Reference on EC 2.4.2.1 - purine-nucleoside phosphorylase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Agarwal, R.P.; Parks, R.E.
Purine nucleoside phosphorylase from human erythrocytes. IV. Crystallization and some properties
J. Biol. Chem.
244
644-647
1969
Bos taurus, Homo sapiens
Kamel, M.Y.; Fahmy, A.S.; Ghazy, A.H.; Mohamed, M.A.
Purification and characterization of purine nucleoside phosphorylase from developing embryos of Hyalomma dromedarii
Biochem. Cell Biol.
69
223-231
1991
Hyalomma dromedarii
Osman, A.M.; Del Corso, A.; Mohamed, A.S.; Ipata, P.L.; Mura, U.
Liver purine nucleoside phosphorylase in Camelus dromedarius: purification and properties
Comp. Biochem. Physiol. B
97
177-182
1990
Camelus dromedarius
Parks, R.E.; Agarwal, R.P.
Purine nucleoside phosphorylase
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
7
483-514
1972
Klebsiella aerogenes, Aeromonas hydrophila, Brevibacillus brevis, Bacillus cereus, Bacillus subtilis, Bacillus licheniformis, Bacterium cadaveris, Bos taurus, Canis lupus familiaris, Gallus gallus, Columba livia, Clavibacter michiganensis subsp. sepedonicus, Oryctolagus cuniculus, Escherichia coli, Equus caballus, Pectobacterium carotovorum, Felis catus, Homo sapiens, Lactobacillus leichmannii, Leucisus rusticus, Macaca mulatta, Micrococcus luteus, Papio hamadryas, Proteus vulgaris, Shewanella putrefaciens, Rattus norvegicus, salmon, Salmonella enterica subsp. enterica serovar Enteritidis, Sus scrofa
-
Cook, W.J.; Ealick, S.E.; Krenitsky, T.A.; Stoeckler, J.D.; Helliwell, J.R.; Bugg, C.E.
Crystallization and preliminary x-ray investigation of purine-nucleoside phosphorylase from Escherichia coli
J. Biol. Chem.
260
12968-12969
1985
Escherichia coli
Cook, W.J.; Ealick, S.E.; Bugg, C.E.; Stoeckler, J.D.; Parks, R.E.
Crystallization and preliminary X-ray investigation of human erythrocytic purine nucleoside phosphorylase
J. Biol. Chem.
256
4079-4080
1981
Homo sapiens
Murakami, K.; Tsushima, K.
Crystallization and some properties of purine nucleoside phosphorylase from chicken liver
Biochim. Biophys. Acta
384
390-398
1975
Gallus gallus
Lewis, A.S.; Lowy, B.A.
Human erythrocyte purine nucleoside phosphorylase: molecular weight and physical properties. A Theorell-Chance catalytic mechanism
J. Biol. Chem.
254
9927-9932
1979
Homo sapiens
Bzowska, A.; Kulikowska, E.; Shugar, D.
Formycins A and B and some analogues: selective inhibitors of bacterial (Escherichia coli) purine nucleoside phosphorylase
Biochim. Biophys. Acta
1120
239-247
1992
Escherichia coli
Robertson, B.C.; Hoffee, P.A.
Purification and properties of purine nucleoside phosphorylase from Salmonella typhimurium
J. Biol. Chem.
248
2040-2043
1973
Salmonella enterica subsp. enterica serovar Typhimurium
Jensen, K.J.
Two purine nucleoside phosphorylases in Bacillus subtilis. Purification and some properties of the adenosine-specific phosphorylase
Biochim. Biophys. Acta
525
346-356
1978
Bacillus subtilis
Schimandle, C.M.; Tanigoshi, L.; Mole, L.A.; Sherman, I.W.
Purine nucleoside phosphorylase of the malarial parasite, Plasmodium lophurae
J. Biol. Chem.
260
4455-4460
1985
Plasmodium lophurae
Umemura, S.; Nishino, T.; Murakami, K.; Tsushima, K.
Trimeric purine nucleoside phosphorylase from chicken liver having a proteolytic nick on each subunit and its kinetic properties
J. Biol. Chem.
257
13374-13378
1982
Gallus gallus
Osborne, W.R.A.
Human red cell purine nucleoside phosphorylase. Purification by biospecific affinity chromatography and physical properties
J. Biol. Chem.
255
7089-7092
1980
Homo sapiens
Surette, M.; Gill, T.; MacLean, S.
Purification and characterization of purine nucleoside phosphorylase from Proteus vulgaris
Appl. Environ. Microbiol.
56
1435-1439
1990
Proteus vulgaris
Murakami, K.; Tsushima, K.
Molecular properties and a nonidentical trimeric structure of purine nucleoside phosphorylase from chicken liver
Biochim. Biophys. Acta
435
205-210
1976
Gallus gallus
-
Hori, N.; Watanabe, M.; Yamazaki, Y.; Mikami, Y.
Purification and characterization of thermostable purine nucleoside phosphorylase of Bacillus stearothermophilus JTS 859
Agric. Biol. Chem.
53
2205-2210
1989
Geobacillus stearothermophilus, Geobacillus stearothermophilus JTS 859
-
Utagawa, T.; Morisawa, H.; Yamanaka, S.; Yamazaki, A.; Yoshinaga, F.; Hirose, Y.
Properties of nucleoside phosphorylase from Enterobacter aerogenes
Agric. Biol. Chem.
49
3239-3246
1985
Klebsiella aerogenes
-
Machida, Y.; Nakanishi, T.
Properties of purine nucleoside phosphorylase from Enterobacter cloacae
Agric. Biol. Chem.
45
1801-1807
1981
Enterobacter cloacae, Alcaligenes faecalis, Arthrobacter nucleogenes, Bacterium cadaveris, Corynebacterium vitaeruminis, Cellulomonas cellasea, Micrococcus luteus, Proteus mirabilis, Xanthomonas campestris, Cellulomonas cellasea KY3491, Alcaligenes faecalis KY3106, Xanthomonas campestris KY4208, Bacterium cadaveris KY3402, Proteus mirabilis KY4057, Micrococcus luteus KY 3760, Corynebacterium vitaeruminis KY3459, Arthrobacter nucleogenes KY3168, Enterobacter cloacae KY3074
-
Moyer, T.P.; Fischer, A.G.
Purification and characterization of a purine-nucleoside phosphorylase from bovine thyroid
Arch. Biochem. Biophys.
174
622-629
1976
Bos taurus
Shirae, H.; Yokozeki, K.
Purifications and properties of orotidine-phosphorolyzing enzyme and purine nucleoside phosphorylase from Erwinia carotovora AJ 2992
Agric. Biol. Chem.
55
1849-1857
1991
Pectobacterium carotovorum, Pectobacterium carotovorum AJ 2992
Hori, N.; Watanabe, M.; Yamazaki, Y.; Mikami, Y.
Purification and characterization of second thermostable purine nucleoside phosphorylase in Bacillus stearothermophilus JTS 859
Agric. Biol. Chem.
53
3219-3224
1989
Geobacillus stearothermophilus, Geobacillus stearothermophilus JTS 859
-
Shirae, H.; Yokozeki, K.
Purification and properties of purine nucleoside phosphorylase from Brevibacterium acetylicum ATCC 954
Agric. Biol. Chem.
55
493-499
1991
Exiguobacterium acetylicum
Hoffee, P.A.; May, R.; Robertson, B.C.
Purine nucleoside phosphorylase from Salmonella typhimurium and rat liver
Methods Enzymol.
51
517-524
1978
Rattus norvegicus, Salmonella enterica subsp. enterica serovar Typhimurium
Glantz, M.D.; Lewis, A.S.
Purine nucleoside phosphorylase from rabbit liver
Methods Enzymol.
51
524-530
1978
Oryctolagus cuniculus
Stoeckler, J.D.; Agarwal, R.P.; Agarwal, K.C.; Parks, R.E.
Purine nucleoside phosphorylase from human erythrocytes
Methods Enzymol.
51
530-538
1978
Homo sapiens
Milman, G.
Chinese hamster purine nucleoside phosphorylase
Methods Enzymol.
51
538-543
1978
Cricetulus griseus
Barsacchi, D.; Cappiello, M.; Tozzi, M.G.; Del Corso, A.; Peccatori, M.; Camici, M.; Ipata, P.L.; Mura, U.
Purine nucleoside phosphorylase from bovine lens: purification and properties
Biochim. Biophys. Acta
1160
163-170
1992
Bos taurus
May, R.A.; Hoffee, P.
