Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells | Vibrio cholerae serotype O1 |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 2.0-2.7 M ammonium sulfate and 0.1 M Tris (pH 9.0) | Vibrio cholerae serotype O1 |
Protein Variants | Comment | Organism |
---|---|---|
G141A | the mutant shows less than 5% of wild type activity | Vibrio cholerae serotype O1 |
G92A | the mutant shows less than 60% of wild type activity | Vibrio cholerae serotype O1 |
G92D | the mutant shows less than 5% of wild type activity | Vibrio cholerae serotype O1 |
Q152A | the mutant shows less than 10% of wild type activity | Vibrio cholerae serotype O1 |
Y155F | the mutant shows less than 3% of wild type activity | Vibrio cholerae serotype O1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
- |
Vibrio cholerae serotype O1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxoacyl-[acyl-carrier protein] + NADPH + H+ | Vibrio cholerae serotype O1 | - |
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex 200 pg gel filtration | Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxoacyl-[acyl-carrier protein] + NADPH + H+ | - |
Vibrio cholerae serotype O1 | (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | - |
Vibrio cholerae serotype O1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer or homotetramer | 2 or 4 * 25000, SDS-PAGE | Vibrio cholerae serotype O1 |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-(acyl carrier protein) reductase | - |
Vibrio cholerae serotype O1 |
FabG | - |
Vibrio cholerae serotype O1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Vibrio cholerae serotype O1 |