Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

Information on EC 1.20.4.1 - arsenate reductase (glutathione/glutaredoxin)

for references in articles please use BRENDA:EC1.20.4.1

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
IUBMB Comments

The enzyme is part of a system for detoxifying arsenate. The substrate binds to a catalytic cysteine residue, forming a covalent thiolate—As(V) intermediate. A tertiary intermediate is then formed between the arsenic, the enzyme’s cysteine, and a glutathione cysteine. This intermediate is reduced by glutaredoxin, which forms a dithiol with the glutathione, leading to the dissociation of arsenite. Thus reduction of As(V) is mediated by three cysteine residues: one in ArsC, one in glutathione, and one in glutaredoxin. Although the arsenite formed is more toxic than arsenate, it can be extruded from some bacteria by EC 7.3.2.7, arsenite-transporting ATPase; in other organisms, arsenite can be methylated by EC 2.1.1.137, arsenite methyltransferase, in a pathway that produces non-toxic organoarsenical compounds. cf. EC 1.20.4.4, arsenate reductase (thioredoxin).

The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes

Synonyms
arsenate reductase, asv reductase, acr2p, pvgrx5, slr0946, synarsc, all0195, pcacr2, mxan_0575, more

REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
arsenate + glutathione + glutaredoxin = arsenite + a glutaredoxin-glutathione disulfide + H2O
show the reaction diagram
PATHWAY SOURCE
PATHWAYS
MetaCyc
arsenate detoxification I, arsenate detoxification III, arsenic detoxification (plants), arsenic detoxification (yeast)
Highest Expressing Human Cell Lines
Cell Line Links Gene Links