Activating Compound | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | 1 mM, 1.1fold activation | Devosia riboflavina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 6% inhibition | Devosia riboflavina | |
2,2'-bipyridyl | 1 mM, 11% inhibition | Devosia riboflavina | |
2,4-dinitrophenol | 1 mM, complete inhibition | Devosia riboflavina | |
2-mercaptoethanol | 1 mM, 19% inhibition | Devosia riboflavina | |
AgNO3 | 1 mM, 37% inhibition | Devosia riboflavina | |
BaCl2 | 1 mM, complete inhibition | Devosia riboflavina | |
dithiothreitol | 1 mM, 6% inhibition | Devosia riboflavina | |
EDTA | 1 mM, 31% inhibition | Devosia riboflavina | |
FeSO4 | 1 mM, complete inhibition | Devosia riboflavina | |
HgCl2 | 1 mM, complete inhibition | Devosia riboflavina | |
N-ethylmaleimide | 1 mM, 11% inhibition | Devosia riboflavina | |
PbCl2 | 1 mM, complete inhibition | Devosia riboflavina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
N-benzyl-3-pyrrolidinone | - |
Devosia riboflavina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM CuSO4, 1.7fold activation | Devosia riboflavina | |
Li+ | 1 mM LiCl, 1.1fold activation | Devosia riboflavina | |
Ni2+ | 1 mM NiSO4, 1.2fold activation | Devosia riboflavina | |
Zn2+ | 1 mM ZnSO4, 1.3fold activation | Devosia riboflavina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Devosia riboflavina |
58000 | - |
gel filtration | Devosia riboflavina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Devosia riboflavina | - |
KNK10702 | - |
Devosia riboflavina KNK10702 | - |
KNK10702 | - |
Purification (Comment) | Organism |
---|---|
- |
Devosia riboflavina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.12 | - |
- |
Devosia riboflavina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-butanol + NAD+ | 55% of activity with N-benzyl-3-pyrrolidinol | Devosia riboflavina | 2-butanone + NADH + H+ | - |
? | |
(R)-2-butanol + NAD+ | 55% of activity with N-benzyl-3-pyrrolidinol | Devosia riboflavina KNK10702 | 2-butanone + NADH + H+ | - |
? | |
1-octanol + NAD+ | 6% of activity with N-benzyl-3-pyrrolidinol | Devosia riboflavina | 1-octanal + NADH + H+ | - |
? | |
1-phenyl-2-butanone + NADH + H+ | 1.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | 1-phenylbutan-2-ol + NAD+ | - |
? | |
1-phenyl-2-butanone + NADH + H+ | 1.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina KNK10702 | 1-phenylbutan-2-ol + NAD+ | - |
? | |
2-acetylpyrrole + NADH + H+ | 70% of activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | 1-(1H-pyrrol-2-yl)ethanol + NAD+ | - |
? | |
2-butanone + NADH + H+ | 2.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | butan-2-ol + NAD+ | - |
? | |
2-hexanone + NADH + H+ | 50.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | hexan-2-ol + NAD+ | - |
? | |
2-nitrobenzaldehyde + NADH + H+ | 33% of activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | (2-nitrophenyl)methanol + NAD+ | - |
? | |
2-oxo-butyric acid + NADH + H+ | 15% of activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | 2-hydroxybutyric acid + NAD+ | - |
? | |
2-pentanol + NAD+ | 45% of activity with N-benzyl-3-pyrrolidinol | Devosia riboflavina | 2-pentanone + NADH + H+ | - |
? | |
2-pentanol + NAD+ | 45% of activity with N-benzyl-3-pyrrolidinol | Devosia riboflavina KNK10702 | 2-pentanone + NADH + H+ | - |
? | |
3-acetylpyridine + NADH + H+ | 7.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | 1-pyridin-3-ylethanol + NAD+ | - |
? | |
3-phenylpropionaldehyde + NADH + H+ | 6.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | 3-phenylpropanol + NAD+ | - |
? | |
4-acetylpyridine + NADH + H+ | 64.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | 1-pyridin-4-ylethanol + NAD+ | - |
? | |
acetone + NADH + H+ | 1.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | propan-2-ol + NAD+ | - |
? | |
acetophenone + NADH + H+ | 1.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | 1-phenylethanol + NAD+ | - |
? | |
benzaldehyde + NADH + H+ | 21% of activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | benzyl alcohol + NAD+ | - |
? | |
benzoin + NADH + H+ | 13% of activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | 1,2-diphenylethane-1,2-diol | - |
? | |
benzyloxycarbonyl-3-pyrrolidinone + NADH + H+ | production with 99.6% enantiomeric excess | Devosia riboflavina | (S)-benzyloxycarbonyl-3-pyrrolidinol + NAD+ | - |