Purine nucleoside phosphorylases purified from rat liver and Novikoff hepatoma cells
Arch. Biochem. Biophys.
193
398-406
1979
Rattus norvegicus
Milman, G.; Anton, D.L.; Weber, J.L.
Chinese hamster purine-nucleoside phosphorylase: purification, structural, and catalytic properties
Biochemistry
15
4967-4973
1976
Cricetulus griseus
Stoeckler, J.D.; Agarwal, R.P.; Agarwal, K.C.; Schmid, K.; Parks, R.E.
Purine nucleoside phosphorylase from human erythrocytes: physiocochemical properties of the crystalline enzyme
Biochemistry
17
278-283
1978
Homo sapiens
Lewis, A.S.; Glantz, M.D.
Bovine brain purine-nucleoside phosphorylase purification, characterization, and catalytic mechanism
Biochemistry
15
4451-4457
1976
Bos taurus
Bzowska, A.; Kulikowska, E.; Darzynkiewicz, E.; Shugar, D.
Purine nucleoside phosphorylase. Structure-activity relationships for substrate and inhibitor properties of N-1-, N-7-, and C-8-substituted analogues; differentiation of mammalian and bacterial enzymes with N-1-methylinosine and guanosine
J. Biol. Chem.
263
9212-9217
1988
Bos taurus, Escherichia coli
Zannis, V.; Doyle, D.; Martin, D.W.
Purification and characterization of human erythrocyte purine nucleoside phosphorylase and its subunits
J. Biol. Chem.
253
504-510
1978
Homo sapiens
Kulikowska, E.; Bzowska, A.; Wierzchowski, J.; Shugar, D.
Properties of two unusual, and fluorescent, substrates of purine-nucleoside phosphorylase: 7-methylguanosine and 7-methylinosine
Biochim. Biophys. Acta
874
355-363
1986
Bos taurus
Savage, B.; Spencer, N.
Partial purification and properties of purine nucleoside phosphorylase from rabbit erythrocytes
Biochem. J.
167
703-710
1977
Oryctolagus cuniculus
Lewis, A.S.; Glantz, M.D.
Monomeric purine nucleoside phosphorylase from rabbit liver. Purification and characterization
J. Biol. Chem.
251
407-413
1976
Oryctolagus cuniculus
Jensen, K.F.; Nygaard, P.
Purine nucleoside phosphorylase from Escherichia coli and Salmonella typhimurium. Purification and some properties
Eur. J. Biochem.
51
253-265
1975
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium
Bzowska, A.; Kulikowska, E.; Shugar, D.
Properties of purine nucleoside phosphorylase (PNP) of mammalian and bacterial origin
Z. Naturforsch. C
45c
59-70
1990
Bos taurus, Escherichia coli, Homo sapiens
-
Lewis, A.S.
Rabbit brain purine nucleoside phosphorylase. Physical and chemical properties. Inhibition studies with aminopterin, folic acid and structurally related compounds
Arch. Biochem. Biophys.
190
662-670
1978
Oryctolagus cuniculus
Divekar, A.Y.
Adenosine phosphyorylase activity as distinct from inosine-guanosine phosphorylase activity in Sarcoma 180 cells and rat liver
Biochim. Biophys. Acta
422
15-28
1976
Rattus norvegicus
Daddona, P.E.; Wiesmann, W.P.; Milhouse, W.; Chern, J.W.; Townsend, L.B.; Hershfield, M.S.; Webster, H.K.
Expression of human malaria parasite purine nucleoside phosphorylase in host enzyme-deficient erythrocyte culture. Enzyme characterization and identification of novel inhibitors
J. Biol. Chem.
261
11667-11673
1986
Homo sapiens, Plasmodium falciparum
Koszalka, G.W.; Vanhooke, J.; Short, S.A.; Hall, W.W.
Purification and properties of inosine-guanosine phosphorylase from Escherichia coli K-12
J. Bacteriol.
170
3493-3498
1988
Escherichia coli
Mao, C.; Cook, W.J.; Zhou, M.; Federov, A.A.; Almo, S.C.; Ealick, S.E.
Calf spleen purine nucleoside phosphorylase complexed with substrates and substrate analogues
Biochemistry
36
7135-7146
1998
Bos taurus
-
Bzowska, A.; Kulikowska, E.; Poopeiko, N.E.; Shugar, D.
Kinetics of phosphorolysis of 3-(beta-D-ribofuranosyl)adenine and 3-(beta-D-ribofuranosyl)hypoxanthine, non-conventional substrates of purine-nucleoside phosphorylase
Eur. J. Biochem.
239
229-234
1996
Bos taurus, Escherichia coli
Wielgus-Kutrowska, B.; Bzowska, A.; Tebbe, J.; Koeller, G.; Shugar, D.
Purine nucleoside phosphorylase from Cellulomonas sp.: physicochemical properties and binding of substrates determined by ligand-dependent enhancement of enzyme intrinsic fluorescence, and by protective effects of ligands on thermal inactivation of the enzyme
Biochim. Biophys. Acta
1597
320-334
2002
Cellulomonas sp.
Kline, P.C.; Schramm, V.L.
Purine nucleoside phosphorylase. Catalytic mechanism and transition-state analysis of the arsenolysis reaction
Biochemistry
32
13212-13219
1993
Bos taurus
Hamamoto, T.; Noguchi, T.; Midorikawa, Y.
Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2
Biosci. Biotechnol. Biochem.
60
1179-1180
1996
Geobacillus stearothermophilus, Geobacillus stearothermophilus TH 6-2
Munagala, N.; Wang, C.C.
The purine nucleoside phosphorylase from Trichomonas vaginalis is a homologue of the bacterial enzyme
Biochemistry
41
10382-10389
2002
Trichomonas vaginalis
Mao, C.; Cook, W.J.; Zhou, M.; Koszalka, G.W.; Krenitsky, T.A.; Ealick, S.E.
The crystal structure of Escherichia coli purine nucleoside phosphorylase: a comparison with the human enzyme reveals a conserved topology
Structure
5
1373-1383
1997
Escherichia coli
Koeller, G.; Luic, M.; Shugar, D.; Saenger, W.; Bzowska, A.
Crystal structure of calf spleen purine nucleoside phosphorylase in a complex with hypoxanthine at 2.15 A resolution
J. Mol. Biol.
265
202-216
1997
Bos taurus (P55859)
Koellner, G.; Bzowska, A.; Wielgus-Kutrowska, B.; Luic, M.; Steiner, T.; Saenger, W.; Stepinski, J.
Open and closed conformation of the E. coli purine nucleoside phosphorylase active center and implications for the catalytic mechanism
J. Mol. Biol.
315
351-371
2002
Escherichia coli (P0ABP8), Escherichia coli
Choi, H.S.
Purification and partial characterization of purine nucleoside phosphorylase from Serratia marcescens
Biosci. Biotechnol. Biochem.
62
667-671
1998
Serratia marcescens
Lee, J.; Filosa, S.; Bonvin, J.; Guyon, S.; Aponte, R.A.; Turnbull, J.L.
Expression, purification, and characterization of recombinant purine nucleoside phosphorylase from Escherichia coli
Protein Expr. Purif.
22
180-188
2001
Cellulomonas sp., Escherichia coli
Erion, M.D.; Takabayashi, K.; Smith, H.B.; Kessi, J.; Wagner, S.; Hoenger, S.; Shames, S.L.; Ealick, S.E.
Purine nucleoside phosphorylase. 1. Structure-function Studies
Biochemistry
36
11725-11734
1997
Homo sapiens
Maynes, J.T.; Yam, W.S.; Jenuth, J.P.; Yuan, R.G.; Litster, S.A.; Phipps, B.M.; Snyder, F.F.
Design of an adenosine phosphorylase by active-site modification of murine purine nucleoside phosphorylase: enzyme kinetics and molecular dynamics simulation of Asn-243 and Lys-244 substitutions of purine nucleoside phosphorylase
Biochem. J.
344
585-592
1999
Mus musculus
-
Stoychev, G.; Kierdaszuk, B.; Shugar, D.
Xanthosine and xanthine. Substrate properties with purine nucleoside phosphorylases, and relevance to other enzyme systems
Eur. J. Biochem.
269
4048-4057
2002
Homo sapiens, Bos taurus (P55859)
Wielgus-Kutrowska, B.; Tebbe, J.; Schroder, W.; Luic, M.; Shugar, D.; Saenger, W.; Koellner, G.; Bzowska, A.
