Cloned (Comment) | Organism |
---|---|
gene mtlD, transcriptional organization of the gene cluster, quantitative RT-PCR enzyme expression analysis | Bacillus methanolicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | functional complementation of arabitol dehydrogenase activity of gene MtlD in Corynebacterium glutamicum DELTAmtlD deletion mutant by gene atlD of Bacillus methanolicus strain MG3 | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitol 1-phosphate + NAD+ | Bacillus methanolicus | - |
D-xylulose 5-phosphate + NADH + H+ | - |
r | |
D-arabitol 1-phosphate + NAD+ | Bacillus methanolicus ATCC 53907 | - |
D-xylulose 5-phosphate + NADH + H+ | - |
r | |
D-arabitol 1-phosphate + NAD+ | Bacillus methanolicus MGA3 | - |
D-xylulose 5-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | Bacillus methanolicus | - |
D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | Corynebacterium glutamicum | - |
D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | Bacillus methanolicus ATCC 53907 | - |
D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | Bacillus methanolicus MGA3 | - |
D-fructose 6-phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | I3E3X0 | - |
- |
Bacillus methanolicus ATCC 53907 | I3E3X0 | - |
- |
Bacillus methanolicus MGA3 | I3E3X0 | - |
- |
Corynebacterium glutamicum | A0A0U4Y4V3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | growth of Bacillus methanolicus strain MGA3 on arabitol as single carbon source and as co-substrate to mannitol. Although arabitol supports growth of strain MGA3 as a sole source of carbon and energy, the strain grows at a lower growth rate than with its preferred sugar alcohol substrate mannitol. Mannitol is utilized faster than arabitol and co-consumption of both sugar alcohols is observed, overview | Bacillus methanolicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
arabitol phosphate dehydrogenase activity in mannitol grown cells of strains MG3, pH 7.2, 30°C | Bacillus methanolicus |
0.05 | - |
arabitol phosphate dehydrogenase activity in arabitol grown cells of strains MG3, pH 7.2, 30°C | Bacillus methanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitol 1-phosphate + NAD+ | - |
Bacillus methanolicus | D-xylulose 5-phosphate + NADH + H+ | - |
r | |
D-arabitol 1-phosphate + NAD+ | - |
Corynebacterium glutamicum | D-xylulose 5-phosphate + NADH + H+ | - |
r | |
D-arabitol 1-phosphate + NAD+ | - |
Bacillus methanolicus ATCC 53907 | D-xylulose 5-phosphate + NADH + H+ | - |
r | |
D-arabitol 1-phosphate + NAD+ | - |
Bacillus methanolicus MGA3 | D-xylulose 5-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | - |
Bacillus methanolicus | D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | - |
Corynebacterium glutamicum | D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | - |
Bacillus methanolicus ATCC 53907 | D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | - |
Bacillus methanolicus MGA3 | D-fructose 6-phosphate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BMMGA3_RS01080 | locus name | Bacillus methanolicus |
More | cf. EC 1.1.1.301 | Bacillus methanolicus |
MtlD | - |
Bacillus methanolicus |
MtlD | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacillus methanolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Bacillus methanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus methanolicus | |
NAD+ | - |
Corynebacterium glutamicum | |
NADH | - |
Bacillus methanolicus | |
NADH | - |
Corynebacterium glutamicum |
Organism | Comment | Expression |
---|---|---|
Bacillus methanolicus | in Bacillus methanolicus, RNA levels for atlD are higher while lower for mtlD during growth with both carbon sources as compared to growth with mannitol alone | down |
Corynebacterium glutamicum | in Corynebacterium glutamicum, arabitol induces expression of genes rbtT, mtlD, sixA, xylB and atlR, and glucose represses expression of mtlD | down |
Bacillus methanolicus | differential gene expression analysis of Bacillus methanolicus strain MGA3 cells grown on arabitol as compared to mannitol via transcriptome sequencing (RNA-seq), quantitative RT-PCR expression analysis, the enzyme can be induced by arabitol | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | the Corynebacterium glutamicum arabitol-negative DELTAmtlD deletion mutant can be complemented by heterologous expression of Bacillus methanolicus strain MGA3 operon atlABCD or gene atlD | Corynebacterium glutamicum |
metabolism | the enzyme is involved in the arabitol catabolism and the pentose phosphate pathway, model of the pathways for arabitol catabolism in bacteria, overview | Bacillus methanolicus |
physiological function | Corynebacterium glutamicum is a natural D-arabitol utilizer that requires arabitol-utilizing mannitol-1-phosphate 5-dehydrogenase MtlD for arabitol catabolism | Corynebacterium glutamicum |