Any feedback?
No. of entries
Information on EC 1.7.3.1 - nitroalkane oxidase
for references in articles please use BRENDA:EC1.7.3.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 1 Oxidoreductases
1.7 Acting on other nitrogenous compounds as donors
1.7.3 With oxygen as acceptor
1.7.3.1 nitroalkane oxidase
IUBMB Comments
Has an absolute requirement for FAD . While nitroethane may be the physiological substrate , the enzyme also acts on several other nitroalkanes, including 1-nitropropane, 2-nitropropane, 1-nitrobutane, 1-nitropentane, 1-nitrohexane, nitrocyclohexane and some nitroalkanols . Differs from EC 1.13.11.16, nitronate monooxygenase, in that the preferred substrates are neutral nitroalkanes rather than anionic nitronates .
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
- 1.7.3.1
-
atlantic
-
oscillation
-
climate
-
winter
-
atmospheric
- orange
-
robot
-
weather
- acridine
-
ocean
-
interannual
-
summer
-
arctic
- nai
-
phenology
- cardiolipin
-
humanoid
-
forecast
-
warmer
-
nonyl
-
rainfall
-
meteorological
-
hydrological
-
seabird
-
snow
-
latitudinal
showSynonyms
nao, nitroalkane oxidase, pa4202, nitroalkane-oxidizing enzyme, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
NAO
nitroalkane-oxidizing enzyme
nitroethane oxidase
nitroethane:oxygen oxidoreductase
-
-
-
-
NOE
oxidase, nitroethane
-
-
-
-
additional information
nitroethane oxidase
-
-
-
-
additional information
-
enzyme belongs to the acyl-CoA dehydrogenase family of enzymes
additional information
-
enzyme belongs to the acyl-CoA dehydrogenase family of enzymes
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O
-
-
-
-
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O
mechanism
-
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O
ping pong kinetic mechanism
-
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O
Asp402 acts as the active site base of the enzyme, mechanism, substrate CH-bond cleavage is rate limiting for the reduction of the enzyme by nitroethane
-
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O
Asp402 acts as the active site base of the enzyme, mechanism: catalysis is initiated by the active site base Asp402 that deprotonates the neutral substrate to yield a carbanion that can attack the N-5 position of the oxidized flavin, CH-bond cleavage is rate limiting for the reduction of the enzyme by nitroethane
-
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O
detailed mechanism, with carbanion reaction intermediate formation, an aspartate residue is the active site base, substrate CH-bond cleavage is rate limiting for the reduction of the enzyme by nitroethane, the active site of the enzyme is a hydrophobic channel, tunneling, overview
-
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O
mechanism, involves removal of a proton from the nitroalkane, forming a carbanion which adds to the N5 of the flavin, elimination of nitrite from the resulting adduct forms an electrophilic imine intermediate which can be attacked by hydroxide
-
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O
mechanism, transition-state stabilization is essential, protein-substrate interactions in the transition state, reaction involves quantum mechaniscal tunneling, Asp402 is involved in initial abstraction of a proton from the neutral substrate, overview
-
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O
critical importance of the interaction between Asp402 and Arg409 for proton abstraction by nitroalkane oxidase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
nitroalkane:oxygen oxidoreductase
Has an absolute requirement for FAD [4]. While nitroethane may be the physiological substrate [2], the enzyme also acts on several other nitroalkanes, including 1-nitropropane, 2-nitropropane, 1-nitrobutane, 1-nitropentane, 1-nitrohexane, nitrocyclohexane and some nitroalkanols [4]. Differs from EC 1.13.11.16, nitronate monooxygenase, in that the preferred substrates are neutral nitroalkanes rather than anionic nitronates [4].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9029-36-1
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-nitroalkane + O2 + H2O
aldehyde + H2O2 + nitrite
-
Substrates: -
Products: -
Products: -
?