Cellulomonas sp. purine nucleoside phosphorylase (PNP): comparison with human and E. coli enzymes
Adv. Exp. Med. Biol.
431
259-264
1998
Cellulomonas sp.
Montgomery, J.A.
Purine nucleoside phosphorylase: a target for drug design
Med. Res. Rev.
13
209-228
1993
Bos taurus, Homo sapiens
Cheng, J.; Farutin, V.; Wu, Z.; Jacob-Mosier, G.; Riley, B.; Hakimi, R.; Cordes, E.H.
Purine nucleoside phosphorylase-catalyzed, phosphate-independent hydrolysis of 2-amino-6-mercapto-7-methylpurine ribonucleoside
Bioorg. Chem.
27
307-325
1999
Bos taurus
-
Bzowska, A.; Kulikowska, E.; Shugar, D.
Purine nucleoside phosphorylases: properties, functions, and clinical aspects
Pharmacol. Ther.
88
349-425
2000
Acholeplasma laidlawii, Klebsiella aerogenes, Geobacillus stearothermophilus, Bacillus cereus, Cellulomonas sp., Pectobacterium carotovorum, Fasciola hepatica, Klebsiella sp., Micrococcus luteus, Mus musculus, Plasmodium falciparum, Plasmodium lophurae, Proteus vulgaris, Rattus norvegicus, Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens, Saccharolobus solfataricus, Trypanosoma brucei, Trypanosoma cruzi, Homo sapiens (P00491), Homo sapiens, Escherichia coli (P0ABP8), Bacillus subtilis (P46354), Bos taurus (P55859), Saccharomyces cerevisiae (Q05788)
Luic, M.; Koellner, G.; Yokomatsu, T.; Shibuya, S.; Bzowska, A.
Calf spleen purine-nucleoside phosphorylase: crystal structure of the binary complex with a potent multisubstrate analogue inhibitor
Acta Crystallogr. Sect. D
60
1417-1424
2004
Bos taurus
Schnick, C.; Robien, M.A.; Brzozowski, A.M.; Dodson, E.J.; Murshudov, G.N.; Anderson, L.; Luft, J.R.; Mehlin, C.; Hol, W.G.; Brannigan, J.A.; Wilkinson, A.J.
Structures of Plasmodium falciparum purine nucleoside phosphorylase complexed with sulfate and its natural substrate inosine
Acta Crystallogr. Sect. D
61
1245-1254
2005
Plasmodium falciparum
Grenha, R.; Levdikov, V.M.; Fogg, M.J.; Blagova, E.V.; Brannigan, J.A.; Wilkinson, A.J.; Wilson, K.S.
Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis
Acta Crystallogr. Sect. F
61
459-462
2005
Bacillus anthracis (Q81T09), Bacillus anthracis
Silva, R.G.; Pereira, J.H.; Canduri, F.; de Azevedo, W.F., Jr.; Basso, L.A.; Santos, D.S.
Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine
Arch. Biochem. Biophys.
442
49-58
2005
Homo sapiens
Canduri, F.; Fadel, V.; Basso, L.A.; Palma, M.S.; Santos, D.S.; de Azevedo, W.F., Jr.
New catalytic mechanism for human purine nucleoside phosphorylase
Biochem. Biophys. Res. Commun.
327
646-649
2005
Homo sapiens (P00491), Homo sapiens
Bennett, E.M.; Li, C.; Allan, P.W.; Parker, W.B.; Ealick, S.E.
Structural basis for substrate specificity of Escherichia coli purine nucleoside phosphorylase
J. Biol. Chem.
278
47110-47118
2003
Escherichia coli (P0ABP8), Escherichia coli
Shi, W.; Ting, L.M.; Kicska, G.A.; Lewandowicz, A.; Tyler, P.C.; Evans, G.B.; Furneaux, R.H.; Kim, K.; Almo, S.C.; Schramm, V.L.
Plasmodium falciparum purine nucleoside phosphorylase: crystal structures, immucillin inhibitors, and dual catalytic function
J. Biol. Chem.
279
18103-18106
2004
Plasmodium falciparum, Homo sapiens (P00491), Homo sapiens
Zang, Y.; Wang, W.H.; Wu, S.W.; Ealick, S.E.; Wang, C.C.
Identification of a subversive substrate of Trichomonas vaginalis purine nucleoside phosphorylase and the crystal structure of the enzyme-substrate complex
J. Biol. Chem.
280
22318-22325
2005
Trichomonas vaginalis
Tahirov, T.H.; Inagaki, E.; Ohshima, N.; Kitao, T.; Kuroishi, C.; Ukita, Y.; Takio, K.; Kobayashi, M.; Kuramitsu, S.; Yokoyama, S.; Miyano, M.
Crystal structure of purine nucleoside phosphorylase from Thermus thermophilus
J. Mol. Biol.
337
1149-1160
2004
Thermus thermophilus
Dandanell, G.; Szczepanowski, R.H.; Kierdaszuk, B.; Shugar, D.; Bochtler, M.
Escherichia coli purine nucleoside phosphorylase II, the product of the xapA gene
J. Mol. Biol.
348
113-125
2005
Escherichia coli (P45563), Escherichia coli
Pereira, H.D.; Franco, G.R.; Cleasby, A.; Garratt, R.C.
Structures for the potential drug target purine nucleoside phosphorylase from Schistosoma mansoni causal agent of Schistosomiasis
J. Mol. Biol.
353
584-599
2005
Schistosoma mansoni
Silva, R.G.; Santos, D.S.; Basso, L.A.; Oses, J.P.; Wofchuk, S.; Portela, L.V.; Souza, D.O.
Purine nucleoside phosphorylase activity in rat cerebrospinal fluid
Neurochem. Res.
29
1831-1835
2004
Rattus norvegicus
Iwanow, M.; Magnowska, L.; Yokomatsu, T.; Shibuya, S.; Bzowska, A.
Interactions of potent multisubstrate analogue inhibitors with purine nucleoside phosphorylase from calf spleen--kinetic and spectrofluorimetric studies
Nucleosides Nucleotides Nucleic Acids
22
1567-1570
2003
Bos taurus
Silva, R.G.; Carvalho, L.P.; Oliveira, J.S.; Pinto, C.A.; Mendes, M.A.; Palma, M.S.; Basso, L.A.; Santos, D.S.
Cloning, overexpression, and purification of functional human purine nucleoside phosphorylase
Protein Expr. Purif.
27
158-164
2003
Homo sapiens
Wierzchowski, J.; Bzowska, A.; Stepniak, K.; Shugar, D.
Interactions of calf spleen purine nucleoside phosphorylase with 8-azaguanine, and a bisubstrate analogue inhibitor: implications for the reaction mechanism
Z. Naturforsch. C
59
713-725
2004
Bos taurus
Toms, A.V.; Wang, W.; Li, Y.; Ganem, B.; Ealick, S.E.
Novel multisubstrate inhibitors of mammalian purine nucleoside phosphorylase
Acta Crystallogr. Sect. D
D61
1449-1458
2005
Bos taurus (P55859), Bos taurus
Canduri, F.; Silva, R.G.; Marangoni dos Santos, D.; Palma, M.S.; Basso, L.A.; Santos, D.S.; Filgueira de Azevedo, W.J.
Structure of human PNP complexed with ligands
Acta Crystallogr. Sect. D
D61
856-862
2005
Homo sapiens (P00491), Homo sapiens
Murkin, A.S.; Birck, M.R.; Rinaldo-Matthis, A.; Shi, W.; Taylor, E.A.; Almo, S.C.; Schramm, V.L.
Neighboring group participation in the transition state of human purine nucleoside phosphorylase
Biochemistry
46
5038-5049
2007
Homo sapiens (P00491), Homo sapiens
Rinaldo-Matthis, A.; Wing, C.; Ghanem, M.; Deng, H.; Wu, P.; Gupta, A.; Tyler, P.C.; Evans, G.B.; Furneaux, R.H.; Almo, S.C.; Wang, C.C.; Schramm, V.L.
Inhibition and structure of Trichomonas vaginalis purine nucleoside phosphorylase with picomolar transition state analogues
Biochemistry
46
659-668
2007
Trichomonas vaginalis, Homo sapiens (P00491), Homo sapiens, Bos taurus (P55859), Plasmodium falciparum (Q8T9Z7)
Wielgus-Kutrowska, B.; Bzowska, A.
