Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Clostridium sardiniense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sardiniense | G9FRD7 | - |
- |
Clostridium sardiniense DSM 599 | G9FRD7 | - |
- |
Purification (Comment) | Organism |
---|---|
GST-Sefinose column chromatography | Clostridium sardiniense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chenodeoxycholic acid + NADP+ | in combination with a second reaction step with EC 1.1.1.201, 7beta-hydroxysteroid dehydrogenase, to ursodeoxycholic acid | Clostridium sardiniense | 7-oxo-lithocholic acid + NADPH + H+ | - |
r | |
chenodeoxycholic acid + NADP+ | in combination with a second reaction step with EC 1.1.1.201, 7beta-hydroxysteroid dehydrogenase, to ursodeoxycholic acid | Clostridium sardiniense DSM 599 | 7-oxo-lithocholic acid + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Clostridium sardiniense |
Synonyms | Comment | Organism |
---|---|---|
7alpha-HSDH | - |
Clostridium sardiniense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Clostridium sardiniense |