1-nitrobutane + H2O + O2
butyraldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
1-nitrohexane + O2 + H2O
hexanaldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
1-nitrooctane + O2 + H2O
octanal + nitrite + H2O2
Substrates: -
Products: -
Products: -
?
1-nitropentane + O2
pentanaldehyde + HNO2
-
Substrates: -
Products: -
Products: -
?
1-nitropropane + H2O
propanal + nitrite + H2O2
Substrates: -
Products: -
Products: -
?
1-nitropropane + O2
propionaldehyde + HNO2
-
Substrates: -
Products: -
Products: -
?
2-nitropropane + H2O
propanone + nitrite + H2O2
Substrates: -
Products: -
Products: -
?
3-nitropropanoic acid + H2O + O2
2-oxopropanoic acid + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
nitroalkane + O2 + H2O
aldehyde or ketone + nitrite + H2O2
-
Substrates: neutral nitroalkanes
Products: -
Products: -
?
nitroethane + H2O
acetaldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
nitroethane + H2O + O2
ethanal + nitrite + H2O2
Substrates: -
Products: -
Products: -
?
nitroethane + O2 + H2O
acetaldehyde + nitrite + H2O2
Substrates: -
Products: -
Products: -
?
phenylnitromethane + O2 + H2O
? + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
phenylnitromethane + O2 + H2O
phenylaldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
1-nitrobutane + O2 + H2O
butyraldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
1-nitrobutane + O2 + H2O
butyraldehyde + nitrite + H2O2
-
Substrates: 400fold more effective than nitroethane
Products: -
Products: -
?
1-nitrohexane + O2 + H2O
hexanal + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
1-nitrohexane + O2 + H2O
hexanal + nitrite + H2O2
Substrates: -
Products: -
Products: -
?
1-nitropropane + H2O + O2
propionaldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
1-nitropropane + H2O + O2
propionaldehyde + nitrite + H2O2
Substrates: -
Products: -
Products: -
?
1-nitropropane + H2O + O2
propionaldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
1-nitropropane + H2O + O2
propionaldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
1-nitropropane + H2O + O2
propionaldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
1-nitropropane + H2O + O2
propionaldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
2-nitropropane + H2O + O2
acetone + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
2-nitropropane + H2O + O2
acetone + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
2-nitropropane + H2O + O2
acetone + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
nitroethane + H2O
ethanal + nitrite + H2O2
Substrates: solvent isotope and viscositiy effects: the kcat proton inventory is consistent with a single exchangeable proton in flight, while the kcat/KM is consistent with either a single proton in flight in the transition state or a medium effect. Increasing the solvent viscosity does not affect the kcat or kcat/KM value significantly, establishing that nitroethane binding is at equilibrium and that product release does not limit kcat
Products: -
Products: -
?
nitroethane + H2O
ethanal + nitrite + H2O2
Substrates: solvent isotope and viscositiy effects: the kcat proton inventory is consistent with a single exchangeable proton in flight, while the kcat/KM is consistent with either a single proton in flight in the transition state or a medium effect. Increasing the solvent viscosity does not affect the kcat or kcat/KM value significantly, establishing that nitroethane binding is at equilibrium and that product release does not limit kcat
Products: -
Products: -
?
nitroethane + H2O
ethanal + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
nitroethane + H2O
ethanal + nitrite + H2O2
Substrates: -
Products: -
Products: -
?
nitroethane + H2O + O2
acetaldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
nitroethane + H2O + O2
acetaldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
nitroethane + O2
acetaldehyde + HNO2
-
Substrates: production of H2O2
Products: -
Products: -
?
nitroethane + O2
acetaldehyde + HNO2
-
Substrates: at pH 7, bi-ter ping-pong mechanism with oxygen reacting with the free reduced enzyme after release of the aldehyde product
Products: -
Products: -
?