Probing the mechanism of purine nucleoside phosphorylase by steady-state kinetic studies and ligand binding characterization determined by fluorimetric titrations
Biochim. Biophys. Acta
1764
887-902
2006
Cellulomonas sp. (P81989), Cellulomonas sp.
Castilho, M.S.; Postigo, M.P.; de Paula, C.B.; Montanari, C.A.; Oliva, G.; Andricopulo, A.D.
Two- and three-dimensional quantitative structure-activity relationships for a series of purine nucleoside phosphorylase inhibitors
Bioorg. Med. Chem.
14
516-527
2006
Bos taurus (P55859)
Kikuchi, E.; Menendez, S.; Ozu, C.; Ohori, M.; Cordon-Cardo, C.; Logg, C.R.; Kasahara, N.; Bochner, B.H.
Delivery of replication-competent retrovirus expressing Escherichia coli purine nucleoside phosphorylase increases the metabolism of the prodrug, fludarabine phosphate and suppresses the growth of bladder tumor xenografts
Cancer Gene Ther.
14
279-286
2007
Escherichia coli
Silva, R.G.; Nunes, J.E.; Canduri, F.; Borges, J.C.; Gava, L.M.; Moreno, F.B.; Basso, L.A.; Santos, D.S.
Purine nucleoside phosphorylase: a potential target for the development of drugs to treat T-cell- and apicomplexan parasite-mediated diseases
Curr. Drug Targets
8
413-422
2007
Bos taurus (P55859), Homo sapiens (P00491), Homo sapiens, Plasmodium falciparum (Q8T9Z7), Plasmodium lophurae, Toxoplasma gondii
Cacciapuoti, G.; Gorassini, S.; Mazzeo, M.F.; Siciliano, R.A.; Carbone, V.; Zappia, V.; Porcelli, M.
Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus
FEBS J.
274
2482-2495
2007
Pyrococcus furiosus, Pyrococcus furiosus (Q8U2I1)
Chaudhary, K.; Ting, L.M.; Kim, K.; Roos, D.S.
Toxoplasma gondii purine nucleoside phosphorylase biochemical characterization, inhibitor profiles, and comparison with the Plasmodium falciparum ortholog
J. Biol. Chem.
281
25652-25658
2006
Toxoplasma gondii (Q2HXR2), Toxoplasma gondii, Plasmodium falciparum (Q8T9Z7), Plasmodium falciparum
Toro, A.; Grunebaum, E.
TAT-mediated intracellular delivery of purine nucleoside phosphorylase corrects its deficiency in mice
J. Clin. Invest.
116
2717-2726
2006
Homo sapiens (P00491), Homo sapiens
Semeraro, T.; Lossani, A.; Botta, M.; Ghiron, C.; Alvarez, R.; Manetti, F.; Mugnaini, C.; Valensin, S.; Focher, F.; Corelli, F.
Simplified analogues of immucillin-G retain potent human purine nucleoside phosphorylase inhibitory activity
J. Med. Chem.
49
6037-6045
2006
Homo sapiens (P00491), Homo sapiens
Nunez, S.; Wing, C.; Antoniou, D.; Schramm, V.L.; Schwartz, S.D.
Insight into catalytically relevant correlated motions in human purine nucleoside phosphorylase
J. Phys. Chem. A
110
463-472
2006
Homo sapiens (P00491), Homo sapiens
Gherardi, A.; Peyrol, S.; Sarciron, M.E.
Toxoplasma gondii: localization of purine nucleoside phosphorylase activity in vitro and in vivo by electron microscopy
Med. Mol. Morphol.
38
251-255
2005
Toxoplasma gondii (Q2HXR2), Toxoplasma gondii, Toxoplasma gondii ME49 (Q2HXR2), Toxoplasma gondii ME49
Parker, W.B.; Allan, P.W.; Ealick, S.E.; Sorscher, E.J.; Hassan, A.E.; Silamkoti, A.V.; Fowler, A.T.; Waud, W.R.; Secrist, J.A.
Design and evaluation of 5-modified nucleoside analogs as prodrugs for an E. coli purine nucleoside phosphorylase mutant
Nucleosides Nucleotides Nucleic Acids
24
387-392
2005
Escherichia coli
Wierzchowski, J.; Stepniak, K.; Bzowska, A.; Shugar, D.
Spectroscopic and kinetic studies of interactions of calf spleen purine nucleoside phosphorylase with 8-azaguanine, and its 9-(2-phosphonylmethoxyethyl) derivative
Nucleosides Nucleotides Nucleic Acids
24
459-464
2005
Bos taurus
Wielgus-Kutrowska, B.; Bzowska, A.
Kinetic properties of Cellulomonas sp. purine nucleoside phosphorylase with typical and non-typical substrates: implications for the reaction mechanism
Nucleosides Nucleotides Nucleic Acids
24
471-476
2005
Cellulomonas sp.
Modrak-Wojcik, A.; Stepniak, K.; Akoev, V.; Zolkiewski, M.; Bzowska, A.
Molecular architecture of E. coli purine nucleoside phosphorylase studied by analytical ultracentrifugation and CD spectroscopy
Protein Sci.
15
1794-1800
2006
Escherichia coli
Taylor, E.A.; Rinaldo-Matthis, A.; Li, L.; Ghanem, M.; Hazleton, K.Z.; Cassera, M.B.; Almo, S.C.; Schramm, V.L.
Anopheles gambiae purine nucleoside phosphorylase: catalysis, structure, and inhibition
Biochemistry
46
12405-12415
2007
Plasmodium falciparum, Anopheles gambiae (A4Q998), Anopheles gambiae, Homo sapiens (P00491), Homo sapiens
Wielgus-Kutrowska, B.; Antosiewicz, J.M.; Dlgosz, M.; Holy, A.; Bzowska, A.
Towards the mechanism of trimeric purine nucleoside phosphorylases: Stopped-flow studies of binding of multisubstrate analogue inhibitor - 2-amino-9-[2-(phosphonomethoxy)ethyl]-6-sulfanylpurine
Biophys. Chem.
125
260-268
2007
Cellulomonas sp.
Saen-Oon, S.; Ghanem, M.; Schramm, V.L.; Schwartz, S.D.
Remote mutations and active site dynamics correlate with catalytic properties of purine nucleoside phosphorylase
Biophys. J.
94
4078-4088
2008
Homo sapiens (P00491), Homo sapiens
Li, X.; Jiang, X.; Li, H.; Ren, D.
Purine nucleoside phosphorylase from Pseudoalteromonas sp. Bsi590: molecular cloning, gene expression and characterization of the recombinant protein
Extremophiles
12
325-333
2008
Escherichia coli, Pseudoalteromonas sp. (A3F8V1), Pseudoalteromonas sp., Pseudoalteromonas sp. Bsi590 (A3F8V1)
Pereira, H.M.; Berdini, V.; Cleasby, A.; Garratt, R.C.
Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue
FEBS Lett.
581
5082-5086
2007
Bos taurus (P55859), Bos taurus
Rinaldo-Matthis, A.; Murkin, A.S.; Ramagopal, U.A.; Clinch, K.; Mee, S.P.; Evans, G.B.; Tyler, P.C.; Furneaux, R.H.; Almo, S.C.; Schramm, V.L.
L-Enantiomers of transition state analogue inhibitors bound to human purine nucleoside phosphorylase
J. Am. Chem. Soc.
130
842-844
2008
Homo sapiens (P00491), Homo sapiens
Mason, J.M.; Murkin, A.S.; Li, L.; Schramm, V.L.; Gainsford, G.J.; Skelton, B.W.
A beta-fluoroamine inhibitor of purine nucleoside phosphorylase
J. Med. Chem.
51
5880-5884
2008
Homo sapiens
Wierzchowski, J.; Iwanska, B.; Bzowska, A.; Shugar, D.
Interactions of calf spleen purine nucleoside phosphorylase with formycin B and its aglycone - spectroscopic and kinetic studies
Nucleosides Nucleotides Nucleic Acids
26
849-854
2007
Bos taurus
Stepniak, K.; Girstun, A.; Wielgus-Kutrowska, B.; Staro?, K.; Bzowska, A.
Cloning, expression, purification, and some properties of calf purine nucleoside phosphorylase
Nucleosides Nucleotides Nucleic Acids
26
855-859
2007
Bos taurus
Antosiewicz, J.; Wielgus-Kutrowska, B.; Dlugosz, M.; Holy, A.; Bzowska, A.
