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IUBMB CommentsThe enzyme from the sulfate-reducing bacterium Desulfovibrio vulgaris can also act on (R)-2-hydroxybutanoate.
The enzyme appears in viruses and cellular organisms
Synonyms
d-lactate:ferricytochrome c-553 oxidoreductase, more
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D-lactate:ferricytochrome C-553 oxidoreductase
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(R)-lactate + 2 ferricytochrome c-553 = pyruvate + 2 ferrocytochrome c-553 + 2 H+
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MetaCyc
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales)
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(R)-lactate:ferricytochrome-c-553 2-oxidoreductase
The enzyme from the sulfate-reducing bacterium Desulfovibrio vulgaris can also act on (R)-2-hydroxybutanoate.
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D-lactate + 1-methoxyphenazinium methyl sulfate
pyruvate + ?
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Substrates: -
Products: -
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D-lactate + 2,6-dichlorophenolindophenol
pyruvate + ?
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Substrates: -
Products: -
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D-lactate + 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride
pyruvate + ?
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Substrates: -
Products: -
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D-lactate + ferricyanide
pyruvate + ferrocyanide
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Substrates: -
Products: -
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D-lactate + ferricytochrome c-553
pyruvate + ferrocytochrome c-553
D-lactate + methylene blue
pyruvate + ?
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Substrates: -
Products: -
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D-lactate + nitrotetrazolium blue
pyruvate + ?
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Substrates: -
Products: -
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DL-2-hydroxybutyrate + ferricytochrome c-553
2-ketobutyrate + ferrocytochrome c-553
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Substrates: -
Products: -
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additional information
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Substrates: no activity with: NAD+, NADP+, FAD, FMN, cytochrome C3 and O2. Enzyme does not have any diaphorase activity
Products: -
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D-lactate + ferricytochrome c-553
pyruvate + ferrocytochrome c-553
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Substrates: -
Products: -
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D-lactate + ferricytochrome c-553
pyruvate + ferrocytochrome c-553
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Substrates: starting enzyme for carbon and energy metabolism
Products: -
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D-lactate + ferricytochrome c-553
pyruvate + ferrocytochrome c-553
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Substrates: starting enzyme for carbon and energy metabolism
Products: -
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Ferricytochrome c-553
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EDTA
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slight stimulation
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2.4
DL-2-hydroxybutyrate
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8
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D-lactate + nitrotetrazolium blue
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7 - 9
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pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity, D-lactate + nitrotetrazolium blue
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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K8D4N3_CROSK
432
0
49133
TrEMBL
-
A0A0A2BN63_9CYAN
566
0
64311
TrEMBL
-
A0A090P4V6_9VIBR
363
0
40002
TrEMBL
-
W1F514_ECOLX
58
0
6773
TrEMBL
-
A0A484RF94_9ZZZZ
578
0
63200
TrEMBL
other Location (Reliability: 4)
A0A484RPB2_9ZZZZ
597
0
64980
TrEMBL
Mitochondrion (Reliability: 5)
K8AWU5_9ENTR
432
0
49022
TrEMBL
-
A0A484Q5J5_9ZZZZ
597
0
64950
TrEMBL
Mitochondrion (Reliability: 5)
A0A484RW93_9ZZZZ
597
0
64963
TrEMBL
Mitochondrion (Reliability: 5)
W1F4Y6_ECOLX
288
0
31429
TrEMBL
-
A0A1X6WU70_9MICO
320
0
34297
TrEMBL
-
A0A0A2BHD9_9CYAN
566
0
64246
TrEMBL
-
A0A484TZ80_9ZZZZ
599
0
65223
TrEMBL
Mitochondrion (Reliability: 2)
A0A6S6U9W4_9GAMM
116
0
12827
TrEMBL
-
W1F6L0_ECOLX
252
0
29347
TrEMBL
-
A0A484UDF8_9ZZZZ
597
0
65010
TrEMBL
Mitochondrion (Reliability: 5)
A0A484SHE4_9ZZZZ
578
0
63216
TrEMBL
other Location (Reliability: 4)
K8CRY5_CROSK
411
0
47057
TrEMBL
-
A0A1X6WUD8_9MICO
261
0
29069
TrEMBL
-
A0A7G2IKC5_CITFR
89
0
10530
TrEMBL
-
A0A484QF48_9ZZZZ
578
0
63231
TrEMBL
other Location (Reliability: 4)
A0A7G2IL42_CITFR
451
0
50756
TrEMBL
-
A0A090P548_9VIBR
193
0
22335
TrEMBL
-
A0A0H5QFQ3_NEIMI
563
0
63335
TrEMBL
-
W1DUZ1_KLEPN
68
0
7527
TrEMBL
-
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-20°C, 2 days, 20% loss of activity
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4°C, 0.1 M Tris-HCl, pH 7.3, 2 days, 20% loss of activity
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Ogata, M.; Arihara, K.; Yagi, T.
D-Lactate dehydrogenase of Desulfovibrio vulgaris
J. Biochem.
89
1423-1431
1981
Desulfovibrio vulgaris
brenda
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