nitroethane + O2 + H2O
ethanal + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
nitroethane + O2 + H2O
ethanal + nitrite + H2O2
-
Substrates: nitroethane is the only substrate, of diverse primary and secondary alkanes, for which deprotonation is fully rate limiting for the reductive half reaction
Products: -
Products: -
?
nitroethane + O2 + H2O
ethanal + nitrite + H2O2
-
Substrates: O2 is delivered from air-saturated buffer in the assay reaction
Products: -
Products: -
?
nitroethane + O2 + H2O
ethanal + nitrite + H2O2
-
Substrates: substrate CH-bond cleavage is rate limiting for the reduction of the enzyme by nitroethane
Products: -
Products: -
?
nitroethane + O2 + H2O
ethanal + nitrite + H2O2
-
Substrates: the neutral substrate form is preferred, 5fold lower activity with the substrate anion, the D402 mutant enzymes in contrast prefer the anionic substrate form
Products: -
Products: -
?
nitroethane + O2 + H2O
ethanal + nitrite + H2O2 + H+
-
Substrates: -
Products: -
Products: -
?
nitroethane + O2 + H2O
ethanal + nitrite + H2O2 + H+
Substrates: -
Products: -
Products: -
ir
additional information
?
-
-
Substrates: the physiological role of the enzyme is the oxidation of nitroaliphatic species
Products: -
Products: -
?
additional information
?
-
-
Substrates: analysis of reaction intermediates formed by mass spectrometry and gel filtration
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme catalyzes the oxidation of nitroalkanes to the respective aldehydes or ketones with consumption of molecular oxygen and releasing nitrite and hydrogen peroxide
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme catalyzes the oxidation of primary and secondary nitroalkanes to the respective aldehydes or ketones, releasing nitrite
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme does not require anionic nitroalkanes for activity, enzyme catalyzes the oxidation of a number of primary and secondary nitroalkanes to the respective aldehydes or ketones with preference for primary nitroalkanes, monotonic increase with each additional methylene group from nitroethane to 1-nitrobutane increasing kcat/Km value by 20fold, no further increase with 1-nitropentane and 1-nitrohexane
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme shows high substrate specificity
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme does not oxidize acyl-CoA substrates and prefers primary nitroalkanes containing four or more carbon atoms
Products: -
Products: -
-
additional information
?
-
-
Substrates: slight activity, less than 5% of the activity towards nitroethane with: nitromethane, nitroacetic acid, 1-chloro-1-nitropropane, methylene blue also can function as hydrogen acceptor with low reaction rate
Products: -
Products: -
?
additional information
?
-
Substrates: involved in the conversion of toxic nitroalkanes to less harmful compounds
Products: -
Products: -
?
additional information
?
-
-
Substrates: involved in the conversion of toxic nitroalkanes to less harmful compounds
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme prefers the neutral forms of substrates and only reacts with anionic 2-nitropropane at a low rate
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme prefers the neutral forms of substrates and only reacts with anionic 2-nitropropane at a low rate
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
nitroethane + H2O + O2
acetaldehyde + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
nitroethane + O2 + H2O
ethanal + nitrite + H2O2
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: the physiological role of the enzyme is the oxidation of nitroaliphatic species
Products: -
Products: -
?
additional information
?
-
Substrates: involved in the conversion of toxic nitroalkanes to less harmful compounds
Products: -
Products: -
?
additional information
?