Kinetics of binding of multisubstrate analogue inhibitor (2-amino-9-[2-(phosphonomethoxy)ethyl]-6-sulfanylpurine) with trimeric purine nucleoside phosphorylase
Nucleosides Nucleotides Nucleic Acids
26
969-974
2007
Cellulomonas sp.
Glavas-Obrovac, L.; Suver, M.; Hikishima, S.; Yokomatsu, T.; Bzowska, A.
Inhibitory properties of nucleotides with difluoromethylenephosphonic acid as a phosphate mimic versus calf spleen purine nucleoside phosphorylase and effect of these analogues on the viability of human blood lymphocytes
Nucleosides Nucleotides Nucleic Acids
26
989-993
2007
Bos taurus
Breer, K.; Girstun, A.; Wielgus-Kutrowska, B.; Staro?, K.; Bzowska, A.
Overexpression, purification and characterization of functional calf purine nucleoside phosphorylase (PNP)
Protein Expr. Purif.
61
122-130
2008
Bos taurus (P55859), Bos taurus
Shi, Z.; Yang, W.Z.; Lin-Chao, S.; Chak, K.F.; Yuan, H.S.
Crystal structure of Escherichia coli PNPase: Central channel residues are involved in processive RNA degradation
RNA
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2361-2371
2008
Escherichia coli
Furman, R.R.; Hoelzer, D.
Purine nucleoside phosphorylase inhibition as a novel therapeutic approach for B-cell lymphoid malignancies
Semin. Oncol.
34
S29-S34
2007
Homo sapiens
Kang, Y.N.; Zhang, Y.; Allan, P.W.; Parker, W.B.; Ting, J.W.; Chang, C.Y.; Ealick, S.E.
Structure of grouper iridovirus purine nucleoside phosphorylase
Acta Crystallogr. Sect. D
66
155-162
2010
grouper iridovirus
Pereira, H.M.; Rezende, M.M.; Castilho, M.S.; Oliva, G.; Garratt, R.C.
Adenosine binding to low-molecular-weight purine nucleoside phosphorylase: the structural basis for recognition based on its complex with the enzyme from Schistosoma mansoni
Acta Crystallogr. Sect. D
66
73-79
2010
Schistosoma mansoni (Q9BMI9), Schistosoma mansoni
Hou, Q.M.; Liu, X.; Brostromer, E.; Li, L.F.; Su, X.D.
Preliminary crystallographic studies of purine nucleoside phosphorylase from the cariogenic pathogen Streptococcus mutans
Acta Crystallogr. Sect. F
65
1289-1291
2009
Streptococcus mutans
Pereira, H.M.; Berdini, V.; Ferri, M.R.; Cleasby, A.; Garratt, R.C.
Crystal structure of Schistosoma purine nucleoside phosphorylase complexed with a novel monocyclic inhibitor
Acta Trop.
114
97-102
2010
Schistosoma mansoni (Q9BMI9)
Timmers, L.F.; Caceres, R.A.; Vivan, A.L.; Gava, L.M.; Dias, R.; Ducati, R.G.; Basso, L.A.; Santos, D.S.; de Azevedo, W.F.
Structural studies of human purine nucleoside phosphorylase: towards a new specific empirical scoring function
Arch. Biochem. Biophys.
479
28-38
2008
Homo sapiens (P00491), Homo sapiens
Todorova, N.A.; Schwarz, F.P.
Effect of the phosphate substrate on drug-inhibitor binding to human purine nucleoside phosphorylase
Arch. Biochem. Biophys.
480
122-131
2008
Homo sapiens
Ducati, R.G.; Santos, D.S.; Basso, L.A.
Substrate specificity and kinetic mechanism of purine nucleoside phosphorylase from Mycobacterium tuberculosis
Arch. Biochem. Biophys.
486
155-164
2009
Mycobacterium tuberculosis
Breer, K.; Wielgus-Kutrowska, B.; Girstun, A.; Staro?, K.; Hashimoto, M.; Hikishima, S.; Yokomatsu, T.; Bzowska, A.
Overexpressed proteins may act as mops removing their ligands from the host cells: a case study of calf PNP
Biochem. Biophys. Res. Commun.
391
1203-1209
2010
Bos taurus
Ghanem, M.; Li, L.; Wing, C.; Schramm, V.L.
Altered thermodynamics from remote mutations altering human toward bovine purine nucleoside phosphorylase
Biochemistry
47
2559-2564
2008
Bos taurus, Homo sapiens
Luo, M.; Li, L.; Schramm, V.L.
Remote mutations alter transition-state structure of human purine nucleoside phosphorylase
Biochemistry
47
2565-2576
2008
Homo sapiens
Li, L.; Luo, M.; Ghanem, M.; Taylor, E.A.; Schramm, V.L.
Second-sphere amino acids contribute to transition-state structure in bovine purine nucleoside phosphorylase
Biochemistry
47
2577-2583
2008
Homo sapiens (P00491), Homo sapiens, Bos taurus (P55859), Bos taurus
Edwards, A.A.; Mason, J.M.; Clinch, K.; Tyler, P.C.; Evans, G.B.; Schramm, V.L.
Altered enthalpy-entropy compensation in picomolar transition state analogues of human purine nucleoside phosphorylase
Biochemistry
48
5226-5238
2009
Homo sapiens (P00491), Homo sapiens
Edwards, A.A.; Tipton, J.D.; Brenowitz, M.D.; Emmett, M.R.; Marshall, A.G.; Evans, G.B.; Tyler, P.C.; Schramm, V.L.
Conformational States of human purine nucleoside phosphorylase at rest, at work, and with transition state analogues
Biochemistry
49
2058-2067
2010
Homo sapiens (P00491), Homo sapiens
Kamath, V.P.; Xue, J.; Juarez-Brambila, J.J.; Morris, C.B.; Ganorkar, R.; Morris, P.E.
Synthesis of analogs of forodesine HCl, a human purine nucleoside phosphorylase inhibitor-Part I
Bioorg. Med. Chem. Lett.
19
2624-2626
2009
Homo sapiens
Castilho, M.S.; Postigo, M.P.; Pereira, H.M.; Oliva, G.; Andricopulo, A.D.
Structural basis for selective inhibition of purine nucleoside phosphorylase from Schistosoma mansoni: kinetic and structural studies
Bioorg. Med. Chem.
18
1421-1427
2010
Schistosoma mansoni, Homo sapiens (P00491), Homo sapiens
Timmers, L.F.; Caceres, R.A.; Dias, R.; Basso, L.A.; Santos, D.S.; de Azevedo, W.F.
Molecular modeling, dynamics and docking studies of purine nucleoside phosphorylase from Streptococcus pyogenes
Biophys. Chem.
142
7-16
2009
Streptococcus pyogenes
Cao, Y.; Wang, J.; Xu, Y.; Li, G.
Sensing purine nucleoside phosphorylase activity by using silver nanoparticles
Biosens. Bioelectron.
25
1032-1036
2010
Homo sapiens
Cai, X.; Zhou, J.; Chang, Y.; Sun, X.; Li, P.; Lin, J.
Targeting gene therapy for hepatocarcinoma cells with the E. coli purine nucleoside phosphorylase suicide gene system directed by a chimeric alpha-fetoprotein promoter
Cancer Lett.
264
71-82
2008
Escherichia coli
Glavas-Obrovac, L.; Suver, M.; Hikishima, S.; Hashimoto, M.; Yokomatsu, T.; Magnowska, L.; Bzowska, A.
Antiproliferative activity of purine nucleoside phosphorylase multisubstrate analogue inhibitors containing difluoromethylene phosphonic acid against leukaemia and lymphoma cells
Chem. Biol. Drug Des.
75
392-399
2010
Bos taurus, Homo sapiens
Deng, H.; Cross, S.M.; McGlinchey, R.P.; Hamilton, J.T.; OHagan, D.
In vitro reconstituted biotransformation of 4-fluorothreonine from fluoride ion: application of the fluorinase
Chem. Biol.
15
1268-1276
2008
Streptomyces cattleya
Kamath, V.P.; Juarez-Brambila, J.J.; Morris, P.E.
Synthesis of labeled BCX-4208, a potent inhibitor of purine nucleoside phosphorylase
Drug Test. Anal.
1
125-127
2009
Homo sapiens
Aytekin, C.; Dogu, F.; Tanir, G.; Guloglu, D.; Santisteban, I.; Hershfield, M.S.; Ikinciogullari, A.
