Crystallization (Comment) | Organism |
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purified native and selenomethionine-labeled enzyme alone and in complex with NADP+ and sepiapterin, hanging drop vapour diffusion method, 18°C, 0.004 ml protein solution, with or without 10 mM NADP+ and sepiapterin, is mixed with 0.001 ml of a mixture of 0.004 ml or reservoir solution containing 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, and 34% PEG 400, and 0.001 ml additive solution 1 M guanidinium hydrochloride, X-ray diffraction structure determination and analysis at 2.1 A and 1.7 A resolution, respectively | Chlorobaculum tepidum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
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6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+ | Chlorobaculum tepidum | last step of the tetrahydrobiopterin biosynthesis | L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+ | - |
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Organism | UniProt | Comment | Textmining |
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Chlorobaculum tepidum | - |
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Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+ | active site structure, substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin, stereospecific hydride transfer, mechanism, structure-function relationship, overview | Chlorobaculum tepidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+ | last step of the tetrahydrobiopterin biosynthesis | Chlorobaculum tepidum | L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+ | - |
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6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+ | substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin, structure-function relationship | Chlorobaculum tepidum | L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+ | - |
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Subunits | Comment | Organism |
---|---|---|
More | structure-function relationship, amino acid sequence comparison of several species | Chlorobaculum tepidum |
Synonyms | Comment | Organism |
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SR | - |
Chlorobaculum tepidum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | determination and analysis of the binding structure of NADPH and NADP+ to the enzyme, overview | Chlorobaculum tepidum |