-
-
Substrates: involved in the conversion of toxic nitroalkanes to less harmful compounds
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FAD
-
FAD in the recombinant enzyme is in the oxidized state, with a spectrum indistinguishable from that of the activated form of the nonrecombinant enzyme
FAD
-
1 mol of FAD per mol of subunit is required for catalysis, reversible removal of FAD yields inactive enzyme
FAD
-
absolutely required, binding structure, involving Ser171, and reactive structure analysis, the apoenzyme is inactive, removal of cofactor in its active form is reversible
FAD
-
formation of 5-nitroalkyl-FAD intermediates, structure in cyanide inactivated enzyme
FMN
-
dependent on. The 2-nitropropane conversion efficiency of the crude cell extract increases by 2-3 fold when added with FMN
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
acetaldehyde
-
noncompetitive inhibitor versus nitroethane due to formation of a dead-end complex between the oxidized enzyme and the product, competitive inhibitor versus oxygen
Butyraldehyde
-
noncompetitive inhibitor versus nitroethane due to formation of a dead-end complex between the oxidized enzyme and the product
cyanide
-
inactivation of the enzyme by trapping of the electrophilic imine reaction intermediate, formation of a cyanoalkyl intermediate, reaction with the substrate after proton abstraction but before flavin oxidation
nitroethane
-
substrate inhibition detected in mutant enzyme R409K, no substrate inhibition is detected in mutant enzyme D402E
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
imidazole
-
unprotonated imidazole causes a 5fold increase in maximal velocity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Acute Coronary Syndrome
Long-term treatment with NAO in acute coronary syndromes: rationale and clinical data.
Adenoma
Fine needle aspiration diagnosis of hyperplastic and neoplastic follicular nodules of the thyroid. A morphometric study.
Alzheimer Disease
Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease.
Asthma
Irreversible airway obstruction assessed by high-resolution computed tomography (HRCT), exhaled nitric oxide (FENO), and biological markers in induced sputum in patients with asthma.
Asthma
[Determination and comparison of pulmonary function with anthropometric indexes in asthmatic and non-asthmatic obese teenagers]
Atrial Fibrillation
High CHA2DS2-VASc score without atrial fibrillation: 'NAO yes, NAO no'.
Brain Ischemia
Effect of Pei Yuan Tong Nao capsules on neuronal function and metabolism in cerebral ischemic rats.
Brain Ischemia
[Effect of nao shuan tong on the activity of SOD in cerebral tissues and NO in serums in the cerebral ischemia rats].
Brain Ischemia
[Influence of yi nao capsule on epinephrine and norepinephrine during rat ischemia].
Carcinoma
Fine needle aspiration diagnosis of hyperplastic and neoplastic follicular nodules of the thyroid. A morphometric study.
Cerebral Hemorrhage
Induction of NADPH-diaphorase activity in the forebrain in a model of intracerebral hemorrhage and its inhibition by the traditional Chinese medicine complex Nao Yi An.
Cerebral Hemorrhage
[Effects of plasma catecholamines contents on acute cerebral hemorrhage treated with nao yi-an granule]
Cerebral Palsy
A motor learning therapeutic intervention for a child with cerebral palsy through a social assistive robot.
Cerebrovascular Disorders
The pharmacokinetic screening of multiple components of the Nao Mai Tong formula in rat plasma by liquid chromatography tandem mass spectrometry combined with pattern recognition method and its application to comparative pharmacokinetics.
Chickenpox
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Cleft Lip
Computational Analysis of the Mature Unilateral Cleft Lip Nasal Deformity on Nasal Patency.
Communicable Diseases
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Congenital Abnormalities
Computational Analysis of the Mature Unilateral Cleft Lip Nasal Deformity on Nasal Patency.
Contracture
Submaximal sodium-lack contractures in rapidly perfused frog ventricular strips.
COVID-19
Covid-19: Poor links between NHS and social care weakened England's response, says NAO.
COVID-19
Covid-19: Test and trace system must improve its below par performance, NAO concludes.
Cysts
Dinoflagellate cysts and hydrographical change in Gullmar Fjord, west coast of Sweden.
Cysts
Hundred years of environmental change and phytoplankton ecophysiological variability archived in coastal sediments.
Dementia
Tong Luo Jiu Nao ameliorates A?1-40-induced cognitive impairment on adaptive behavior learning by modulating ERK/CaMKII/CREB signaling in the hippocampus.
Dementia, Multi-Infarct
Jian Nao Ning for treatment of memory impairment in patients with mild or moderate multi-infarct dementia.