Purine nucleoside phosphorylase deficiency with fatal course in two sisters
Eur. J. Pediatr.
169
311-314
2010
Homo sapiens
Visser, D.F.; Hennessy, F.; Rashamuse, K.; Louw, M.E.; Brady, D.
Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36
Extremophiles
14
185-192
2010
Halalkalibacterium halodurans (C7FLN0), Halalkalibacterium halodurans Alk36 (C7FLN0)
Cacciapuoti, G.; Peluso, I.; Fuccio, F.; Porcelli, M.
Purine nucleoside phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding
FEBS J.
276
5799-5805
2009
Pyrococcus furiosus
Bourdelas, A.; Li, H.Y.; Carron, C.; Shi, D.L.
Dynamic expression pattern of distinct genes in the presomitic and somitic mesoderm during Xenopus development
Int. J. Dev. Biol.
53
1075-1079
2009
Xenopus laevis
Madrid, D.C.; Ting, L.M.; Waller, K.L.; Schramm, V.L.; Kim, K.
Plasmodium falciparum purine nucleoside phosphorylase is critical for viability of malaria parasites
J. Biol. Chem.
283
35899-35907
2008
Plasmodium falciparum (Q8T9Z7), Plasmodium falciparum
Afshar, S.; Olafsen, T.; Wu, A.; Morrison, S.
Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPT
J. Exp. Clin. Cancer Res.
28
147
2009
Homo sapiens
Liao, P.; Toro, A.; Min, W.; Lee, S.; Roifman, C.M.; Grunebaum, E.
Lentivirus gene therapy for purine nucleoside phosphorylase deficiency
J. Gene Med.
10
1282-1293
2008
Homo sapiens
Clinch, K.; Evans, G.B.; Froehlich, R.F.; Furneaux, R.H.; Kelly, P.M.; Legentil, L.; Murkin, A.S.; Li, L.; Schramm, V.L.; Tyler, P.C.; Woolhouse, A.D.
Third-generation immucillins: syntheses and bioactivities of acyclic immucillin inhibitors of human purine nucleoside phosphorylase
J. Med. Chem.
52
1126-1143
2009
Homo sapiens, Mus musculus
Pauli, I.; Timmers, L.F.; Caceres, R.A.; Basso, L.A.; Santos, D.S.; de Azevedo, W.F.
Molecular modeling and dynamics studies of purine nucleoside phosphorylase from Bacteroides fragilis
J. Mol. Model.
15
913-922
2009
Bacteroides fragilis
Zanchi, F.B.; Caceres, R.A.; Stabeli, R.G.; de Azevedo, W.F.
Molecular dynamics studies of a hexameric purine nucleoside phosphorylase
J. Mol. Model.
16
543-550
2010
Plasmodium falciparum
Caceres, R.A.; Timmers, L.F.; Pauli, I.; Gava, L.M.; Ducati, R.G.; Basso, L.A.; Santos, D.S.; de Azevedo, W.F.
Crystal structure and molecular dynamics studies of human purine nucleoside phosphorylase complexed with 7-deazaguanine
J. Struct. Biol.
169
379-388
2010
Homo sapiens (P00491), Homo sapiens
Ting, L.M.; Gissot, M.; Coppi, A.; Sinnis, P.; Kim, K.
Attenuated Plasmodium yoelii lacking purine nucleoside phosphorylase confer protective immunity
Nat. Med.
14
954-958
2008
Plasmodium falciparum, Plasmodium yoelii
Ho, M.C.; Shi, W.; Rinaldo-Matthis, A.; Tyler, P.C.; Evans, G.B.; Clinch, K.; Almo, S.C.; Schramm, V.L.
Four generations of transition-state analogues for human purine nucleoside phosphorylase
Proc. Natl. Acad. Sci. USA
107
4805-4812
2010
Homo sapiens (P00491), Homo sapiens
Timmers, L.F.; Caceres, R.A.; Basso, L.A.; Santos, D.S.; De Azevedo, W.F.
Structural bioinformatics study of PNP from Listeria monocytogenes
Protein Pept. Lett.
15
843-849
2008
Listeria monocytogenes
Afshar, S.; Sawaya, M.R.; Morrison, S.L.
Structure of a mutant human purine nucleoside phosphorylase with the prodrug, 2-fluoro-2-deoxyadenosine and the cytotoxic drug, 2-fluoroadenine
Protein Sci.
18
1107-1114
2009
Homo sapiens (P00491), Homo sapiens
Aberg, A.M.; Ronquist, G.; Haney, M.; Waldenstrom, A.
Effects of some modulators on purine nucleoside phosphorylase activity in myocardial tissue
Scand. J. Clin. Lab. Invest.
70
8-14
2010
Sus scrofa
Eustaquio, A.S.; McGlinchey, R.P.; Liu, Y.; Hazzard, C.; Beer, L.L.; Florova, G.; Alhamadsheh, M.M.; Lechner, A.; Kale, A.J.; Kobayashi, Y.; Reynolds, K.A.; Moore, B.S.
Biosynthesis of the salinosporamide A polyketide synthase substrate chloroethylmalonyl-coenzyme A from S-adenosyl-L-methionine
Proc. Natl. Acad. Sci. USA
106
12295-12300
2009
Salinispora tropica (B0L7E7)
Martins, N.H.; Meza, A.N.; Santos, C.R.; de Giuseppe, P.O.; Murakami, M.T.
Molecular cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of a purine nucleoside phosphorylase from Bacillus subtilis strain 168
Acta Crystallogr. Sect. F
67
618-622
2011
Bacillus subtilis (O34925), Bacillus subtilis, Bacillus subtilis 168 (O34925)
Almendros, M.; Berenguer, J.; Sinisterra, J.
Thermus thermophilus nucleoside phosphorylases active in the synthesis of nucleoside analogues
Appl. Environ. Microbiol.
78
3128-3135
2012
Thermus thermophilus (Q72IR2), Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 (Q72IR2)
Vande Voorde, J.; Quintiliani, M.; McGuigan, C.; Liekens, S.; Balzarini, J.
Inhibition of pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative
Biochem. Pharmacol.
83
1358-1363
2012
Homo sapiens, Mycoplasma hyorhinis
Mikleusevic, G.; Stefanic, Z.; Narczyk, M.; Wielgus-Kutrowska, B.; Bzowska, A.; Luic, M.
Validation of the catalytic mechanism of Escherichia coli purine nucleoside phosphorylase by structural and kinetic studies
Biochimie
93
1610-1622
2011
Escherichia coli (P0ABP8), Escherichia coli
Zhu, S.; Ren, L.; Wang, J.; Zheng, G.; Tang, P.
Two-step efficient synthesis of 5-methyluridine via two thermostable nucleoside phosphorylase from Aeropyrum pernix
Bioorg. Med. Chem. Lett.
22
2102-2104
2012
Aeropyrum pernix
Wielgus-Kutrowska, B.; Breer, K.; Hashimoto, M.; Hikishima, S.; Yokomatsu, T.; Narczyk, M.; Dyzma, A.; Girstun, A.; Staro?, K.; Bzowska, A.
Trimeric purine nucleoside phosphorylase: exploring postulated one-third-of-the-sites binding in the transition state
Bioorg. Med. Chem.
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6758-6769
2012
Bos taurus
Xie, X.; Xia, J.; He, K.; Lu, L.; Xu, Q.; Chen, N.
Low-molecular-mass purine nucleoside phosphorylase: characterization and application in enzymatic synthesis of nucleoside antiviral drugs
Biotechnol. Lett.
33
1107-1112
2011
Bacillus subtilis, Escherichia coli, Pseudoalteromonas sp., Bacillus subtilis 168
Cobb, S.L.; Deng, H.; Hamilton, J.T.; McGlinchey, R.P.; O'Hagan, D.
Identification of 5-fluoro-5-deoxy-D-ribose-1-phosphate as an intermediate in fluorometabolite biosynthesis in Streptomyces cattleya
Chem. Commun. (Camb. )
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2004
Streptomyces cattleya
Luo, W.; Liu, Y.; Zhu, X.; Zhao, W.; Huang, L.; Cai, J.; Xu, Z.; Cen, P.
Cloning and characterization of purine nucleoside phosphorylase in Escherichia coli and subsequent ribavirin biosynthesis using immobilized recombinant cells
Enzyme Microb. Technol.
