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Literature summary for 1.1.1.168 extracted from

  • Wang, J.; Cheng, P.; Wu, Y.; Wang, A.; Liu, F.; Pei, X.
    Discovery of a new NADPH-dependent aldo-keto reductase from Candida orthopsilosis catalyzing the stereospecific synthesis of (R)-pantolactone by genome mining (2019), J. Biotechnol., 291, 26-34 .
    View publication on PubMed
Search for more literature for 1.1.1.168

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Candida orthopsilosis

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Candida orthopsilosis
Co2+ 81.9% residual activity at 1 mM Candida orthopsilosis
Cu2+ 67.8% residual activity at 1 mM Candida orthopsilosis
EDTA
-
 Candida orthopsilosis
Fe2+ 81% residual activity at 1 mM Candida orthopsilosis
Mg2+ 80.2% residual activity at 1 mM Candida orthopsilosis
Mn2+ 67.6% residual activity at 1 mM Candida orthopsilosis
Pb2+ complete inhibition at 1 mM Candida orthopsilosis
Zn2+ 71.7% residual activity at 1 mM Candida orthopsilosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 2-dehydropantolactone at pH 7.0 and 30°C Candida orthopsilosis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information 1 mM Ni2+ and Ca2+ have almost no influence over the enzyme activity Candida orthopsilosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydropantolactone + NADPH + H+ Candida orthopsilosis
-
 (R)-pantolactone + NADP+
-
 ?
2-dehydropantolactone + NADPH + H+ Candida orthopsilosis 90-125
-
 (R)-pantolactone + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Candida orthopsilosis H8XA83
-
-
Candida orthopsilosis 90-125 H8XA83
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Candida orthopsilosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydropantolactone + NADPH + H+
-
 Candida orthopsilosis (R)-pantolactone + NADP+
-
 ?
2-dehydropantolactone + NADPH + H+ 100% activity, 99% conversion and more than 99% enantiomeric excess within 5 h Candida orthopsilosis (R)-pantolactone + NADP+
-
 ?
2-dehydropantolactone + NADPH + H+
-
 Candida orthopsilosis 90-125 (R)-pantolactone + NADP+
-
 ?
2-dehydropantolactone + NADPH + H+ 100% activity, 99% conversion and more than 99% enantiomeric excess within 5 h Candida orthopsilosis 90-125 (R)-pantolactone + NADP+
-
 ?
ethyl 2-oxopropanoate + NADPH + H+ 0.496% activity compared to 2-dehydropantolactone Candida orthopsilosis ethyl (2R)-2-hydroxypropanoate + NADP+
-
 ?
ethyl oxo(phenyl)acetate + NADPH + H+ 3.45% activity compared to 2-dehydropantolactone Candida orthopsilosis ethyl (2R)-hydroxy(phenyl)acetate + NADP+
-
 ?
methyl 2-oxopropanoate + NADPH + H+ 0.501% activity compared to 2-dehydropantolactone Candida orthopsilosis methyl (2R)-2-hydroxypropanoate + NADP+
-
 ?
methyl 2-oxopropanoate + NADPH + H+ 0.501% activity compared to 2-dehydropantolactone Candida orthopsilosis 90-125 methyl (2R)-2-hydroxypropanoate + NADP+
-
 ?
methyl oxo(phenyl)acetate + NADPH + H+ 3.94% activity compared to 2-dehydropantolactone Candida orthopsilosis methyl (2R)-hydroxy(phenyl)acetate + NADP+
-
 ?
additional information the enzyme does not reduce 4-nitrobenzaldehyde Candida orthopsilosis ?
-
 ?
additional information the enzyme does not reduce 4-nitrobenzaldehyde Candida orthopsilosis 90-125 ?
-
 ?
pyridine 3-aldehyde + NADPH + H+ 0.538% activity compared to 2-dehydropantolactone Candida orthopsilosis (pyridin-3-yl)methanol + NADP+
-
 ?
pyridine 3-aldehyde + NADPH + H+ 0.538% activity compared to 2-dehydropantolactone Candida orthopsilosis 90-125 (pyridin-3-yl)methanol + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, His6-tagged enzyme, SDS-PAGE Candida orthopsilosis

Synonyms

Synonyms Comment Organism
AKR
-
 Candida orthopsilosis
NADPH-dependent aldo-keto reductase
-
 Candida orthopsilosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Candida orthopsilosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 the activity remains above 50% of the maximum activity between 30 and 55°C Candida orthopsilosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 the recombinant enzyme exhibits high thermal stability with half-lives of 6.3 h, 3.7 h and 52 min at 40, 45 and 50°C, respectively Candida orthopsilosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Candida orthopsilosis

pH Range

pH Minimum pH Maximum Comment Organism
6 8 more than 40% activity between pH 6.0 and 8.0 Candida orthopsilosis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Candida orthopsilosis