Dementia, Vascular
Effects of a traditional Chinese medicine, Qing Nao Yi Zhi Fang, on glutamate excitotoxicity in rat fetal cerebral neuronal cells in primary culture.
Dementia, Vascular
The diverse effects of a Chinese medicine, Qing Nao Yi Zhi Fang, on the proliferation of human arterial smooth muscle cells and endothelial cells.
Dementia, Vascular
Tong Luo Jiu Nao injection, a traditional Chinese medicinal preparation, inhibits MIP-1? expression in brain microvascular endothelial cells injured by oxygen-glucose deprivation.
Dysentery, Bacillary
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Encephalitis, Tick-Borne
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Encephalitis, Viral
Evaluation of the adjunctive effect of Xing Nao Jing Injection for viral encephalitis: A systematic review and meta-analysis of randomized controlled trials.
Endotoxemia
Effect of natural antioxidants and apocynin on LPS-induced endotoxemia in rabbit.
Endotoxemia
Effects of apocynin and natural antioxidant from spinach on inducible nitric oxide synthase and cyclooxygenase-2 induction in lipopolysaccharide-induced hepatic injury in rat.
Endotoxemia
The prophylactic effects of natural water-soluble antioxidant from spinach and apocynin in a rabbit model of lipopolysaccharide-induced endotoxemia.
Erysipelas
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Hajdu-Cheney Syndrome
A novel matrix metalloproteinase 2 (MMP2) terminal hemopexin domain mutation in a family with multicentric osteolysis with nodulosis and arthritis with cardiac defects.
Hemorrhagic Fever with Renal Syndrome
Time Series Analysis Performed on Nephropathia Epidemica in Humans of Northern Sweden in Relation to Bank Vole Population Dynamic and the NAO Index.
Hemorrhagic Stroke
[Effects of nao yi-an granule on hemorheological indexes and RCD (erythrocyte deformability) in patients with hemorrhagic stroke]
Hepatitis A
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Infectious Mononucleosis
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Ischemic Stroke
Tong Luo Jiu Nao injection, a traditional Chinese medicinal preparation, inhibits MIP-1? expression in brain microvascular endothelial cells injured by oxygen-glucose deprivation.
Joint Diseases
A novel matrix metalloproteinase 2 (MMP2) terminal hemopexin domain mutation in a family with multicentric osteolysis with nodulosis and arthritis with cardiac defects.
Leptospirosis
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Lyme Disease
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Measles
A link between the North Atlantic Oscillation and measles dynamics during the vaccination period in England and Wales.
Mumps
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Nasal Obstruction
Computational Analysis of the Mature Unilateral Cleft Lip Nasal Deformity on Nasal Patency.
Nasal Obstruction
Computed nasal resistance compared with patient-reported symptoms in surgically treated nasal airway passages: A preliminary report.
Nasal Obstruction
Correlation between Subjective Nasal Patency and Intranasal Airflow Distribution.
Nasal Obstruction
Diagnostic and Therapeutic Management of Nasal Airway Obstruction: Advances in Diagnosis and Treatment.
Nasal Obstruction
Endoscopic septoplasty: revisitation of the technique, indications, and outcomes.
Nasal Obstruction
The impact of nasal adhesions on airflow and mucosal cooling - A computational fluid dynamics analysis.
Nasal Obstruction
The Impact of Nasal Obstruction and Functional Septorhinoplasty on Sleep Quality.
Neoplasms
Enhanced Cytotoxic Activity of Mitochondrial Mechanical Effectors in Human Lung Carcinoma H520 Cells: Pharmaceutical Implications for Cancer Therapy.
Occupational Diseases
Features of Occupational Health Risks in the Russian Arctic (on the Example of Nenets Autonomous Okrug and Chukotka Autonomous Okrug).
Overweight
PROCEED: Prospective Obesity Cohort of Economic Evaluation and Determinants: baseline health and healthcare utilization of the US sample.