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2011
Escherichia coli (P0ABP8), Escherichia coli
Xie, X.; Huo, W.; Xia, J.; Xu, Q.; Chen, N.
Structure-activity relationship of a cold-adapted purine nucleoside phosphorylase by site-directed mutagenesis
Enzyme Microb. Technol.
51
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2012
Pseudoalteromonas sp. (C8CPR9), Pseudoalteromonas sp., Pseudoalteromonas sp. XM2107 (C8CPR9)
Cui, H.; Ruda, G.F.; Carrero-Lerida, J.; Ruiz-Perez, L.M.; Gilbert, I.H.; Gonzalez-Pacanowska, D.
Exploring new inhibitors of Plasmodium falciparum purine nucleoside phosphorylase
Eur. J. Med. Chem.
45
5140-5149
2010
Plasmodium falciparum (Q8I3X4), Plasmodium falciparum
Hassan, A.E.; Abou-Elkhair, R.A.; Riordan, J.M.; Allan, P.W.; Parker, W.B.; Khare, R.; Waud, W.R.; Montgomery, J.A.; Secrist, J.A.
Synthesis and evaluation of the substrate activity of C-6 substituted purine ribosides with E. coli purine nucleoside phosphorylase: palladium mediated cross-coupling of organozinc halides with 6-chloropurine nucleosides
Eur. J. Med. Chem.
47
167-174
2012
Escherichia coli
Stefanic, Z.; Narczyk, M.; Mikleusevic, G.; Wielgus-Kutrowska, B.; Bzowska, A.; Luic, M.
New phosphate binding sites in the crystal structure of Escherichia coli purine nucleoside phosphorylase complexed with phosphate and formycin A
FEBS Lett.
586
967-971
2012
Escherichia coli (P0ABP8), Escherichia coli
Szeker, K.; Niemitalo, O.; Casteleijn, M.G.; Juffer, A.H.; Neubauer, P.
High-temperature cultivation and 5 mRNA optimization are key factors for the efficient overexpression of thermostable Deinococcus geothermalis purine nucleoside phosphorylase in Escherichia coli
J. Biotechnol.
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268-274
2010
Deinococcus geothermalis
Rejman, D.; Panova, N.; Klener, P.; Maswabi, B.; Pohl, R.; Rosenberg, I.
N-phosphonocarbonylpyrrolidine derivatives of guanine: a new class of bi-substrate inhibitors of human purine nucleoside phosphorylase
J. Med. Chem.
55
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Homo sapiens
Nannemann, D.; Kaufmann, K.; Meiler, J.; Bachmann, B.
Design and directed evolution of a dideoxy purine nucleoside phosphorylase
Protein Eng. Des. Sel.
23
607-616
2010
Homo sapiens (P00491), Homo sapiens
Xie, X.; Wang, G.; Xia, J.; Chen, N.
Characterization of a recombinant cold-adapted purine nucleoside phosphorylase and its application in ribavirin bioconversion
World J. Microbiol. Biotechnol.
27
1175-1181
2011
Pseudoalteromonas sp. (C8CPR9), Pseudoalteromonas sp. XM2107 (C8CPR9)
Zhu, S.; Song, D.; Gong, C.; Tang, P.; Li, X.; Wang, J.; Zheng, G.
Biosynthesis of nucleoside analogues via thermostable nucleoside phosphorylase
Appl. Microbiol. Biotechnol.
97
6769-6778
2013
Aeropyrum pernix (Q9YDC0), Aeropyrum pernix, Aeropyrum pernix DSM 11879 (Q9YDC0)
Zhou, X.; Szeker, K.; Janocha, B.; Bhme, T.; Albrecht, D.; Mikhailopulo, I.A.; Neubauer P.
Recombinant purine nucleoside phosphorylases from thermophiles: preparation, properties and activity towards purine and pyrimidine nucleosides
FEBS J.
280
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Aeropyrum pernix (Q9YDC0), Aeropyrum pernix DSM 11879 (Q9YDC0)
Bagarolo, M.L.; Porcelli, M.; Martino, E.; Feller, G.; Cacciapuoti, G.
Multiple disulfide bridges modulate conformational stability and flexibility in hyperthermophilic archaeal purine nucleoside phosphorylase
Biochim. Biophys. Acta
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1458-1465
2015
Saccharolobus solfataricus (Q97W94), Saccharolobus solfataricus, Saccharolobus solfataricus P2 (Q97W94)
Stuer-Lauridsen, B.; Nygaard, P.
Purine salvage in two halophilic archaea: characterization of salvage pathways and isolation of mutants resistant to purine analogs
J. Bacteriol.
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457-463
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Halobacterium salinarum, Haloferax volcanii
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Development of a capillary electrophoresis method for analyzing adenosine deaminase and purine nucleoside phosphorylase and its application in inhibitor screening
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Homo sapiens
Wielgus-Kutrowska, B.; Modrak-Wojcik, A.; Dyzma, A.; Breer, K.; Zolkiewski, M.; Bzowska, A.
Purine nucleoside phosphorylase activity decline is linked to the decay of the trimeric form of the enzyme
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Bos taurus (P55859)
Fateev, I.V.; Antonov, K.V.; Konstantinova, I.D.; Muravyova, T.I.; Seela, F.; Esipov, R.S.; Miroshnikov, A.I.; Mikhailopulo, I.A.
The chemoenzymatic synthesis of clofarabine and related 2-deoxyfluoroarabinosyl nucleosides: the electronic and stereochemical factors determining substrate recognition by E. coli nucleoside phosphorylases
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Escherichia coli
Bromley, J.R.; Warnes, B.J.; Newell, C.A.; Thomson, J.C.; James, C.M.; Turnbull, C.G.; Hanke, D.E.
A purine nucleoside phosphorylase in Solanum tuberosum L. (potato) with specificity for cytokinins contributes to the duration of tuber endodormancy
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Solanum tuberosum
Isaksen, G.V.; Hopmann, K.H.; Aqvist, J.; Brandsdal, B.O.
Computer simulations reveal substrate specificity of glycosidic bond cleavage in native and mutant human purine nucleoside phosphorylase
Biochemistry
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Homo sapiens
Hassan, A.E.; Abou-Elkhair, R.A.; Parker, W.B.; Allan, P.W.; Secrist, J.A.
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Escherichia coli
Lopez-Cruz, R.I.; Perez-Milicua, M.B.; Crocker, D.E.; Gaxiola-Robles, R.; Bernal-Vertiz, J.A.; de la Rosa, A.; Vazquez-Medina, J.P.; Zenteno-Savin, T.
Purine nucleoside phosphorylase and xanthine oxidase activities in erythrocytes and plasma from marine, semiaquatic and terrestrial mammals
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Sus scrofa, Tursiops truncatus, Lontra longicaudis, Mirounga angustirostris
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Functional analysis of purine nucleoside phosphorylase as a key enzyme in ribavirin metabolism
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Homo sapiens
Donaldson, T.M.; Cassera, M.B.; Ho, M.C.; Zhan, C.; Merino, E.F.; Evans, G.B.; Tyler, P.C.; Almo, S.C.; Schramm, V.L.; Kim, K.
Inhibition and structure of Toxoplasma gondii purine nucleoside phosphorylase
Eukaryot. Cell
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Plasmodium falciparum, Toxoplasma gondii (Q2HXR2), Toxoplasma gondii
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Unique substrate specificity of purine nucleoside phosphorylases from Thermus thermophilus
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Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Bertosa, B.; Mikleusevic, G.; Wielgus-Kutrowska, B.; Narczyk, M.; Hajnic, M.; Lescic Asler, I.; Tomic, S.; Luic, M.; Bzowska, A.
Homooligomerization is needed for stability: a molecular modelling and solution study of Escherichia coli purine nucleoside phosphorylase
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Escherichia coli (P0ABP8), Escherichia coli
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Behavior of Plasmodium falciparum purine nucleoside phosphorylase in macromolecular crowded environment
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Plasmodium falciparum
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New insights into active site conformation dynamics of E. coli PNP revealed by combined H/D exchange approach and molecular dynamics simulations
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Escherichia coli
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Development of a new HPLC method using fluorescence detection without derivatization for determining purine nucleoside phosphorylase activity in human plasma
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Homo sapiens
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Isolation and substrate specificity of an adenine nucleoside phosphorylase from adult Schistosoma mansoni
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Schistosoma mansoni
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Site-selective ribosylation of fluorescent nucleobase analogs using purine-nucleoside phosphorylase as a catalyst: effects of point mutations
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Bos taurus
Donaldson, T.M.; Ting, L.M.; Zhan, C.; Shi, W.; Zheng, R.; Almo, S.C.; Kim, K.
Structural determinants of the 5-methylthioinosine specificity of Plasmodium purine nucleoside phosphorylase
PLoS ONE
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Plasmodium falciparum (Q8T9Z7), Plasmodium falciparum
Suarez, J.; Schramm, V.L.
Isotope-specific and amino acid-specific heavy atom substitutions alter barrier crossing in human purine nucleoside phosphorylase
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Homo sapiens
Roca, M.; Moliner, V.; Tunon, I.
Origin of enzymatic kinetic isotope effects in human purine nucleoside phosphorylase
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Homo sapiens (P00491)
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Timofeev, V.I.; Zhukhlistova, N.E.; Abramchik, Y.A.; Muravieva, T.I.; Esipov, R.S.; Kuranova, I.P.
Crystal structure of Escherichia coli purine nucleoside phosphorylase complexed with acyclovir
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Escherichia coli (P0ABP8), Escherichia coli
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Development, validation and application of a 96-well enzymatic assay based on LC-ESI-MS/MS quantification for the screening of selective inhibitors against Mycobacterium tuberculosis purine nucleoside phosphorylase
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Mycobacterium tuberculosis, Homo sapiens (P00491), Homo sapiens
Isaksen, G.V.; Aqvist, J.; Brandsdal, B.O.
Thermodynamics of the purine nucleoside phosphorylase reaction revealed by computer simulations
Biochemistry
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Homo sapiens (P00491), Homo sapiens
Kamel, S.; Thiele, I.; Neubauer, P.; Wagner, A.
Thermophilic nucleoside phosphorylases their properties, characteristics and applications
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Parageobacillus thermoglucosidasius, Deinococcus geothermalis, Aeropyrum pernix (A0A401H9D3), Geobacillus stearothermophilus (P77834), Geobacillus stearothermophilus (P77835), Thermus thermophilus (Q72IR2), Thermus thermophilus (Q72L69), Pyrococcus furiosus (Q8U2I1), Pyrococcus furiosus ATCC 43587 (Q8U2I1), Thermus thermophilus DSM 7039 (Q72IR2), Thermus thermophilus DSM 7039 (Q72L69), Pyrococcus furiosus Vc1 (Q8U2I1), Deinococcus geothermalis DSM 11300, Deinococcus geothermalis AG-3a, Parageobacillus thermoglucosidasius 11955, Pyrococcus furiosus JCM 8422 (Q8U2I1), Thermus thermophilus ATCC BAA-163 (Q72IR2), Thermus thermophilus ATCC BAA-163 (Q72L69)
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A synergistic effect of phosphate, pH and Phe159 substitution on the formycin A association to the E. coli purine nucleoside phosphorylase
Biochimie
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Escherichia coli (P0ABP8), Escherichia coli
Timofeev, V.; Abramchik, Y.; Zhukhlistova, N.; Muravieva, T.; Esipov, R.; Kuranova, I.
Three-dimensional structure of E. coli purine nucleoside phosphorylase at 0.99 A resolution
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Escherichia coli (P0ABP8)
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Wierzchowski, J.; Stachelska-Wierzchowska, A.; Wielgus-Kutrowska, B.; Bzowska, A.
1,N6-ethenoadenine and other fluorescent nucleobase analogs as substrates for purine-nucleoside phosphorylases Spectroscopic and kinetic studies
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Bacillus cereus, Pectobacterium carotovorum, Thermus thermophilus, Plasmodium lophurae, Helicobacter pylori (A0A518Y5Z2), Homo sapiens (P00941), Escherichia coli (P0ABP8), Escherichia coli (P45563), Bos taurus (P55859), Cellulomonas sp. (P81989), Plasmodium falciparum (Q8I3X4)
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Helicobacterpylori purine nucleoside phosphorylase shows new distribution patterns of open and closed active site conformations and unusual biochemical features
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Helicobacter pylori (P56463), Helicobacter pylori ATCC 700392 (P56463), Helicobacter pylori 26695 (P56463)
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Structural characterization of purine nucleoside phosphorylase from human pathogen Helicobacter pylori
Int. J. Biol. Macromol.
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Helicobacter pylori (A0A0G2R4X7), Helicobacter pylori
Wen, Q.H.; Wang, L.H.; Zeng, X.A.; Niu, D.B.; Wang, M.S.
Hydroxyl-related differences for three dietary flavonoids as inhibitors of human purine nucleoside phosphorylase
Int. J. Biol. Macromol.
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Homo sapiens (P00491), Homo sapiens
Kagami, L.P.; das Neves, G.M.; Rodrigues, R.P.; da Silva, V.B.; Eifler-Lima, V.L.; Kawano, D.F.
Identification of a novel putative inhibitor of the Plasmodium falciparum purine nucleoside phosphorylase exploring the purine salvage pathway to design new antimalarial drugs
Mol. Divers.
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Plasmodium falciparum (Q8I3X4), Plasmodium falciparum
Stachelska-Wierzchowska, A.; Wierzchowski, J.; Bzowska, A.; Wielgus-Kutrowska, B.
Site-selective ribosylation of fluorescent nucleobase analogs using purine-nucleoside phosphorylase as a catalyst effects of point mutations
Molecules
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Bos taurus (P55859), Escherichia coli (P0ABP8), Escherichia coli, Homo sapiens (P00491), Homo sapiens
Stachelska-Wierzchowska, A.; Wierzchowski, J.; Gorka, M.; Bzowska, A.; Wielgus-Kutrowska, B.
Tri-cyclic nucleobase analogs and their ribosides as substrates of purine-nucleoside phosphorylases II guanine and isoguanine derivatives
Molecules
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Escherichia coli (P45563), Escherichia coli
Liu, G.; Cheng, T.; Chu, J.; Li, S.; He, B.
Efficient synthesis of purine nucleoside analogs by a new trimeric purine nucleoside phosphorylase from Aneurinibacillus migulanus AM007
Molecules
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Aneurinibacillus migulanus (A0A3G5E8S8), Aneurinibacillus migulanus, Aneurinibacillus migulanus AM007 (A0A3G5E8S8)
Stachelska-Wierzchowska, A.; Wierzchowski, J.; Bzowska, A.; Wielgus-Kutrowska, B.
Tricyclic nitrogen base 1,N6-ethenoadenine and its ribosides as substrates for purine-nucleoside phosphorylases spectroscopic and kinetic studies
Nucleosides Nucleotides Nucleic Acids
37
89-101
2018
Escherichia coli (P0ABP8), Escherichia coli
Hayat, F.; Migaud, M.E.
Nicotinamide riboside-amino acid conjugates that are stable to purine nucleoside phosphorylase
Org. Biomol. Chem.
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2877-2885
2020
Homo sapiens (P00491)
Mahor, D.; Priyanka, A.; Prasad, G.S.; Thakur, K.G.
Functional and structural characterization of purine nucleoside phosphorylase from Kluyveromyces lactis and its potential applications in reducing purine content in food
PLoS ONE
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e0164279
2016
Kluyveromyces lactis (Q6CSZ6), Kluyveromyces lactis, Kluyveromyces lactis CBS 2359 (Q6CSZ6), Kluyveromyces lactis NRRL Y-1140 (Q6CSZ6), Kluyveromyces lactis DSM 70799 (Q6CSZ6), Kluyveromyces lactis WM37 (Q6CSZ6), Kluyveromyces lactis ATCC 8585 (Q6CSZ6), Kluyveromyces lactis NBRC 1267 (Q6CSZ6)
Harijan, R.K.; Zoi, I.; Antoniou, D.; Schwartz, S.D.; Schramm, V.L.
Catalytic-site design for inverse heavy-enzyme isotope effects in human purine nucleoside phosphorylase
Proc. Natl. Acad. Sci. USA
114
6456-6461
2017
Homo sapiens (P00491), Homo sapiens
Jackson, E.K.; Gillespie, D.G.; Cheng, D.; Mi, Z.; Menshikova, E.V.
Characterization of the N6-etheno-bridge method to assess extracellular metabolism of adenine nucleotides detection of a possible role for purine nucleoside phosphorylase in adenosine metabolism
Purinergic Signal.
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2020
Homo sapiens (P00491), Rattus norvegicus (P85973), Rattus norvegicus Wistar-Kyoto (P85973)
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