? | |
chloroacetone + NADH + H+ | 41.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | 1-chloropropan-2-ol + NAD+ | - |
? | |
diacetyl + NADH + H+ | 16.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | ? | - |
? | |
diacetyl + NADH + H+ | 16.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina KNK10702 | ? | - |
? | |
dihydro-4,4-dimethyl-2,3-furandione + NADH + H+ | 13.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | ? | - |
? | |
ethyl 4-chloroacetoacetate + NADH + H+ | 6.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | ? | - |
? | |
ethyl acetoacetate + NADH + H+ | 42.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | ? | - |
? | |
glutaraldehyde + NADH + H+ | 70.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | ? | - |
? | |
hexanal + NADH + H+ | 22.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | n-hexanol + NAD+ | - |
? | |
levulinic acid + NADH + H+ | 11% of activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | ? | - |
? | |
m-chlorophenacyl chloride + NADH + H+ | 4.6fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | 2-chloro-1-(3-chlorophenyl)ethanol + NAD+ | - |
? | |
methyl glyoxal + NADH + H+ | 3.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | ? | - |
? | |
methyl pyruvate + NADH + H+ | 18.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | methyl 2-hydroxypropanoate + NAD+ | - |
? | |
additional information | when NADH is replaced with NADPH, the reaction rate is reduced by 0.6% | Devosia riboflavina | ? | - |
? | |
additional information | when NADH is replaced with NADPH, the reaction rate is reduced by 0.6% | Devosia riboflavina KNK10702 | ? | - |
? | |
N-benzyl-3-piperidone + NADH + H+ | 2.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | N-benzylpiperidin-3-ol + NAD+ | - |
? | |
N-benzyl-3-pyrrolidinone + NADH + H+ | (R)-stereoselectivity of the reduction carried out with the heat-treated cells | Devosia riboflavina | (R)-N-benzyl-3-pyrrolidinol + NAD+ | - |
r | |
N-benzyl-3-pyrrolidinone + NADH + H+ | - |
Devosia riboflavina | (S)-N-benzyl-3-pyrrolidinol + NAD+ | - |
r | |
N-benzyl-4-piperidone + NADH + H+ | 10.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | N-benzylpiperidin-4-ol + NAD+ | - |
? | |
n-butyraldehyde + NADH + H+ | 7.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | n-butanol + NAD+ | - |
? | |
N-tert-butoxycarbonyl-3-pyrrolidinone + NADH + H+ | production of (S)-N-tert-butoxycarbonyl-3-pyrrolidinol with 99.6% enantiomeric excess | Devosia riboflavina | (S)-N-tert-butoxycarbonyl-3-pyrrolidinol + NAD+ | - |
? | |
oxalacetic acid + NADH + H+ | 14% of activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | ? | - |
? | |
p-chlorobenzaldehyde + NADH + H+ | 1.2fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | p-chlorobenzyl alcohol + NAD+ | - |
? | |
p-nitrobenzyloxycarbonyl-3-pyrrolidinone + NADH + H+ | production with 90.7% enantiomeric excess | Devosia riboflavina | (S)-N-p-nitrobenzyloxycarbonyl-3-pyrrolidinol + NAD+ | - |
? | |
phenylethanol + NAD+ | 15% of activity with N-benzyl-3-pyrrolidinol | Devosia riboflavina | phenylacetaldehyde + NADH + H+ | - |
? | |
propiophenone + NADH + H+ | 27% of activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | 1-phenylpropan-1-ol + NAD+ | - |
? | |
pyridine-3-aldehyde + NADH + H+ | 82% of activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | pyridin-3-yl ethanol + NAD+ | - |
? | |
pyridine-4-aldehyde + NADH + H+ | 4.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone | Devosia riboflavina | pyridin-4-yl methanol + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE | Devosia riboflavina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Devosia riboflavina |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | 30°C: 36% of maximal activity, 65°C: 24% of maximal activity | Devosia riboflavina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
1 h, 90 mM potassium phosphate buffer, pH 6.5, stable | Devosia riboflavina |
45 | - |
1 h, 90 mM potassium phosphate buffer, pH 6.5, 70% loss of activity | Devosia riboflavina |
50 | - |
1 h, 90 mM potassium phosphate buffer, pH 6.5, complete loss of activity | Devosia riboflavina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | - |
- |
Devosia riboflavina |