Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | weak inhibition | Escherichia coli | |
2,2'-bipyridyl | weak inhibition | Escherichia coli | |
4,7-phenanthroline | weak inhibition | Escherichia coli | |
5,5'-dithiobis-(2-nitrobenzoate) | complete inactivation at 0.24 mM | Escherichia coli | |
diethyldithiocarbamate | 95% inactivation, prevented by presence of 2-mercaptoethanol | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Escherichia coli | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 1.14 Zn atoms/monomer | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22500 | - |
2 * 22500, SDS-PAGE | Escherichia coli |
45500 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | Escherichia coli | - |
D-fructose 6-phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
502 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 0.03 M imidazole-HCl, pH 7.3, several months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | - |
Escherichia coli | D-fructose 6-phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 22500, SDS-PAGE | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NADH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
1,10-phenanthroline | - |
Escherichia coli | |
11 | - |
2,2'-bipyridyl | - |
Escherichia coli | |
12 | - |
4,7-phenanthroline | - |
Escherichia coli |