pylori | |
46 | - |
ethanol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | predominantly | Helicobacter pylori | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamyl alcohol + NADP+ | Helicobacter pylori | - |
cinnamyl aldehyde + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
strains 1061, 26695, and G27, gene CAD | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Helicobacter pylori |
Storage Stability | Organism |
---|---|
-20°C, purified recombinant His-tagged enzyme, 75 mM sodium phosphate, pH 7.5, 5 mM DTT, no loss of activity after 1 month | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NADP+ | the enzyme also shows dismutase activity | Helicobacter pylori | benzoic acid + NADPH | - |
? | |
benzyl alcohol + NADP+ | - |
Helicobacter pylori | benzaldehyde + NADPH | - |
r | |
butanol + NADP+ | - |
Helicobacter pylori | butyraldehyde + NADPH | - |
r | |
cinnamyl alcohol + NADP+ | - |
Helicobacter pylori | cinnamyl aldehyde + NADPH | - |
r | |
coniferyl alcohol + NADP+ | - |
Helicobacter pylori | coniferyl aldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Helicobacter pylori | acetaldehyde + NADPH + H+ | - |
r | |
propanol + NADP+ | - |
Helicobacter pylori | propionaldehyde + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CAD | - |
Helicobacter pylori |
cinnamyl alcohol dehydrogenase | - |
Helicobacter pylori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
benzaldehyde | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
2.5 | - |
benzaldehyde | pH 7.5, 37°C, recombinant enzyme, dismutase reaction with NADP+ | Helicobacter pylori | |
3.5 | - |
coniferyl alcohol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
5.7 | - |
butanol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
7.1 | - |
ethanol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
7.7 | - |
cinnamyl aldehyde | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
8.4 | - |
benzyl alcohol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
12.8 | - |
Propanol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
13.3 | - |
cinnamyl alcohol | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
15.5 | - |
NADP+ | pH 7.5, 37°C, recombinant enzyme, with benzyla alcohol | Helicobacter pylori | |
16.7 | - |
acetaldehyde | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori | |
25.2 | - |
NADPH | pH 7.5, 37°C, recombinant enzyme, with acetaldehyde | Helicobacter pylori | |
27.4 | - |
coniferyl aldehyde | pH 7.5, 37°C, recombinant enzyme | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Helicobacter pylori | |
NADPH | - |
Helicobacter pylori |