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IUBMB CommentsThe enzyme from the sulfate-reducing bacterium Desulfovibrio vulgaris can also act on (R)-2-hydroxybutanoate.
The expected taxonomic range for this enzyme is: Nitratidesulfovibrio vulgaris
Synonyms
d-lactate:ferricytochrome c-553 oxidoreductase, more
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D-lactate:ferricytochrome C-553 oxidoreductase
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(R)-lactate + 2 ferricytochrome c-553 = pyruvate + 2 ferrocytochrome c-553 + 2 H+
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MetaCyc
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales)
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(R)-lactate:ferricytochrome-c-553 2-oxidoreductase
The enzyme from the sulfate-reducing bacterium Desulfovibrio vulgaris can also act on (R)-2-hydroxybutanoate.
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D-lactate + 1-methoxyphenazinium methyl sulfate
pyruvate + ?
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D-lactate + 2,6-dichlorophenolindophenol
pyruvate + ?
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D-lactate + 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride
pyruvate + ?
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D-lactate + ferricyanide
pyruvate + ferrocyanide
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D-lactate + ferricytochrome c-553
pyruvate + ferrocytochrome c-553
D-lactate + methylene blue
pyruvate + ?
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D-lactate + nitrotetrazolium blue
pyruvate + ?
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DL-2-hydroxybutyrate + ferricytochrome c-553
2-ketobutyrate + ferrocytochrome c-553
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additional information
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Substrates: no activity with: NAD+, NADP+, FAD, FMN, cytochrome C3 and O2. Enzyme does not have any diaphorase activity
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D-lactate + ferricytochrome c-553
pyruvate + ferrocytochrome c-553
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D-lactate + ferricytochrome c-553
pyruvate + ferrocytochrome c-553
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Substrates: starting enzyme for carbon and energy metabolism
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D-lactate + ferricytochrome c-553
pyruvate + ferrocytochrome c-553
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Substrates: starting enzyme for carbon and energy metabolism
Products: -
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Ferricytochrome c-553
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EDTA
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slight stimulation
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2.4
DL-2-hydroxybutyrate
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8
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D-lactate + nitrotetrazolium blue
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7 - 9
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pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity, D-lactate + nitrotetrazolium blue
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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-20°C, 2 days, 20% loss of activity
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4°C, 0.1 M Tris-HCl, pH 7.3, 2 days, 20% loss of activity
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Ogata, M.; Arihara, K.; Yagi, T.
D-Lactate dehydrogenase of Desulfovibrio vulgaris
J. Biochem.
89
1423-1431
1981
Nitratidesulfovibrio vulgaris
brenda
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