Papilloma
Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model.
Plague
The delayed effect of cooling reinforced the NAO-plague connection in pre-industrial Europe.
Renal Insufficiency
[Oliguric and non-oliguric renal failure in high risk patient in intensive care units (author's transl)]
Rhinitis
Correlations between computational fluid dynamics and clinical evaluation of nasal airway obstruction due to septal deviation: An observational study.
Salmonella Infections
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Scrub Typhus
[Study on the characteristics of Tsutsugamushi disease in the epidemic areas of south islands in China]
Sepsis
The prophylactic effects of natural water-soluble antioxidant from spinach and apocynin in a rabbit model of lipopolysaccharide-induced endotoxemia.
Sinusitis
Correlations between computational fluid dynamics and clinical evaluation of nasal airway obstruction due to septal deviation: An observational study.
Starvation
Unique natural antioxidants (NAOs) and derived purified components inhibit cell cycle progression by downregulation of ppRb and E2F in human PC3 prostate cancer cells.
Stroke
[Achievements and enlightenment of modern acupuncture therapy for stroke based on the neuroanatomy].
Surgical Wound Infection
Communicable disease and infection control: the surveillance contribution.
Thymoma
Cell quality control mechanisms maintain stemness and differentiation potential of P19 embryonic carcinoma cells.
Toxoplasmosis
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Tularemia
North Atlantic weather oscillation and human infectious diseases in the Czech Republic, 1951-2003.
Vascular Diseases
Effect of Pei Yuan Tong Nao capsules on neuronal function and metabolism in cerebral ischemic rats.
Vitamin E Deficiency
Vitamin E deficiency impairs the modifications of mitochondrial membrane potential and mass in rat splenocytes stimulated to proliferate.
Warts
Evaluation of mitochondrial content and activity with nonyl-acridine orange and rhodamine 123: flow cytometric analysis and comparison with quantitative morphometry. Comparative analysis by flow cytometry and quantitative morphometry of mitochondrial content and activity.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5.8
1-Nitropropane
-
wild type enzyme, at 30°C, pH not specified in the publication
9.6
1-Nitropropane
-
mutant enzyme H183S, at 30°C, pH not specified in the publication
9.8
1-Nitropropane
-
mutant enzyme H183C, at 30°C, pH not specified in the publication
1.9
nitroethane
-
mutant enzyme S171V, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C
2
nitroethane
-
mutant enzyme C397S, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C
2
nitroethane
-
mutant enzyme S171T, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C
2.1
nitroethane
-
mutant enzyme S171A, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C
2.3
nitroethane
-
wild type enzyme, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C
4.8
nitroethane
-
mutant enzyme Y398F, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C
5.4
nitroethane
-
wild type enzyme, at 30°C, pH not specified in the publication
8.1
nitroethane
-
mutant enzyme H183S, at 30°C, pH not specified in the publication
8.4
nitroethane
-
mutant enzyme H183C, at 30°C, pH not specified in the publication
0.011
O2
-
mutant enzyme S171T, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C
0.028
O2
-
mutant enzyme Y398F, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C
0.029
O2
-
mutant enzyme S171A, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C
0.044
O2
-
mutant enzyme S171V, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C
0.058
O2
-
mutant enzyme C397S, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C
additional information
additional information
-
kinetic competency of the cationic imine enzyme intermediate
-
additional information
additional information
-
kinetics and thermodynamics of the deprotonation step with substrate nitroethane, effects of tunneling
-
additional information
additional information
-
steady-state and rapid reaction kinetics of wild-type and mutant enzymes
-
additional information
additional information
-
thermodynamics, kinetic mechanism with nitroethane and 1-nitrobutane, isotopic effects, monotonic increase with each additional methylene group from nitroethane to 1-nitrobutane increasing kcat/Km value by 20fold, no further increase with 1-nitropentane and 1-nitrohexane
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE